Physiological changes of Fas expression in peripheral lymphocyte subsets during the menstrual cycle

Shinoda, Rie; Watanabe, Mikio; Nakamura, Yukiyo; Maruoka, Hayato; Kimura, Yoshiki; Iwatani, Yoshinori

doi:10.1016/s0165-0378(03)00107-4

Cited by 6 publications

(6 citation statements)

References 33 publications

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“…Peripheral blood samples were collected from the nonpregnant women during the follicular phase of the menstrual cycle, as hormone status during the luteal phase is comparable to that during pregnancy. 37 However, a previous study 38 reported that Treg subsets expand during the follicular phase, reaching a peak immediately before ovulation (to induce immune tolerance to facilitate implantation) and then decline in the subsequent luteal phase. This may have contributed to the absence of significant differences in Treg numbers between the nonpregnant and pregnant groups.…”

Section: Discussionmentioning

confidence: 98%

Regulatory T Cells Show Dynamic Behavior During Late Pregnancy, Delivery, and the Postpartum Period

et al. 2017

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Regulatory T cells (Tregs) are critical immunomodulators during early pregnancy by preventing maternal T-cell activation against fetal cells. However, how populations of maternal Tregs vary during and after pregnancy in humans is still unclear. Therefore, we investigated Treg subsets in the peripheral blood of pregnant women from late pregnancy through the postpartum period. To accomplish this, the following circulating Treg subsets were analyzed in 43 healthy pregnant women and 35 nonpregnant women by flow cytometry during the third trimester, on the day of delivery, and postpartum: CD4CD25, CD4CD25Foxp3, and CD4CD25CD127. Additionally, the expression levels of the transcription factor Foxp3 in CD4CD25 Treg were analyzed. We have found that CD4CD25 Treg subset significantly decreased in the pregnant women on the day of delivery relative to the third trimester ( P < .05), and that all Treg subsets significantly increased postpartum compared to the third trimester and the day of delivery ( P < .05). Moreover, the Foxp3 expression ratios within the CD4CD25 Treg subset decreased during pregnancy and until delivery compared to those measured in the nonpregnant women and significantly increased postpartum compared to the third trimester and the day of delivery ( P < .05). Thus, despite their established role in offering immunoprotection to the fetus in early pregnancy, the number of circulating Tregs also varies from late pregnancy to the postpartum period. Our results offer an explanation for the possible effects of pregnancy on the clinical outcomes of some autoimmune diseases during the postpartum period.

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Section: Discussionmentioning

confidence: 98%

Regulatory T Cells Show Dynamic Behavior During Late Pregnancy, Delivery, and the Postpartum Period

et al. 2017

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“…To characterize B cell subsets from late pregnancy to post-partum, peripheral blood samples were collected from all of the pregnant women at each planned visit: the “3rd trimester” sample was collected at visit 1, the “on delivery day” sample was collected at visit 2 (immediately after delivery, within 15 min after placental expulsion and oxytocin administration), and the “post-partum” sample was collected at visit 3. A peripheral blood sample was collected from the non-pregnant women at the planned visit, which took place during the follicular phase of their menstrual cycle because hormone status during the luteal phase is similar to that during pregnancy [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Characterization of B cells in healthy pregnant women from late pregnancy to post-partum: a prospective observational study

