Physiological, biochemical, and molecular responses of wheat seedlings to salinity and plant-derived smoke

Çatav, Şükrü Serter; Surgun-Acar, Yonca; Zemheri‐Navruz, Fahriye

doi:10.1016/j.sajb.2021.02.011

Cited by 12 publications

(6 citation statements)

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“…Seeds under salt stress often show reduced germination and vigour and even death in severe cases, and such phenomena are mainly caused by water stress and ion imbalance ( Çatav et al., 2021 ). Figure 8A reflects that the germination curve of wheat seeds showed a decreasing trend with increasing salt concentration, and the germination rate was CK > S1 > S2 in descending order, which is consistent with the results of previous studies ( Zhu et al., 2021 ).…”

Section: Resultsmentioning

confidence: 99%

WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour

Han

Liu

et al. 2022

Front. Plant Sci.

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Salt stress is one of the major environmental stress factors that affect and limit wheat production worldwide. Therefore, properly evaluating wheat genotypes during the germination stage could be one of the effective ways to improve yield. Currently, phenotypic identification platforms are widely used in the seed breeding process, which can improve the speed of detection compared with traditional methods. We developed the Wheat Seed Vigour Assessment System (WSVAS), which enables rapid and accurate detection of wheat seed germination using the lightweight convolutional neural network YOLOv4. The WSVAS system can automatically acquire, process and analyse image data of wheat varieties to evaluate the response of wheat seeds to salt stress under controlled environments. The WSVAS image acquisition system was set up to continuously acquire images of seeds of four wheat varieties under three types of salt stress. In this paper, we verified the accuracy of WSVAS by comparing manual scoring. The cumulative germination curves of wheat seeds of four genotypes under three salt stresses were also investigated. In this study, we compared three models, VGG16 + Faster R-CNN, ResNet50 + Faster R-CNN and YOLOv4. We found that YOLOv4 was the best model for wheat seed germination target detection, and the results showed that the model achieved an average detection accuracy (mAP) of 97.59%, a recall rate (Recall) of 97.35% and the detection speed was up to 6.82 FPS. This proved that the model could effectively detect the number of germinating seeds in wheat. In addition, the germination rate and germination index of the two indicators were highly correlated with germination vigour, indicating significant differences in salt tolerance amongst wheat varieties. WSVAS can quantify plant stress caused by salt stress and provides a powerful tool for salt-tolerant wheat breeding.

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Section: Resultsmentioning

confidence: 99%

WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour

Han

Liu

et al. 2022

Front. Plant Sci.

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“…After the suspension was removed, normal saline was added into the residues to obtain the homogenates (10.0%, w/v). After the homogenates were centrifuged at 2,000 rpm min -1 at 4 °C for 15min, supernatant was obtained to conduct T-SOD analysis (hydroxylamine method) and MDA analysis (TBA method), and finally were measured by microplate system ( Çatav et al., 2021 ; Zhang et al., 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Single and composite damage mechanisms of soil polyethylene/polyvinyl chloride microplastics to the photosynthetic performance of soybean (Glycine max [L.] merr.)

Song

et al. 2023

Front. Plant Sci.

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IntroductionAdverse impacts of soil microplastics (MPs, diameter<5 mm) on vegetative growth and crop production have been widely reported, however, the single and composite damage mechanisms of polyethylene (PE) /polyvinyl chloride (PVC) microplastics (MPs) induced photosynthesis inhibition are still rarely known.MethodsIn this study, two widely distributed MPs, PE and PVC, were added to soils at a dose of 7% (dry soil) to examine the single and composite effects of PE-MPs and PVC-MPs on the photosynthetic performance of soybean.ResultsResults showed PE-MPs, PVC-MPs and the combination of these two contaminants increased malondialdehyde (MDA) content by 21.8-97.9%, while decreased net photosynthesis rate (Pn) by 11.5-22.4% compared to those in non-stressed plants, PVC MPs caused the most severe oxidative stress, while MPs stress resulted in Pn reduction caused by non-stomatal restriction. The reason for this is the single and composite MPs stress resulted in a 6% to 23% reduction in soybean PSII activity RCs reaction centers, along with negative effects on soybean PSII energy uptake, capture, transport, and dissipation. The presence of K-band and L-band also represents an imbalance in the number of electrons on the donor and acceptor side of PSII and a decrease in PSII energy transfer. Similarly, PVC single stress caused greater effects on soybean chloroplast PSII than PE single stress and combined stresses.DiscussionPE and PVC microplastic stress led to oxidative stress in soybean, which affected the structure and function of photosynthetic PSII in soybean, ultimately leading to a decrease in net photosynthetic rate in soybean.

