Physiological and Transcriptomics Analyses Reveal that Ascophyllum nodosum Extracts Induce Salinity Tolerance in Arabidopsis by Regulating the Expression of Stress Responsive Genes

Jithesh, M. N.; Shukla, Pushp Sheel; Kant, Pragya; Joshi, Jyoti; Critchley, Alan T.; Prithiviraj, Balakrishnan

doi:10.1007/s00344-018-9861-4

Cited by 65 publications

(67 citation statements)

References 84 publications

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“…Similarly, the high expression of at-miR827 in the presence of NaCl was reduced by the ANE treatment, also increasing the expression of its target AtNLA gene under these circumstances. These studies demonstrated that the alleviation of salt stress when different ANEs are applied results from the contribution of a multitude of different stress-related genes (Jithesh et al 2018) and involves phosphate homeostasis (Shukla et al 2018).…”

Section: Molecular Analysis Of Ane-induced Salinity Stress Tolerance mentioning

confidence: 97%

“…As such, At1g62760, encoding a putative pectin methylesterase inhibitor, was identified as a novel negative regulator of salt tolerance (Jithesh et al 2012). In a subsequent study, 2-week-old Arabidopsis Col-0 plants subjected to 100 and 150 mM NaCl for 24 h were treated with a 1 g L −1 equivalent of a methanolic ANE fraction (MEA) (Jithesh et al 2018). As compared with the control, the plants treated with 150 mM NaCl and MEA had a larger leaf area (+37.3%), an increased plant height (+33%), more leaves (+33%), and a higher biomass (+57%), whereas the increases were slightly lower in the plants treated with 100 mM NaCl and MEA.…”

Section: Molecular Analysis Of Ane-induced Salinity Stress Tolerance mentioning

confidence: 99%

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Toward the molecular understanding of the action mechanism of Ascophyllum nodosum extracts on plants

et al. 2019

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The importance of biostimulants, defined as plant growth-promoting agents that differ notably from fertilizers, is increasing steadily because of their potential contribution to a worldwide strategy for securing food production without burdening the environment. Based on folkloric evidence and ethnographic studies, seaweeds have been useful for diverse human activities through time, including medicine and agriculture. Currently, seaweed extracts, especially those derived from the common brown alga Ascophyllum nodosum, represent an interesting category of biostimulants. Although A. nodosum extracts (abbreviated ANEs) are readily used because of their capacity to improve plant growth and to mitigate abiotic and biotic stresses, fundamental insights into how these positive responses are accomplished are still fragmentary. Generally, the effects of ANEs on plants have been attributed to their hormonal content, their micronutrient value, and/or the presence of alga-specific polysaccharides, betaines, polyamines, and phenolic compounds that would, alone or in concert, bring about the observed phenotypic effects. However, only a few of these hypotheses have been validated at the molecular level. Transcriptomics and metabolomics are now emerging as tools to dissect the action mechanisms exerted by ANEs. Here, we provide an overview of the available in planta molecular data that shed light on the pathways modulated by ANEs that promote plant growth and render plants more resilient to diverse stresses, paving the way toward the elucidation of the modus operandi of these extracts.

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Section: Molecular Analysis Of Ane-induced Salinity Stress Tolerance mentioning

confidence: 97%

Section: Molecular Analysis Of Ane-induced Salinity Stress Tolerance mentioning

confidence: 99%

Toward the molecular understanding of the action mechanism of Ascophyllum nodosum extracts on plants

et al. 2019

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“…In fact, quantitative RT-PCR analysis of genes directly related to stress revealed that for instance, GmCYP707A1a and GmCYP707A3b, two genes involved in regulating ABA biosynthesis during dehydration and hydration cycles, were overexpressed due to SE application; and such response was not recorded before stress, as gene expression remained unchanged in both control and SE treated plants. On another note, Jithesh et al [92] experienced the effect of an Ascophyllum nodosum extract on Arabidopsis thaliana under salinity conditions using transcriptomics and physiological analyses. The plants treated with an ethyl acetate sub-fraction of Ascophyllum nodosum showed an up-regulation of 184 and 257 genes at the first and fifth days, respectively, and a down regulation of 91 and 262 genes at day 1 and 5, respectively.…”

