Physiological changes in the adenosine deaminase activity, antioxidant and inflammatory parameters in pregnant cows and at post‐partum

Fávero, Juscivete F.; Silva, Aleksandro S. Da; Bottari, Nathieli B.; Schetinger, Maria Rosa Chitolina; Morsch, Vera Maria; Baldissera, Matheus D.; Stefani, Lenita M.; Machado, Gustavo

doi:10.1111/jpn.12917

Cited by 11 publications

(9 citation statements)

References 46 publications

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“…In our study, calving significantly increased ADA1 isoenzyme in both serum and saliva. An increase of ADA in serum after calving had been previously described [43], and therefore ADA has been proposed to be a marker of a proinflammatory status of the animal due to calving [44]. To the authors' knowledge, this is the first report in which salivary ADA has been also reported to increase after calving in cows, being this change of higher magnitude of that reported in serum.…”

Section: Discussionmentioning

confidence: 51%

Adenosine deaminase (ADA) characterization in saliva and serum samples of different animal species

Contreras-Aguilar¹,

Tvarijonaviciute²,

Monkevičienė³

et al. 2020

Preprint

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Background The enzymatic activity of total adenosine deaminase (tADA) and its isoenzymes ADA1 and ADA2 were studied in canine, equine, porcine and bovine serum and saliva. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) was used as a specific ADA1 inhibitor at different concentrations, and the distribution of the isoenzymes in serum and saliva was studied in different inflammatory situations in each species.Results Total ADA was present in serum and saliva of healthy animals of the four species. EHNA concentration of 0.47mM was needed for ADA1 inhibition in canine and porcine samples (serum and saliva) and bovine saliva, whereas for equine saliva 0.94mM was needed. ADA2 activity was not detected in bovine serum and was very low or absent in equine serum and bovine saliva. An automated procedure to measure ADA2 consisting of adding EHNA to a commercial reagent for tADA measurement provided repetitive (coefficients of variation <8.8% in serum and <10% in saliva) and accurate (linearity under dilution approaches gave R 2 >0.90) results, being equivalent to a manual incubation of the sample with EHNA at a similar concentration. Salivary tADA, as well as ADA1 and ADA2, were higher in dogs with leishmaniosis, horses with acute abdominal disease and pigs with lameness than in healthy animals. ADA showed significant correlation with serum ferritin in dogs ( r = 0.602, P < 0.01; r = 0.555, P < 0.05; and r = 0.632, P < 0.01; respectively for tADA, ADA1 and ADA2) and serum C-reactive protein in pigs ( r = 0.700, P < 0.01, for both tADA and ADA1; r = 0.770, P < 0.001, for ADA2), whereas salivary ADA2 significantly correlated with serum amyloid A in the horse ( r = 0.649, P < 0.01). In the cow, salivary tADA and ADA1 significantly increased after calving, being correlated with total white blood cell count ( r = 0.487, P < 0.05, both).Conclusions ADA activity, as well as its isoenzymes, can be measured in serum and saliva of different animal species, and the salivary levels of these analytes could potentially be inflammatory biomarkers in these species. BackgroundAdenosine deaminase (ADA, EC number 3.5.4.4) is an enzyme that can be found in several tissues and fluids and it has two different isoenzymes. The isoenzyme ADA1 is mainly present in lymphoid

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Section: Discussionmentioning

confidence: 51%

Adenosine deaminase (ADA) characterization in saliva and serum samples of different animal species

Contreras-Aguilar¹,

Tvarijonaviciute²,

Monkevičienė³

et al. 2020

Preprint

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“…The occurrence in ammatory response during the parturition process has previously been described for sows [29,30], mares [31], cows [32] and humans [33]. Due to the signi cant changes observed in ADA1, ADA2 and tADA activities throughout the periparturient period in both the PDS + and the PDS ÷ sows, saliva ADA seems to re ect this in ammatory response in sows.…”

Section: Discussionmentioning

confidence: 67%

“…Another explanation could be in ammation-related synthesis of ADA, as suggested by our results. The relation to in ammation is, however, not entirely clear, as although Fávero et al [32] demonstrated increased ADA activities in cows at the end of pregnancy and after calving, other reports were not able to demonstrate any increase in serum ADA during in ammatory conditions [22,23].…”

Section: Discussionmentioning

confidence: 93%

Changes of Adenosine Deaminase Activity in Serum and Saliva around Parturition in Sows with and without Postpartum Dysgalactia Syndrome

