Physiological characteristics of four methylotrophic bacteria and their potential use in single-cell protein production

Banat, Ibrahim M.; Al-Awadhi, N.; Hamdan, I. Y.

doi:10.1007/bf01741838

Cited by 8 publications

(5 citation statements)

References 6 publications

(3 reference statements)

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“…The authors suggested this alternative form of SCP production for animal feed, as well as this strategy for the management of animal waste. In the present study, almost all amino acids were identified, and histidine was found at the highest concentration ( Table 3 ( 43 - 46 )).…”

Section: Resultssupporting

confidence: 55%

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Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest

Simões

Fernandes

Barreto

et al. 2022

Food Technol. Biotechnol. (Online)

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Research background. This study aimed to monitor the growth of the methylotrophic bacteria Methylobacterium organophilum in a culture medium with methanol as a carbon source and to verify the production of unicellular proteins and other biomolecules, such as carotenoids, exopolysaccharides and polyhydroxyalkanoates, making a compound more attractive as food intended for animal feed. Experimental approach. Microbial growth was studied in shaken flasks to evaluate bacterial growth with different carbon: nitrogen ratios (C: N) and determine the best results in terms of cell volumetric productivity and substrate consumption rate. This condition was to be further applied in an in a fed-batch operating bioreactor system, which depicted the kinetic profile of cell growth, which concentrations were determined by dry cell weight, methanol consumption, measured by HPLC analysis, accumulation of carotenoids, which was analyzed by mass spectrometry, and the production of exopolysaccharide, which was characterized in terms of its chemical composition and submitted to chemical rheological analysis. Results and conclusions. The best experimental condition was verified using an initial methanol concentration of 7 g/L in the culture medium. This same initial substrate concentration was used in the bioreactor in the fed-batch operation, and the biomass concentration was 5 g/L, after 60 hours of cultivation. The accumulation of carotenoids associated with cell growth was monitored, reaching a concentration of 1.6 mg/L at the end of the process. These pigments were then analyzed and characterized as a set of xanthophylls (oxidized carotenoids). In addition, two other products were identified during the fed-batch operation: an exopolysaccharide (EPS), which reached a concentration of 8.9 g/L at the end of the culture, and an intracellular granular structure which was detected by transmission electron microscopy (TEM), suggesting the accumulation of polyhydroxyalkanoate (PHA), most likely polyhydroxybutyrate. Novelty and scientific contribution. M. organophilum demonstrated a unique ability to produce compounds of commercial interest, leading to a genuine understanding that this species has a metabolism distint. The use of Methylobacterium organophilum makes room for platform integration in the context of biorefineries as a result of its distinct metabolic diversity.

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Section: Resultssupporting

confidence: 55%

“… a= Methylobacterium organophilum (this work), b=five strains of the genus Methylophilus ( 46 ), c= Rhodocyclus gelatinosus ( 43 ), d= Saccharomyces cerevisiae ( 44 ), e= Rhodopseudomonas faecalis ( 45 ) …”

Section: Resultsmentioning

confidence: 99%

Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest

Simões

Fernandes

Barreto

et al. 2022

Food Technol. Biotechnol. (Online)

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“…In many respects, this is not surprising since these isolates have been selected on the basis of their high yields and fast growth rate for use in single-cell protein production [2]. This proves that methanol is assimilated in the RMP pathway only.…”

Section: Discussionmentioning

confidence: 93%

Methanol metabolism and ammonia assimilation in four methylophilns strains

Elrayes

Banat

Hamdan

1991

Acta Biotechnologica

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Methanol assimilation and disaimilation pathways and ammonia assimilntion pathway were investigated in four obligate methanol-utilizing bacteria through the detection of key enzymes. Both hexulose phoephate syntheteae and hexulose phosphate isomerase, key enzymes of the ribulose monophosphate pathway (RE') for methnnol assimilation were detected whereas four key enzymes (hydroxy pyruvate reductase, Locitrate lyase, malyl-CoA-lyase and glyoxylate aminotransferase) that are characteristic of the serine assimilation pathway were absent. Key enzymes for the two methanol dissimilation pathwap, the linear sequence enzymes formaldehyde and formate dehydrogenase and the RMP cyclic sequence enzymes glucose-6-phosphate dehydrogenase and 6-phosphate gluconate dehydrogenase were all detected. Ammonia was assimilated via the glutamate dehydrogenase pathway and not vin the glutamine synthetase and glutamate synthase pathway.

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“…Cultivation conditions such as temperature, pH, oxygen and dilution rate also have a profound influence on the growth rate, macromolecular composition and the levels of their intracellular metabolites (Tempest and Hunter 1965;Bull and Brown 1979;Banat et al 1989), and will finally control the biomass yield coefficient. Our mixed bacterial culture had optimum growth and high production yields with negligible polysaccharide formation at 35°-39°C and at pH 6.8; it also grew well between pH 6.5 and 7.2.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have shown that maximum growth rate, biomass yield and accumulation of storage compounds in cells are affected by the elemental composition of the medium, such as N, P, S, Ca, Mg, Fe, Cu and Zn (Duguid and Wilkinson 1953;Wilkinson et al 1954;Corpe 1964;Mateles and Battat 1974;Goldberg and Er-el 1981;Tempest and Wouters 1981 ;Banat et al 1989). In addition, varOffprint requests to: A. S. Abu-Ruwaida ious cultivation conditions such as temperature, pH, dissolved oxygen and dilution rate affect specific growth rate, biomass yield and accumulation of storage compounds (Alroy and Tannenbaum 1973;H~iggstr6m 1977;Bull and Brown 1979;Litchfield 1979;Lallai et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Chemostat optimization of biomass production of a mixed bacterial culture utilizing methanol

Aburuwaida

Banat

Hamdan

1990

Appl Microbiol Biotechnol

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A continuous culture technique was used to optimize the medium composition and growth conditions of a mixed bacterial culture utilizing methanol. The improved medium resuited in satisfactory growth, high-yield coefficients and gave a product containing reduced polysaccharide concentrations. Optimal growth and biomass yields occurred at pH 6.8 a temperature of 37°C and dissolved oxygen at >20% saturation. The maximum growth rate was 0.58 h -1 and maximum biomass yield 0.48 g g-1. The protein content of the product ranged between 81%-83%, and nucleic acid content between 10%-12%, increasing with growth rate. The amino acid profile of the mixed culture product m e t and, in some cases, exceeded the UN Food and Agricultural Organization standard, indicating a good source of feed protein.

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Physiological characteristics of four methylotrophic bacteria and their potential use in single-cell protein production

Cited by 8 publications

References 6 publications

Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest

Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest

Methanol metabolism and ammonia assimilation in four methylophilns strains

Chemostat optimization of biomass production of a mixed bacterial culture utilizing methanol

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