Physiological and Transcriptomic Analysis provide Molecular Insight into 24-Epibrassinolide mediated Cr(VI)-Toxicity Tolerance in Pepper Plants

Mumtaz, Muhammad Ali; Hao, Yuanyuan; Mehmood, Sajid; Shu, Huangying; Zhang, He; Jin, Weiheng; Chen, Chuhao; Lin, Li; Altaf, Ahsan; Wang, Zhiwei

doi:10.1016/j.envpol.2022.119375

Cited by 36 publications

(21 citation statements)

References 58 publications

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“…Images of autofluorescence emitted at 650–700 nm (chlorophylls) or 500–550 nm (carotenoids) were obtained after excitation with the 488 nm ray line of an argon laser. To observe the chlorophyll and carotenoid (lycopene) contents, a TCS SP2 laser confocal microscope (Leica, Heidelberg, Germany) was used, as previously reported by Mumtaz et al [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Melatonin Affects the Photosynthetic Performance of Pepper (Capsicum annuum L.) Seedlings under Cold Stress

et al. 2022

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Photosynthesis is an important plant metabolic mechanism that improves carbon absorption and crop yield. Photosynthetic efficiency is greatly hampered by cold stress (CS). Melatonin (ME) is a new plant growth regulator that regulates a wide range of abiotic stress responses. However, the molecular mechanism of ME-mediated photosynthetic regulation in cold-stressed plants is not well understood. Our findings suggest that under low-temperature stress (15/5 °C for 7 days), spraying the plant with ME (200 µM) enhanced gas exchange characteristics and the photosynthetic pigment content of pepper seedlings, as well as upregulated their biosynthetic gene expression. Melatonin increased the activity of photosynthetic enzymes (Rubisco and fructose-1, 6-bisphosphatase) while also enhancing starch, sucrose, soluble sugar, and glucose content under CS conditions. Low-temperature stress significantly decreased the photochemical activity of photosystem II (PSII) and photosystem I (PSI), specifically their maximum quantum efficiency PSII (Fv/Fm) and PSI (Pm). In contrast, ME treatment improved the photochemical activity of PSII and PSI. Furthermore, CS dramatically reduced the actual PSII efficiency (ΦPSII), electron transport rate (ETR) and photochemical quenching coefficient (qP), while enhancing nonphotochemical quenching (NPQ); however, ME treatment substantially mitigated the effects of CS. Our results clearly show the probable function of ME treatment in mitigating the effects of CS by maintaining photosynthetic performance, which might be beneficial when screening genotypes for CS tolerance.

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Section: Methodsmentioning

confidence: 99%

Melatonin Affects the Photosynthetic Performance of Pepper (Capsicum annuum L.) Seedlings under Cold Stress

et al. 2022

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“…An S-4800 microscope (Hitachi Led., Tokyo, Japan, Model TM-1000) was used to record the SEM observations. For observing the contents of chlorophyll and carotenoid, TCS SP2 laser confocal microscope (Leica, Germany) was used, as previously reported by Mumtaz et al (2022) .…”

Section: Methodsmentioning

confidence: 99%

Physiological and Biochemical Responses of Pepper (Capsicum annuum L.) Seedlings to Nickel Toxicity

Altaf

Hao

et al. 2022

Front. Plant Sci.