Lima

Martins

Leandro

et al. 2016

BMC Pregnancy Childbirth

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BackgroundB cells play a role in pregnancy due to their humoral and regulatory activities. To our knowledge, different maturational stages (from transitional to memory) of circulating B cell subsets have not yet been characterized (cell quantification and phenotype identification) in healthy pregnant women. Thus, the objective of our study was to characterize these subsets (as well as regulatory B cells) from late pregnancy to post-partum and to compare them with the circulating B cells of non-pregnant women.MethodsIn all of the enrolled women, flow cytometry was used to characterize the circulating B cell subsets according to the expression of IgD and CD38 (Bm1-Bm5 classification system). Regulatory B cells were characterized based on the expression of surface antigens (CD24, CD27, and CD38) and the production of IL-10 after lipopolysaccharide stimulation.ResultsCompared to the absolute counts of B cells in the non-pregnant women (n = 35), those in the pregnant women (n = 43) were significantly lower (p < 0.05) during the 3rd trimester of pregnancy and on delivery day (immediately after delivery). The percentages of these cells on delivery day and at post-partum were significantly lower than those in the non-pregnant women.In general, the absolute counts and percentages of the majority of the B cell subsets were significantly lower in the 3rd trimester of pregnancy and on delivery day than in the non-pregnant women. However, these counts and percentages did not differ significantly between the post-partum and the non-pregnant women.The most notable exceptions to the above were the percentages of naïve B cells (which were significantly higher in the 3rd trimester and on delivery day than in the non-pregnant women) and of CD24hiCD38hi regulatory B cells (which were significantly higher in the post-partum than in the non-pregnant women).ConclusionAccording to our study, the peripheral B cell compartment undergoes quantitative changes during normal late pregnancy and post-partum. Such findings may allow us to better understand immunomodulation during human pregnancy and provide evidence that could aid in the development of new strategies to diagnose and treat pregnancy-associated disturbances. Our findings could also be useful for studies of the mechanisms of maternal responses to vaccination and infection.Electronic supplementary materialThe online version of this article (doi:10.1186/s12884-016-0927-7) contains supplementary material, which is available to authorized users.

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“…The increased expressions of APO-1/Fas antigen on T lymphocytes and NK cells in the luteal phase of the menstrual cycle might reflect aspects of the phenomenon of preparation for blastocyst implantation [29]. The lower rate of T cell apoptosis could correlate with the downregulation of APO-1/Fas antigen.…”

Section: Discussionmentioning

confidence: 99%

T CD3⁺CD8⁺Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy

Darmochwał-Kolarz

Sobczak

Pożarowski

et al. 2014

Journal of Immunology Research

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Aims. Normal human pregnancy is a complex process of many immunoregulatory mechanisms which protect fetus from the activation of the maternal immune system. The aim of the study was to investigate the apoptosis of lymphocytes in peripheral blood of normal pregnant patients and healthy nonpregnant women. Methods. Sixty pregnant women and 17 nonpregnant women were included in the study. Lymphocytes were isolated and labeled with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies. Apoptosis was detected by CMXRos staining and analyzed using the flow cytometric method. Results. We found significantly higher apoptosis of total lymphocytes in peripheral blood of pregnant patients when compared to healthy nonpregnant women. The percentage of apoptotic T CD3+CD8+ cells in the first trimester was significantly higher when compared to the third trimester of normal pregnancy. The ratio of T CD3+CD4+ : T CD3+CD8+ apoptotic lymphocytes was significantly lower in the first trimester when compared to other trimesters of pregnancy and to both of the phases of the menstrual cycle. Conclusions. The higher apoptosis of T CD3+CD8+ lymphocytes and the lower ratio of T CD3+CD4+ : T CD3+CD8+ apoptotic cells in the first trimester of normal pregnancy may suggest a higher susceptibility of T CD3+CD8+ cells for apoptosis as a protective mechanism at the early stage of pregnancy.

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Physiological changes of Fas expression in peripheral lymphocyte subsets during the menstrual cycle

Cited by 6 publications

References 33 publications

Regulatory T Cells Show Dynamic Behavior During Late Pregnancy, Delivery, and the Postpartum Period

Regulatory T Cells Show Dynamic Behavior During Late Pregnancy, Delivery, and the Postpartum Period

Characterization of B cells in healthy pregnant women from late pregnancy to post-partum: a prospective observational study

T CD3⁺CD8⁺Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy

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Physiological changes of Fas expression in peripheral lymphocyte subsets during the menstrual cycle

Cited by 6 publications

References 33 publications

Regulatory T Cells Show Dynamic Behavior During Late Pregnancy, Delivery, and the Postpartum Period

Regulatory T Cells Show Dynamic Behavior During Late Pregnancy, Delivery, and the Postpartum Period

Characterization of B cells in healthy pregnant women from late pregnancy to post-partum: a prospective observational study

T CD3+CD8+Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy

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T CD3⁺CD8⁺Lymphocytes Are More Susceptible for Apoptosis in the First Trimester of Normal Human Pregnancy