Physiological Characterisation of Alternaria brassicicola causing Alternaria Leaf Spot in Cabbage

Kiran, V.N.S. Gunda Madhu; Thara, Susha S.; Pavan, Sree

doi:10.20546/ijcmas.2018.710.317

Cited by 3 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On MEA, the initial color of the colony was light greenish to brown and off white at the colony edges in a 24-h dark environment. The continuous light reduced the sporulation of A. alternata , and the colony appeared light brownish with a grayish to off white color supported by a previous study ( Kiran et al, 2018 ).…”

Section: Discussionsupporting

confidence: 83%

Characterization and toxicological potential of Alternaria alternata associated with post-harvest fruit rot of Prunus avium in China

Ahmad,

Xing,

Cao

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Post-harvest fruit rot caused by Alternaria species is one of the most important threats to the fruit industry. Post-harvest rot on sweet cherry (Prunus avium) fruit was observed in the fruit markets of the Haidian district of Beijing, China. The fungal isolates obtained from the infected sweet cherry fruits matched the descriptions of Alternaria alternata based on the morphology and multi-gene (ITS, endo-PG, and Alta1) sequence analysis. Pathogenicity tests indicated that ACT-3 was the most virulent isolate, exhibiting typical post-harvest fruit rot symptoms. Physiological studies revealed that the optimal conditions for the growth of ACT-3 were temperature of 28°C, water activity of 0.999, and pH of 8 with 87, 85, and 86 mm radial growth of ACT-3 on a potato dextrose agar (PDA) medium, respectively, at 12 days post-inoculation (dpi). Moreover, the fungus showed the highest growth on a Martin agar medium (MAM) modified (85 mm) and a PDA medium (84 mm) at 12 dpi. The proliferation of the fungus was visualized inside the fruit tissues by confocal and scanning electron microscope (SEM), revealing the invasion and destruction of fruit tissues. Alternaria mycotoxins, tenuazonic acid (TeA), and alternariol (AOH) were detected in five representative isolates by HPLC analysis. The highest concentrations of TeA (313 μg/mL) and AOH (8.9 μg/mL) were observed in ACT-6 and ACT-3 isolates, respectively. This study is the first to present a detailed report on the characteristics and proliferation of A. alternata associated with sweet cherry fruit rot and the detection of toxic metabolites.

show abstract

Section: Discussionsupporting

confidence: 83%

Characterization and toxicological potential of Alternaria alternata associated with post-harvest fruit rot of Prunus avium in China

Ahmad,

Xing,

Cao

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Both strains were incubated for two weeks. The temperature during the day (14 h) and night (10 h) was 21 and 18 °C (± 1 °C), respectively (Kiran et al 2018). Before infection, the spores were prepared in sterile water and counted in a hemocytometer.…”

Section: Fungal Growthmentioning

confidence: 99%

Necrotrophic fungal infection affects indolic glucosinolate metabolism in Brassica rapa

et al. 2023

View full text Add to dashboard Cite

Brassica species contains sulfur-containing secondary compounds including glucosinolates which might protect plants from pathogens. In the present investigation, the first leaves of Brassica rapa were grown in different situations such as sulfatesufficient and deprived conditions, and infected with two types of fungi namely, Alternaria brassicicola and Botrytis cinerea as the specialist Brassica pathogen and generalist pathogen, respectively. The glucosinolates level was locally increased mainly due to indolic glucosinolates when the plant was infected with both fungi. This increase was in line with the increase in the expression of the genes including CYP79B2, CYP79B3, and CYP83B1 which are responsible for the biosynthesis of indolic glucosinolates and their regulation (MYB34 and MYB51). However, the locally induced indolic glucosinolates in plants infected with A. brassicicola were substantially higher than those of the plants infected with B. cinerea. The expression of the genes responsible for the biosynthesis of indolic glucosinolates was increased by infection of plant with A. brassicicola. The increase in the content of indolic glucosinolate occurred in the second leaf and roots, demonstrating a systemic response to fungal infection. Upon infection of plants with fungi, the content of both glucosinolates was reduced, while the expression of the most genes responsible for the biosynthesis of indolic glucosinolates was enhanced in plants infected with A. brassicicola. This may indicate that indolic glucosinolates are important in response to necrotrophic fungi in Brassica.

show abstract

“…In contrast, the pathogen is considered occasional or weak and can establish itself late on plants of Resedaceae and Solanaceae, on drying latex of injured trunks of Hevea sp., or in the soil (Lawrence et al, 2015). The development of A. brassicicola is influenced by several environmental factors: the optimal temperature for its growth is 25°C, whereas a temperature of 35°C completely inhibits its development (Kiran et al, 2018). Also, the growth and sporulation of the parasite are strongly affected by high light intensities (˃ 20 lux) (Kiran et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The development of A. brassicicola is influenced by several environmental factors: the optimal temperature for its growth is 25°C, whereas a temperature of 35°C completely inhibits its development (Kiran et al, 2018). Also, the growth and sporulation of the parasite are strongly affected by high light intensities (˃ 20 lux) (Kiran et al, 2018). Its conidial chains of are moderately long, simple or branched.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Alternaria brassicicola isolated from tomato in Burkina Faso, and use of two essential oils for its control in vitro

Sirima¹,

Sereme²,

Koïta³

et al. 2021

Afr. J. Agric. Res.

View full text Add to dashboard Cite

Physiological Characterisation of Alternaria brassicicola causing Alternaria Leaf Spot in Cabbage

Cited by 3 publications

References 6 publications

Characterization and toxicological potential of Alternaria alternata associated with post-harvest fruit rot of Prunus avium in China

Characterization and toxicological potential of Alternaria alternata associated with post-harvest fruit rot of Prunus avium in China

Necrotrophic fungal infection affects indolic glucosinolate metabolism in Brassica rapa

Characterization of Alternaria brassicicola isolated from tomato in Burkina Faso, and use of two essential oils for its control in vitro

Contact Info

Product

Resources

About