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Globally, heavy metal pollution of soil has remained a problem for food security and human health, having a significant impact on crop productivity. In agricultural environments, nickel (Ni) is becoming a hazardous element. The present study was performed to characterize the toxicity symptoms of Ni in pepper seedlings exposed to different concentrations of Ni. Four-week-old pepper seedlings were grown under hydroponic conditions using seven Ni concentrations (0, 10, 20, 30, 50, 75, and 100 mg L–1 NiCl2. 6H2O). The Ni toxicity showed symptoms, such as chlorosis of young leaves. Excess Ni reduced growth and biomass production, root morphology, gas exchange elements, pigment molecules, and photosystem function. The growth tolerance index (GTI) was reduced by 88-, 75-, 60-, 45-, 30-, and 19% in plants against 10, 20, 30, 50, 75, and 100 mg L–1 Ni, respectively. Higher Ni concentrations enhanced antioxidant enzyme activity, ROS accumulation, membrane integrity [malondialdehyde (MDA) and electrolyte leakage (EL)], and metabolites (proline, soluble sugars, total phenols, and flavonoids) in pepper leaves. Furthermore, increased Ni supply enhanced the Ni content in pepper’s leaves and roots, but declined nitrogen (N), potassium (K), and phosphorus (P) levels dramatically. The translocation of Ni from root to shoot increased from 0.339 to 0.715 after being treated with 10–100 mg L–1 Ni. The uptake of Ni in roots was reported to be higher than that in shoots. Generally, all Ni levels had a detrimental impact on enzyme activity and led to cell death in pepper seedlings. However, the present investigation revealed that Ni ≥ 30 mg L–1 lead to a deleterious impact on pepper seedlings. In the future, research is needed to further explore the mechanism and gene expression involved in cell death caused by Ni toxicity in pepper plants.

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“…Seed germination, cell division, senescence, stomatal opening, seedling development, root growth, and vascular differentiation are all aided by BRs (Ullah et al, 2018;Chaudhuri et al, 2022). Different morphological and physiological processes in horticultural crops are effectively controlled by BRs (Mumtaz et al, 2022). Currently, it is clarified that BRs may confer significant tolerance in plants towards many abiotic stressors such as salt, water logging, drought, metals, and high and low temperatures (Ahammed et al, 2020;Kour et al, 2021).…”

Section: Brassinosteroidsmentioning

confidence: 99%

Phytohormones regulate the abiotic stress: An overview of physiological, biochemical, and molecular responses in horticultural crops

et al. 2023

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Recent changing patterns of global climate have turned out to be a severe hazard to the horticulture crops production. A wide range of biotic and abiotic stresses often affect plants due to their sessile nature. Horticultural crop losses are mainly caused by abiotic factors such as drought, salt, heat, cold, floods, and ultraviolet radiation. For coping up with these adversities, well-developed mechanisms have been evolved in plants, which play a role in perceiving stress signals and enabling optimal growth responses. Interestingly, the use of phytohormones for suppressing the impact of abiotic stress has gained much attention in recent decades. For circumvention of stress at various levels, including physiological, molecular, as well as biochemical, a sophisticated mechanism is reported to be provided by the phytohormones, thus labeling these phytohormones a significant role in plant growth and development. Phytohormones can improves tolerance against abiotic stresses by increasing seed germination, seedling growth, leaf photosynthesis, root growth, and antioxidant enzymes and reducing the accumulation of reactive oxygen species, malonaldehyde, and electrolyte leakage. Recent discoveries highlight the significant role of a variety of phytohormones including melatonin (MEL), Gamma-aminobutyric acid (GABA), jasmonic acid (JA), salicylic acid (SA), brassinosteroids (BRs), and strigolactones (SLs) in abiotic stress tolerance enhancement of horticultural plants. Thus, current review is aimed to summarize the developmental concepts regarding role of phytohormones in abiotic-stress mitigation, mainly in horticultural crops, along with the description of recent studies which identified the role of different phytohormones in stressed environments. Hence, such a review will help in paving the path for sustainable agriculture growth via involvement of phytohormones in enhancement of abiotic stress tolerance of horticultural crops.

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Physiological and Transcriptomic Analysis provide Molecular Insight into 24-Epibrassinolide mediated Cr(VI)-Toxicity Tolerance in Pepper Plants

Cited by 36 publications

References 58 publications

Melatonin Affects the Photosynthetic Performance of Pepper (Capsicum annuum L.) Seedlings under Cold Stress

Melatonin Affects the Photosynthetic Performance of Pepper (Capsicum annuum L.) Seedlings under Cold Stress

Physiological and Biochemical Responses of Pepper (Capsicum annuum L.) Seedlings to Nickel Toxicity

Phytohormones regulate the abiotic stress: An overview of physiological, biochemical, and molecular responses in horticultural crops

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