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“…These studies suggest that the application of plant-derived smoke or KAR to plants under salt stress further increases proline accumulation while decreasing the MDA accumulation and production of H2O2 (Sharma et al 2012;Vardhini & Anjum 2015). In addition, the effects of plant-derived smoke and KARs on the transcription factors and various antioxidant enzymes (CAT, SOD, and GPX) against salinity stress have been investigated (Sharifi & Shirani Bidabadi (2020); Çatav et al 2021;Hayat et al 2022). Previous research has indicated that plant-derived smoke and KAR increase the expression of CAT, SOD, and GPX genes in plants under salt stress (Shah et al 2020;Sharifi & Shirani Bidabadi 2020;Çatav et al 2021;Shah et al 2021;Hayat et al 2022).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the effects of plant-derived smoke and KARs on the transcription factors and various antioxidant enzymes (CAT, SOD, and GPX) against salinity stress have been investigated (Sharifi & Shirani Bidabadi (2020); Çatav et al 2021;Hayat et al 2022). Previous research has indicated that plant-derived smoke and KAR increase the expression of CAT, SOD, and GPX genes in plants under salt stress (Shah et al 2020;Sharifi & Shirani Bidabadi 2020;Çatav et al 2021;Shah et al 2021;Hayat et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Effects of plant-derived smoke, karrikin, and salinity stress on Prunus armeniaca cv. Şalak seeds and seedlings: A morphological, biochemical, and molecular approach

KEMEÇ HÜRKAN,

AKI

2023

J Agr Sci-Tarim Bili

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There is great interest in developing strategies to reduce the detrimental effects of salinity stress to improve plant growth and yield. The effects of plant-derived smoke on seed germination and plant growth, depending on concentration and time, are widely known. Although it has been suggested that plant-derived smoke and Karrikin (KAR1) tolerate the harmful effects of abiotic stress in plants, there is a lack of comprehensive information on biochemical and molecular changes related to KAR1 and smoke water (SW) in plants. Here we extensively compared the effects of SW and KAR1 on seed germination and morphology. Here we comprehensively compared the effects of SW and KAR1 on seed germination and morphological, biochemical, and molecular changes observable in the examined seeds. Moreover, we also showed that it regulated the expression of some genes encoding antioxidant enzymes in apricot seedlings (Prunus armeniaca L.) exposed to salinity stress (100 mM NaCl). Morphologically, we observed more adverse effects with decreased concentration in the SW treatment and increased concentration in the KAR1 treatment. The data revealed that SW (1:1000 v/v) and KAR1 (1µM) increased the expression levels of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPX) genes in the samples taken from the apricot seedlings treated with salt at hours 3, 6 and 9. This increase varies in SW and KAR1 depending on time. When the biochemical results were examined, it was seen that the application of SW and KAR1 to the seedlings under salinity stress led to a significant decrease in the thiobarbituric acid reactive substances (TBARS) content. We can assert that SW is more effective than KAR1 on TBARS content. Morphological, molecular, and biochemical results revealed enhanced germination, growth, gene expression, and TBARS content in apricot seeds and seedlings exposed to SW and KAR1. This data may be applicable to more comprehensive trials.

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Physiological, biochemical, and molecular responses of wheat seedlings to salinity and plant-derived smoke

Cited by 12 publications

References 81 publications

WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour

WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour

Single and composite damage mechanisms of soil polyethylene/polyvinyl chloride microplastics to the photosynthetic performance of soybean (Glycine max [L.] merr.)

Effects of plant-derived smoke, karrikin, and salinity stress on Prunus armeniaca cv. Şalak seeds and seedlings: A morphological, biochemical, and molecular approach

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