Physiological and Transcriptome Analysis of Sugar Beet Reveals Different Mechanisms of Response to Neutral Salt and Alkaline Salt Stresses

Geng, Gui; Li, Renren; Stevanato, Piergiorgio; Lv, Chunhua; Liu, Zhengyu; Yu, Lijie; Wang, Yuguang

doi:10.3389/fpls.2020.571864

Cited by 28 publications

(22 citation statements)

References 42 publications

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“…Real-time PCR was performed in a Two-color Real-time PCR Detection System (Bio-Rad, Hercules, CA, USA) with SYBR Green PCR Mix (TaKaRa, Dalian, China). The 18S rRNA and Actin were chosen as internal controls in sugar beet and Arabidopsis , respectively [ 36 ]. PCR reaction was carried out in 10 µL volumes using the following amplification protocol: 94 °C for 4 min; 94 °C for 30 s, 53 °C for 20 s, and 72 °C for 70 s; and 72 °C for 4 min and 45 cycles.…”

Section: Methodsmentioning

confidence: 99%

Functional Characterization of a Sugar Beet BvbHLH93 Transcription Factor in Salt Stress Tolerance

Wang

Tian

et al. 2021

IJMS

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The basic/helix–loop–helix (bHLH) transcription factor (TF) plays an important role for plant growth, development, and stress responses. Previously, proteomics of NaCl treated sugar beet leaves revealed that a bHLH TF, BvbHLH93, was significantly increased under salt stress. The BvbHLH93 protein localized in the nucleus and exhibited activation activity. The expression of BvbHLH93 was significantly up-regulated in roots and leaves by salt stress, and the highest expression level in roots and leaves was 24 and 48 h after salt stress, respectively. Furthermore, constitutive expression of BvbHLH93 conferred enhanced salt tolerance in Arabidopsis, as indicated by longer roots and higher content of chlorophyll than wild type. Additionally, the ectopic expression lines accumulated less Na+ and MDA, but more K+ than the WT. Overexpression of the BvBHLH93 enhanced the activities of antioxidant enzymes by positively regulating the expression of antioxidant genes SOD and POD. Compared to WT, the overexpression plants also had low expression levels of RbohD and RbohF, which are involved in reactive oxygen species (ROS) production. These results suggest that BvbHLH93 plays a key role in enhancing salt stress tolerance by enhancing antioxidant enzymes and decreasing ROS generation.

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Section: Methodsmentioning

confidence: 99%

Functional Characterization of a Sugar Beet BvbHLH93 Transcription Factor in Salt Stress Tolerance

Wang

Tian

et al. 2021

IJMS

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“…Malondialdehyde (MDA) content was determined by the thiobarbituric acid (TBA) reaction using the method described by Chołuj et al (2014) . Soluble sugar and proline content were determined using the method given in Geng et al (2020) . The total nitrogen content of sugar beet seedlings was measured using the method described by Wang et al (2017a) .…”

Section: Methodsmentioning

confidence: 99%

Physiological and Proteomic Analysis of Different Molecular Mechanisms of Sugar Beet Response to Acidic and Alkaline pH Environment

et al. 2021

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Soil pH is a major constraint to crop plant growth and production. Limited data are available on sugar beet growth status under different pH conditions. In this study, we analyzed the growth status and phenotype of sugar beet under pH 5, pH 7.5, and pH 9.5. It was found that the growth of sugar beet was best at pH 9.5 and worst at pH 5. The activities of superoxide dismutase (SOD) and peroxidase (POD) in leaves and roots increased as pH decreased from 9.5 to 5. Moreover, compared with pH 9.5, the levels of soluble sugar and proline in leaves increased significantly at pH 5. To explore the mechanisms of sugar beet response to different soil pH environments, we hypothesized that proteins play an important role in plant response to acidic and alkaline pH environment. Thus, the proteome changes in sugar beet modulated by pH treatment were accessed by TMT-based quantitative proteomic analysis. A total of three groups of differentially expressed proteins (DEPs) (pH 5 vs. pH 7.5, pH 9.5 vs. pH7.5 and pH 5 vs. pH 9.5) were identified in the leaves and roots of sugar beet. Several key proteins related to the difference of sugar beet response to acid (pH 5) and alkaline (pH 9.5) and involved in response to acid stress were detected and discussed. Moreover, based on proteomics results, QRT-PCR analysis confirmed that expression levels of three N transporters (NTR1, NRT2.1, and NRT2.5) in roots were relatively high under alkaline conditions (pH 9.5) compared with pH 5 or pH 7.5. The total nitrogen content of pH 9.5 in sugar beet was significantly higher than that of pH 7.5 and pH 5. These studies increase our understanding of the molecular mechanism of sugar beet response to different pH environments.

