Physiological Characteristics of Medicago sativa L. in Response to Acid Deposition and Freeze-Thaw Stress

Bao, Guozhang; Ao, Qi; Bao, Yishu; Zheng, Yue; Feng, Xiaoxia; Ding, Xuemei

doi:10.1007/s11270-017-3561-8

Cited by 11 publications

(7 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was reported that cold treatment could increase accumulation of soluble protein (Castonguay et al, 1995). Bao et al (2017) had pointed out that an increased protein content of alfalfa ( M. sativa cv. Dongmu) prevents damage from cold stress, and soluble sugar accumulation was associated with enhanced freezing tolerance in curly kale ( Brassica oleracea L. var acephala ; Steindal et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Effect of Rhizobium Symbiosis on Low-Temperature Tolerance and Antioxidant Response in Alfalfa (Medicago sativa L.)

Liu

Geng²,

Sha

et al. 2019

Front. Plant Sci.

View full text Add to dashboard Cite

Low temperature-induced stress is a major environmental factor limiting the growth and development of plants. Alfalfa ( Medicago sativa L.) is a legume well known for its tolerance of extreme environments. In this study, we sought to experimentally investigate the role of rhizobium symbiosis in alfalfa’s performance under a low-temperature stress condition. To do this, alfalfa “Ladak + ” plants carrying active nodules (AN), inactive nodules (IN), or no nodules (NN) were exposed to an imposed low temperature stress and their survivorship calculated. The antioxidant defense responses, the accumulation of osmotic regulation substances, the cell membrane damage, and the expression of low temperature stress-related genes were determined in both the roots and the shoots of alfalfa plants. We found that more plants with AN survived than those with IN or NN under the same low temperature-stress condition. Greater activity of oxidation protective enzymes was observed in the AN and IN groups, conferring higher tolerance to low temperature in these plants. In addition, rhizobia nodulation also enhanced alfalfa’s ability to tolerate low temperature by altering the expression of regulatory and metabolism-associated genes, which resulted in the accumulation of soluble proteins and sugars in the nodulated plants. Taken together, the findings of this study indicate that rhizobium inoculation offers a practical way to promote the persistence and growth potential of alfalfa “Ladak + ” in cold areas.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Rhizobium Symbiosis on Low-Temperature Tolerance and Antioxidant Response in Alfalfa (Medicago sativa L.)

Liu

Geng²,

Sha

et al. 2019

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…The activities of CAT, SOD and POD were determined with the CAT, SOD and POD kits provided by Nanjing Jiancheng Biological Institute [37]. A parallel sample (around 0.25 g) were randomly taken and ground to a homogenate with 5 ml phosphate buffer on ice.…”

Section: Malondialdehyde (Mda) Contentmentioning

confidence: 99%

Responses of H. vulgare L. Seedlings to Basic Salt and Drought under Freeze-thaw Condition

Tang¹,

Bao²,

Yan³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background Qinghai-Tibet Plateau is known for characteristics of high altitude, low rainfall and varying temperature, and the crops in this area are susceptible to abiotic stresses such as drought, basic salt and freeze-thaw that caused damages in different perspectives such as the permeability of biological membrane, osmotic adjustment, and antioxidant enzyme system. Hordeum vulgare L. is an indispensable crop in plateau and plays an important role in agricultural ecosystem as well.Result In this experiment, H. vulgare L. was used as experimental material and the physiological characteristics soluble protein (SP) content, malondialdehyde (MDA) content, antioxidant enzyme activity and relative water content (RWC) of seedlings were examined under freeze-thaw condition combined with drought and alkali stress.Conclusion Research results indicated that under the combined stresses of basic salt and drought, H. vulgare L. seedlings were damaged by lipid peroxidation, weakened superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities, while enhanced osmotic adjustment ability in plants cell. It was suggested that, in agricultural management, the simultaneous occurrence of two stresses, basic salt and drought, should be avoided in the early stage of H. vulgare L. planting to reduce the physiological stress on plants.

show abstract

“…Sulfuric acid anthrone was used to measure the content of the soluble sugar as previously described (Bao et al 2017). 0.1 g of fresh sample was taken and put into a large test tube.…”

Section: Rwc(%)=(tw-fw)/(tw-dw)×100%mentioning

confidence: 99%

Physiological Effects of Different Stubble Height and Freeze-Thaw Stress on Secale cereale L. Seedlings

Zhang

Bao

Zhang

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background: As a biennial plant, Secale cereale L is usually harvested in the autumn in the northern part of China where the temperature difference between day and night is of great disparity Through the pot experiment, the seedlings were cut to 2, 6 and 10 cm stubble height, and the simulated freeze-thaw (FT) stress (10/-5℃) was carried out after 6 days regrowth. The physiological effects of FT with different stubble height were revealed by analyzing the relative water content (RWC), osmotic adjustment substance concentration (soluble sugar and protein), membrane peroxidation (MDA) and catalase (CAT) activity. Results: The results demonstrated that under freeze stress (-5℃), the content of soluble protein and MDA decreased and the seedlings of 2 cm treatment kept higher level of soluble protein and MDA, while the seedlings of 6 and 10 cm treatments kept higher level of the RWC, soluble sugar content, and CAT activity. After FT stress, the content of soluble sugar and protein, RWC in the 6 cm treatment were higher than those in 2 cm and 10 cm treatments, and the CAT activity in 10 cm treatment was the highest while the MDA content is lower.Conclusion: These data suggest that keeping high stubble height is more adaptive for short-term FT stress.

show abstract

Physiological Characteristics of Medicago sativa L. in Response to Acid Deposition and Freeze-Thaw Stress

Cited by 11 publications

References 6 publications

Effect of Rhizobium Symbiosis on Low-Temperature Tolerance and Antioxidant Response in Alfalfa (Medicago sativa L.)

Effect of Rhizobium Symbiosis on Low-Temperature Tolerance and Antioxidant Response in Alfalfa (Medicago sativa L.)

Responses of H. vulgare L. Seedlings to Basic Salt and Drought under Freeze-thaw Condition

Physiological Effects of Different Stubble Height and Freeze-Thaw Stress on Secale cereale L. Seedlings

Contact Info

Product

Resources

About