Physiological changes and transcriptome profiling in Saccharum spontaneum L. leaf under water stress and re-watering conditions

Abstract

As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source to various biotic and abiotic stresses. However, little has been reported on the mechanism of drought tolerance in S. spontaneum. Herein, the physiological changes of S. spontaneum GXS87-16 at three water-deficit levels (mild, moderate, and severe) and after re-watering during the elongation stage were investigated. RNA sequencing was utilized for global transcriptome profiling of GXS87-16 under severe drought and re-watered conditions. There were significant alterations in the physiological parameters of GXS87-16 in response to drought stress and then recovered differently after re-watering. A total of 1569 differentially expressed genes (DEGs) associated with water stress and re-watering were identified. Notably, the majority of the DEGs were induced by stress. GO functional annotations and KEGG pathway analysis assigned the DEGs to 47 GO categories and 93 pathway categories. The pathway categories were involved in various processes, such as RNA transport, mRNA surveillance, plant hormone signal transduction, and plant-pathogen interaction. The reliability of the RNA-seq results was confirmed by qRT-PCR. This study shed light on the regulatory processes of drought tolerance in S. spontaneum and identifies useful genes for genetic improvement of drought tolerance in sugarcane.

Comparative Effects of Individual and Consortia Plant Growth Promoting Bacteria on Physiological and Enzymatic Mechanisms to Confer Drought Tolerance in Maize (Zea mays L.)

“…Therefore, the utilization of plant growth promoting potential of microbes may offer a sustainable answer to (Rubin et al 2017;Joshi et al 2020). As the water deficit stress affects the processes of plant growth at physiological, biochemical, and molecular levels (Li et al 2021), the microbe mediated protective mechanisms augment stress tolerance by increasing the lateral root formation and regulating the photosynthetic capacity and enzymatic activity of antioxidants (Shabbir et al 2019). It is a well-known fact that the inoculation of crop seeds with PGPB strains enhances the water and nutrient availability to facilitate plant growth in dry soils (Ansari et al 2021).…”

Photosynthetic gas exchange, plant water relations and osmotic adjustment of three tropical perennials during drought stress and re-watering

“…I pes-caprae is an herbaceous creeper with succulent stems. Differences in plant responses to drought stress and re-watering following drought stress have been associated with species depending on their morphological, physiological and biochemical traits [43][44][45]. For instance, leaf size and even leaf shape, are potential adaptive mechanisms, which may help to limit water loss [43].…”

Transcriptomic and Proteomic Landscape of Sugarcane Response to Biotic and Abiotic Stressors

“…RNA-Seq identified a total of 1569 DEGs, with the majority being induced by the water deficit. GO and KEGG analyses assigned these DEGs to 47 GO categories and 93 metabolic pathways associated with phytohormone signal transduction, plant-pathogen interaction, RNA transport, and mRNA surveillance [53]. In another study, three-month-old drought-tolerant ('RB867515') and drought-sensitive ('RB855536') sugarcane plants were subjected to 2, 4, 6, or 8 days of drought stress in order to identify the miRNAs involved in regulating the drought stress response.…”