Kaiser

Dahl

Jacobsen

et al. 2021

Preprint

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BackgroundPostpartum dysgalactia syndrome (PDS) is associated with a significantly higher activation of the inflammatory and stress response at parturition than in the healthy sow. Therefore, reliable and possibly non-invasive biomarkers for substantial increases of inflammation are searched to support the PDS diagnosis. This report studies the possible changes of the inflammatory marker enzyme adenosine deaminase (ADA) in serum and saliva of 38 PDS positive sows (PDS+) and 38 healthy sows (PDS÷). Sampling was performed every 24 h from 60 h before to 36 h after parturition. Isoenzyme 1 (ADA1) and isoenzyme 2 (ADA2), as well as total ADA (tADA) were measured and their statistical association with several serum and saliva biomarkers of inflammation and stress was investigated.ResultsCompared to a baseline (60 h to 36 prepartum), salivary activities of ADA1, ADA2 and tADA increased significantly over time in both PDS+ and PDS÷ sows, reaching their peaks after parturition. In serum from PDS÷ sows, no changes were observed over time in either ADA1, ADA2 or tADA. In PDS+ sows, serum ADA2 activity decreased temporarily after parturition followed by a significant increase compared to baseline. ADA1, ADA2 and tADA were all significantly associated with several inflammatory biomarkers and ADA1 in serum was associated with serum cortisol. Although serum activity was higher in PDS+ than in PDS÷ sows, the differences were not statistically significant. Further, no difference was noted between the groups in the analyses of saliva. Conclusions Salivary ADA1 and ADA2 increased in all sows after parturition, potentially as a response to the postpartum inflammation. However, no difference in the activity of ADA1, ADA2 and tADA were found between PDS+ and PDS÷ sows indicating inability to diagnose PDS under the conditions described in this report.

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“…Pei et al (2018) suggest that A2AR plays a role in tissue in lammation, which is attributable to A2AR proin lammatory activation. The signi icant high level of ADA serum level in adenosine group compared to control one can support this role of adenosine since that ADA released principally by in lammatory cell (Lee et al, 2020) and its activity has important role as in lammatory modulators (Fávero et al, 2018). Adenosine is closely connected to the A2AR activation (Al-Moula et al, 2012) an increased extracellular adenosine concentration promotes the up regulation of A2AR signaling that enhance the synthesis of in lammatory mediators like IL-1b (Meng et al, 2019), suggesting that adenosine enhances in lammatory response via A2AR-mediated signaling.…”

Section: Resultsmentioning

confidence: 78%

Effects of Adenosine A2A Receptor Agonist on Histopathology of Some Oral Tissues in Rabbits

Al-Mashhadane

2020

ijrps

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Adenosine is a protective regulator that act endogenously to restore equilibrium of cellular energy in response to tissue trauma. It can perform such function of different systems in the body by activation of adenosine receptors. Study the effects of systemic administration of the adenosine on tongue and salivary glands tissues in the rabbit model. Thirty male rabbits of body weight of 1.5 ± 0.25kg were included in the study. In control group (15 animals), one ml of distilled water was injected intraperitoneally while in treatment group (15 animals) were injected by adenosine intraperitoneally at a dose of one mg/ml, All animals were sacrificed after 30 days. Serum samples were separated and used for analysis of adenosine deaminase (ADA)and glutathione(GSH). Tissue samples sections from tongue and salivary glands were stained with hematoxylin-eosin (H&E) and examined under a light microscope for histological changes by a blinded pathologist. Histological sections in treatment group showed congestion of blood vessels and infiltration of inflammatory cells with mild hemorrhage among acini of salivary glands. Increased level of adenosine in the body microenvironment may affect tongue and salivary glands tissues by modulating some processes including inflammation and blood vessels.

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Physiological changes in the adenosine deaminase activity, antioxidant and inflammatory parameters in pregnant cows and at post‐partum

Cited by 11 publications

References 46 publications

Adenosine deaminase (ADA) characterization in saliva and serum samples of different animal species

Adenosine deaminase (ADA) characterization in saliva and serum samples of different animal species

Changes of Adenosine Deaminase Activity in Serum and Saliva around Parturition in Sows with and without Postpartum Dysgalactia Syndrome

Effects of Adenosine A2A Receptor Agonist on Histopathology of Some Oral Tissues in Rabbits

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