Physiological Characterization of Dicarboxylate-Induced Pleomorphic Forms of Bradyrhizobium japonicum

Abstract

When Bradyrhizobiumjaponicum 1-110 was transferred into medium containing 40 mM succinate or 40 mM fumarate, over 90% of the bacteria acquired a swollen, pleomorphic form similar to that of bacteroids. The induction of pleomorphism was dependent on the carbon substrate and concentration but was independent of the hydrogen ion and sodium ion concentration. Cell extracts of rod-shaped and pleomorphic cells contained enzymes required for sugar catabolism and gluconeogenesis. Variations in these enzyme profiles were correlated with the carbon source used and not with the conversion to the bacteroid-like morphology. Rod-shaped cells cultured on glucose or 10 mM succinate transported glucose and succinate; however, the pleomorphic cells behaved similarly to symbiotic bacteroids in that they lacked the ability to transport glucose and transported succinate at lower rates than did rod-shaped cells.

Interacting bactofilins impact cell shape of the MreB-less multicellular Rhodomicrobium vannielii

“…In addition, several bacteria adopt different morphotypes dependent on environmental cues or developmental stages, i.e. shapes are switched while cells undergo differentiation during their complex life cycles, which is finally surpassed by multicellular species whose assemblies consist of different cell types [2][3][4][5][6][7][8][9][10].…”