Section: Mechanism Of Action Under Abiotic Stressmentioning

confidence: 99%

Trends in Seaweed Extract Based Biostimulants: Manufacturing Process and Beneficial Effect on Soil-Plant Systems

Boukhari

Barakate

Bouhia

et al. 2020

Plants

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The time when plant biostimulants were considered as “snake oil” is erstwhile and the skepticism regarding their agricultural benefits has significantly faded, as solid scientific evidences of their positive effects are continuously provided. Currently plant biostimulants are considered as a full-fledged class of agri-inputs and highly attractive business opportunity for major actors of the agroindustry. As the dominant category of the biostimulant segment, seaweed extracts were key in this growing renown. They are widely known as substances with the function of mitigating abiotic stress and enhancing plant productivity. Seaweed extracts are derived from the extraction of several macroalgae species, which depending on the extraction methodology lead to the production of complex mixtures of biologically active compounds. Consequently, plant responses are often inconsistent, and precisely deciphering the involved mechanism of action remains highly intricate. Recently, scientists all over the world have been interested to exploring hidden mechanism of action of these resources through the employment of multidisciplinary and high-throughput approaches, combining plant physiology, molecular biology, agronomy, and multi-omics techniques. The aim of this review is to provide fresh insights into the concept of seaweed extract (SE), through addressing the subject in newfangled standpoints based on current scientific knowledge, and taking into consideration both academic and industrial claims in concomitance with market’s requirements. The crucial extraction process as well as the effect of such products on nutrient uptake and their role in abiotic and biotic stress tolerance are scrutinized with emphasizing the involved mechanisms at the metabolic and genetic level. Additionally, some often overlooked and indirect effects of seaweed extracts, such as their influence on plant microbiome are discussed. Finally, the plausible impact of the recently approved plant biostimulant regulation on seaweed extract industry is addressed.

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“…A. nodosum biomass is used to produce one of the most commonly studied seaweed-based biostimulants [8][9][10]. Commercial extracts of the brown alga have been reported to enhance plant growth as well as to promote the growth of beneficial soil microbes and induce plant resistance against biotic and abiotic stresses [9,11]. Ascophyllum nodosum extracts (ANE) have been reported to suppress disease incidence and the growth of various pathogens including Alternaria cucumerinum, Didymella applanata, Fusarium oxysporum, Botrytis cinerea [12,13], Colletorichum lagenarium [14], Phytophthora capsica [15] and Verticillium sp.…”

Section: Introductionmentioning

confidence: 99%

Combined application of Ascophyllum nodosum extract and chitosan synergistically activates host-defense of peas against powdery mildew

et al. 2020

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Background: Powdery mildew (PM) is an important disease of pea that reduce yield. Ascophyllum nodosum extract (ANE) and chitosan (CHT) are biostimulants used to improve plant health. Efficacy of ANE and CHT was assessed individually and in combination against pea powdery mildew. Results: Combined applications of ANE and CHT had a significant inhibitory effect on pathogen development and it reduced disease severity to 35%, as compared to control (90.5%). The combination of ANE and CHT enhanced the activity of plant defense enzymes; phenylalanine ammonia lyases (PAL), peroxidase (PO) and production of reactive oxygen species (ROS) and hydrogen peroxide (H 2 O 2). Further, the treatment increased the expression of a number of plant defense genes in jasmonic acid (JA) signaling pathway such as LOX1 and COI and salicylic acid (SA)mediated signaling pathway such as NPR1 and PR1. Other genes involved in defense mechanisms like NADPH oxidase and C4H were also upregulated by the combination treatment. Conclusion: The combination of ANE and CHT suppresses pea powdery mildew largely by modulating JA and SAmediated signaling pathways.

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Physiological and Transcriptomics Analyses Reveal that Ascophyllum nodosum Extracts Induce Salinity Tolerance in Arabidopsis by Regulating the Expression of Stress Responsive Genes

Cited by 65 publications

References 84 publications

Toward the molecular understanding of the action mechanism of Ascophyllum nodosum extracts on plants

Toward the molecular understanding of the action mechanism of Ascophyllum nodosum extracts on plants

Trends in Seaweed Extract Based Biostimulants: Manufacturing Process and Beneficial Effect on Soil-Plant Systems

Combined application of Ascophyllum nodosum extract and chitosan synergistically activates host-defense of peas against powdery mildew

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