Physiological characterization and proline route genes quantification under long-term cold stress in Carrizo citrange

Primo-Capella, Amparo; Martínez-Cuenca, Mary-Rus; Gil-Muñoz, Francisco; Forner-Giner, María Ángeles

doi:10.1016/j.scienta.2020.109744

Cited by 18 publications

(14 citation statements)

References 65 publications

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“…For example, it is known that Poncirus trifoliata is one of more tolerant rootstocks to tristeza virus (CTV) which, when grafted with a CTV-sensitive variety, is capable of transmitting tolerance capacity [6,7]. In other words, rootstocks must be able to transmit a quality desired by farmers to a variety and, depending on the selected rootstock/variety combination, these skills will differ [8][9][10][11].…”

Section: Citriculture and Cold Stressmentioning

confidence: 99%

“…and Poncirus trifoliata (L. Raf), which possesses a certain degree of the cold tolerance from the Poncirus trifoliata parental [43]. Other works with the commercial rootstock Carrizo grafted onto Valencia delta seedless were carried out by María Ángeles Forner and Javier Terol's groups to demonstrate the rootstock influence under cold stress [9,44]. The metabolomic and transcriptomic study performed by these groups indicates that the mechanisms activated in plants shortly after cold exposure remain active in the long term.…”

Section: Citriculture and Cold Stressmentioning

confidence: 99%

“…In citrus, there are many examples shown in different citrus species that photosynthesis and photosystems are damaged in the presence of low temperatures: in Citrus junos [91], in Carrizo citrange [9,42], in Fortune mandarin and Ellendale tangor [118], in Citrus deliciosa and Poncirus trifoliata var. 'Pomeroy' [119], Citrus clementina [42], Citrus medica (fingered Citron) and sweet orange [120] and satsuma mandarin [121].…”

Section: Photosynthesis and Photoinhibition In Citrus Under Cold Stressmentioning

confidence: 99%

“…Apart from the proline amino acid conferring tolerance to cold, it has been found to be of much help for selecting tolerant genotypes in citrus [9,92,93,158]. In two works carried out by Abouzar Abouzari, two markers associated with proline content on chromosomes 5 and 8 (CT21 and GT03, respectively) were selected based on the association of the physiological results with molecular markers [92,93].…”

Section: Osmoprotectors In Citrus Under Cold Stressmentioning

confidence: 99%

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Cold Stress in Citrus: A Molecular, Physiological and Biochemical Perspective

2021

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Due to climate change, we are forced to face new abiotic stress challenges like cold and heat waves that currently result from global warming. Losses due to frost and low temperatures force us to better understand the physiological, hormonal, and molecular mechanisms of response to such stress to face losses, especially in tropical and subtropical crops like citrus fruit, which are well adapted to certain weather conditions. Many of the responses to cold stress that are found are also conserved in citrus. Hence, this review also intends to show the latest work on citrus. In addition to basic research, there is a great need to employ and cultivate new citrus rootstocks to better adapt to environmental conditions.

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Section: Citriculture and Cold Stressmentioning

confidence: 99%

Section: Citriculture and Cold Stressmentioning

confidence: 99%

Section: Photosynthesis and Photoinhibition In Citrus Under Cold Stressmentioning

confidence: 99%

Section: Osmoprotectors In Citrus Under Cold Stressmentioning

confidence: 99%

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Cold Stress in Citrus: A Molecular, Physiological and Biochemical Perspective

2021

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“…For this reason, it is very important to obtain new plant material that is able to face unfavorable situations, such as cold stress. In citrus, the grafting technique is widespread and used to face abiotic stresses [2][3][4][5]. Thus, the selection of a good rootstock is important and depends on the cultivation area [6].…”

Section: Introductionmentioning

confidence: 99%

Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks

2021

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Climate change and global warming are leading to a change in weather patterns toward hot and cold waves. Citrus fruits are a tropical or subtropical crop whose growth is altered by changes in weather patterns. Thus, in the present work, two experiments are evaluated to obtain a screening method to select citrus rootstocks that help us to select new low-temperature-tolerant plant materials. One cold experiment was carried out with the Poncirus trifoliata and Citrus macrophylla rootstocks at 4 °C for 4, 8, 24 and 56 h. A second experiment was performed at 4 °C for 5 days with subsequent acclimatization lasting 0, 5, 10 and 24 h. The expression of the cold response genes CAMTA1, CAMTA3, CAMTA5, CBF1, ICE1 and COR413 IM1 was quantified. The results showed that the best rootstock selection strategy was the second experiment, as a higher expression of the genes CAMTA3, CAMTA5, CBF1 and COR413 IM1 was seen in the tolerant genotype P. trifoliata. We quantified the gene expression of proline biosynthesis P5CS1, dOAT and the proline transporters PROT1 and PROT2; the concentration of the amino acid proline in leaves was also quantified. These results once again showed that the best experiment to differentiate between tolerant and sensitive rootstocks was the second experiment with acclimation time.

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