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“…Salt affected soils contain excessive soluble salts and exchangeable sodium on the surface or in the rhizosphere, which is associated with two main challenges to plants: osmotic stress and ionic stress. osmotic stress can due to the excess solutes outside the roots that reduce the ability of plants to extract soil water, and ionic stress which is usually caused by excessive influx of Na + into the plant and lead to imbalanced metabolism, chlorosis, impaired photosynthesis, nutrient imbalance and yield loss (Deinlein et al, 2014;Hasanuzzaman et al, 2014;Geng et al, 2020). Growth inhibition is the most common physiological response of plants to salt, when salt accumulates above a certain concentration, it stops the plants from absorbing water and results in osmotic stress (Wu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Two Contrasting Chinese Cabbage (Brassica rapa L.) Genotypes Reveals That Ion Homeostasis Is a Crucial Biological Pathway Involved in the Rapid Adaptive Response to Salt Stress

Zhang

Chen

et al. 2021

Front. Plant Sci.

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Salt is the most important limiting factor in plant yield and quality. Different Chinese cabbage cultivars appeared different salt tolerances, but there are few studies attempting to elucidate the mechanism underlying this phenomenon. In this study, 100 mmol L–1 NaCl was found to be the most suitable treatment concentration according to a sprouting bag test of 39 Chinese cabbage cultivars, and through comprehensive comparison and analysis, the relative values of fresh weight and electrolyte leakage in leaves proved to be convenient indicators for the identification of salt tolerance in Chinese cabbage. We analyzed the physiological responses of Qinghua45 (salt-tolerant) and Biyuchunhua (salt-sensitive) in terms of the growth indexes, ion homeostasis and Photosynthesis, the results indicated that Qinghua45 could ensure osmotic regulation, ion homeostasis and photosynthesis under salt stress. Next, we compared the transcriptome dynamics of the two cultivars. Overall, 2,859 differentially expressed genes (DEGs) were identified, and the number of DEGs in Qinghua45 was significantly less than that in Biyuchunhua. VDAC promoted the release of Ca2+, which indirectly promoted the transport of Na+ to vacuoles through the SOS2 pathway. Cation/H (+) antiporter 17 and V-H + -ATPase improve the exchange of Na+ and H+ and maintain Na+ in the vacuoles, thereby reducing the injury affected by salt stress. Increases in galactinol synthase and soluble protein synthesis helped relieve osmotic stress caused by salt, together, they regulated the Na+ content and chlorophyll biosynthesis of the plant and enabled the plant to adapt to salt stress over time.

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Physiological and Transcriptome Analysis of Sugar Beet Reveals Different Mechanisms of Response to Neutral Salt and Alkaline Salt Stresses

Cited by 28 publications

References 42 publications

Functional Characterization of a Sugar Beet BvbHLH93 Transcription Factor in Salt Stress Tolerance

Functional Characterization of a Sugar Beet BvbHLH93 Transcription Factor in Salt Stress Tolerance

Physiological and Proteomic Analysis of Different Molecular Mechanisms of Sugar Beet Response to Acidic and Alkaline pH Environment

Comparative Transcriptome Analysis of Two Contrasting Chinese Cabbage (Brassica rapa L.) Genotypes Reveals That Ion Homeostasis Is a Crucial Biological Pathway Involved in the Rapid Adaptive Response to Salt Stress

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