Physiological attributes of microbial BTEX degradation in oxygen-limited environments.

Olsen, Ronald H.; Mikesell, Mark D.; Kukor, Jerome J.; Byrne, Armando M.

doi:10.1289/ehp.95103s449

Cited by 13 publications

(13 citation statements)

References 7 publications

(3 reference statements)

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“…In this context, Geyer et al (2005) demonstrated recently ongoing benzene and toluene metabolization processes under aquifer conditions in a monitoring well located at the field site by the use of 13 C-labelled in situ microcosms, thus supporting our hypothesis and results. It is well known from literature that bacteria with specific physiological traits are active degraders of BTEX under hypoxic (dissolved oxygen is <2 mg l −1 ) aquifer conditions (Olsen et al 1995;Kukor and Olsen 1996). It was also proposed that the microbial degradation of aromatic hydrocarbons is performed under mixed electron acceptor conditions, associated with the reduction of nitrate (Wilson and Bouwer 1997).…”

Section: Detection Of Catechol Dioxygenase Genes and Cultivable Btex-mentioning

confidence: 99%

Bacterial Diversity and Aerobic Biodegradation Potential in a BTEX-Contaminated Aquifer

Alfreider

Vogt

2007

Water Air Soil Pollut

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In order to gain insight into the community structure and the biodegradative potential of bacteria residing in a BTEX-contaminated field site, with benzene as main pollutant, an investigation strategy with a variety of cultivation-independent and -dependent approaches was undertaken. Based on 16S ribosomal DNA sequence analysis, sampling stations in the center of the pollution plume were found to be dominated by a bacterial consortium affiliated with various members of the class of Proteobacteria and Firmicutes, including different sporulating and nonsporulating sulfate-reducing bacteria and members of the genus Geobacter. Non-polluted samples retrieved from outside the plume revealed several phylotypes which were also observed in the centre of the plume.Bacterial sequences retrieved from the fringe of the plume were dominated by several genera of beta Proteobacteria subclass which were not associated with other phylotypes obtained in this study. All sampling stations were tested positive for catechol 2,3-dioxygenase genes, indicating the presence of microorganisms with the genetic potential degrading aromatic compounds via the meta-cleavage pathway.The groundwater system at the down-gradient edge of the plume was characterized, in contrast to all other sampling stations in the center and outside the plume, by high numbers of cultivable bacteria and cultivable aerobic benzene and toluene degraders. These findings are also in accordance with the investigations based on fluorescent in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes, suggesting the presence of an actively benzene degrading bacterial community of beta Proteobacteria at the fringe of the pollution plume.

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Section: Detection Of Catechol Dioxygenase Genes and Cultivable Btex-mentioning

confidence: 99%

Bacterial Diversity and Aerobic Biodegradation Potential in a BTEX-Contaminated Aquifer

Alfreider

Vogt

2007

Water Air Soil Pollut

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“…The Pseudomonas putida strains have been examined in a considerable number of studies, some of which we have chosen to list here: P. putida (Shim and Yang 1999, 2006Shim et al , 2005; P. putida 39/D Ziffer et al 1973); P. putida biotype A (Mallakin and Ward 1996); P. putida BP18 (Baldwin et al 2000); P. putida F1 (Olsen et al 1994a(Olsen et al , b, 1995Reardon et al 2000Reardon et al , 2002Yu et al 2001a, b;Gunaseelan and Cowan 2003); P. putida O1 (Oh and Bartha 1997;Oh and Choi 1997); P. putida ML2 (Tan and Mason 1990); P. putida mt2 (Gunaseelan and Cowan 2003), and so on. However, not only P. putida has been proven to be a popular research strain of Pseudomonas, but other strains of Pseudomonas have also been studied in benzene degradation, such as: P. aeruginosa (Marr and Stone 1961;Kim et al 2003); P. alcaligenes (Oh and Choi 1997); P. fluorescens (Shim and Yang 1999, 2006Shim et al , 2005; P. fragi B1 (Chang et al 1993); P. maltophilia (Mallakin and Ward 1996); P. mendocina KR (Gunaseelan and Cowan 2003); P. paucimobilis Q1 (Furukawa et al 1983); P. pickettii PKO1 (Olsen et al 1995); P. stutzeri (Oh and Bartha 1997;Oh and Choi 1997); P. testosteron (Mallakin and Ward 1996); Pseudomonas sp. (Shirai 1986;Jean et al 2002Jean et al , 2008; Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

“…ATCC 55595 (Collins and Daugulis 1999a, b); Pseudomonas sp. CFS215 (Alvarez and Vogel 1991;Olsen et al 1995); Pseudomonas sp. G4 (Olsen et al 1994a(Olsen et al , b, 1995; Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of benzene by pure and mixed cultures of Bacillus spp.

Liu

Maity

Jean

et al. 2010

World J Microbiol Biotechnol

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Benzene has a wide range of industrial applications, but it is also a major source of environmental pollution. The most eco-friendly/cost-effective method of remediation is biodegradation. In the present study, we used a variety of microbial strains in different combinations on a selection of substrate concentrations to determine the most effective degradation processes. Bacterial strains of pure culture (L 4 , N 3 , and N 6 ) were isolated from oil sludge in both Luria-Bertani buffer (LB) and nutrient broth media, and identified by 16S-rRNA analysis (C98% similarity). The degradation experiments were performed using different combinations of bacterial strains

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“…These bacteria exhibit enhanced rates of phenol biodegradation in the presence of NO 3 À as O 2 that would have been used for respiration is available for phenol biodegradation (Olsen et al, 1995;Baek et al, 2001Baek et al, , 2003. Pseudomonas spp.…”

Section: Phenol Biodegradationmentioning

confidence: 99%

Dynamic changes in microbial community structure and function in phenol-degrading microcosms inoculated with cells from a contaminated aquifer

Elliott

Scholes

Thornton

et al. 2010

FEMS Microbiology Ecology

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Contamination of aquifers by organic pollutants threatens groundwater supplies and the environment. In situ biodegradation of organic pollutants by microbial communities is important for the remediation of contaminated sites, but our understanding of the relationship between microbial development and pollutant biodegradation is poor. A particular challenge is understanding the in situ status of microorganisms attached to solid surfaces, but not accessible via conventional sampling of groundwater. We have developed novel flow-through microcosms and examined dynamic changes in microbial community structure and function in a phenol-degrading system. Inoculation of these microcosms with a complex microbial community from a plume in a phenol-contaminated aquifer led to the initial establishment of a population dominated by a few species, most attached to the solid substratum. Initially, phenol biodegradation was incomplete, but as the microbial community structure became more complex, phenol biodegradation was more extensive and complete. These results were replicated between independent microcosms, indicating a deterministic succession of species. This work demonstrates the importance of examining community dynamics when assessing the potential for microbial biodegradation of organic pollutants. It provides a novel system in which such measurements can be made readily and reproducibly to study the temporal development and spatial succession of microbial communities during biodegradation of organic pollutants at interfaces within such environments.

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Physiological attributes of microbial BTEX degradation in oxygen-limited environments.

Cited by 13 publications

References 7 publications

Bacterial Diversity and Aerobic Biodegradation Potential in a BTEX-Contaminated Aquifer

Bacterial Diversity and Aerobic Biodegradation Potential in a BTEX-Contaminated Aquifer

Biodegradation of benzene by pure and mixed cultures of Bacillus spp.

Dynamic changes in microbial community structure and function in phenol-degrading microcosms inoculated with cells from a contaminated aquifer

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