Physiological and transcriptome analyses to infer regulatory networks in flowering transition of&lt;i&gt; Rosa rugosa&lt;/i&gt;

Wang, Xiaobin; Zhao, Fei; Wu, Qikui; Xing, Shutang; Yu, Yunyan; Qi, Shihua

doi:10.48130/opr-2023-0004

Cited by 2 publications

(2 citation statements)

References 53 publications

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“…They observed signi cant differences in the levels of auxin and several auxin-related genes, including AUX1, ARF, SAUR, and AUX/IAA, between the dormant stage (DS) and the owering stage (FS). Furthermore, it was reported that the IAA-inducible protein SAUR acts to inhibit the activity of the PP2C-type protein phosphatase, thereby in uencing plant growth and development in Rosa rugosa [25]. Their study revealed the upregulated expression of RrSAUR and the down-regulated expression of RrPP2C from dormant to initially differentiated oral bud, suggesting a potential negative regulation of RrPP2C by RrSAUR during the owering transition process.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of apical meristem enriched bud samples for size dependent flowering commitment in Crocus sativus reveal role of sugar and auxin signalling

Chaudhary,

Singh

2023

Preprint

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Background Cultivation of Crocus sativus (saffron) face challenges due to inconsistent flowering patterns and variations in yield. Flowering take place in a graded way with smaller corms being unable to produce flowers. Enhancing the productivity requires a comprehensive understanding of the underlying genetic mechanisms that govern this size based flowering initiation and commitment. Therefore, samples enriched with non-flowering and flowering apical buds from small (> 6g) and large (< 15g) corms were sequenced. Methods and Results Apical bud enriched samples from small and large corms were collected immediately after break of dormancy in month of July and performed RNA-sequencing on Illumina platform. De-novo transcriptome assembly and analysis using flowering committed buds from large corms at post-dormancy and their comparison with vegetative shoot primordia from small corms pointed out major role of Auxin and ABA hormonal regulation. Many genes with known dual response in flowering development and circadian rhythm like Flowering locus T and Cryptochrome 1 along with a transcript showing homology with small auxin upregulated RNA (SAUR) exhibited induced expression in flowering buds. Thorough prediction of Crocus sativus non-coding RNA repertoire has been carried out for the first time. Enolase was found to be acting as a major hub with protein-protein interaction analysis using Arabidopsis counterparts. Conclusion Transcripts belong to key pathways including phenylpropanoid biosynthesis, hormone signaling including and carbon metabolism were found significantly modulated. KEGG assessment and protein-protein interaction analysis conform the expression data. Findings unravel the genetic determinants driving the size-based flowering in Crocus sativus.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of apical meristem enriched bud samples for size dependent flowering commitment in Crocus sativus reveal role of sugar and auxin signalling

Chaudhary,

Singh

2023

Preprint

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“…Such alterations in secondary metabolites may help to regulate the salt tolerance of rose. Research on roses has focused mainly on flower quality, petal development, and flower bloom [ 5–7 ], and there are limited data available regarding signaling pathways linking plant development and secondary metabolites associated with salt stress.…”

Section: Introductionmentioning

confidence: 99%

Multi-omics analysis reveals key regulatory defense pathways and genes involved in salt tolerance of rose plants

Ren,

Yang,

Jing

et al. 2024

Horticulture Research

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Salinity stress causes serious damage to crops worldwide, limiting plant production. However, the metabolic and molecular mechanisms underlying the response to salt stress in rose (Rosa spp.) remain poorly studied. We therefore performed a multi-omics investigation of Rosa hybrida cv. Jardin de Granville (JDG) and Rosa damascena Mill. (DMS) under salt stress to determine the mechanisms underlying rose adaptability to salinity stress. Salt treatment of both JDG and DMS led to the buildup of reactive oxygen species (H2O2). Palisade tissue was more severely damaged in DMS than in JDG, while the relative electrolyte permeability was lower and the soluble protein content was higher in JDG than in DMS. Metabolome profiling revealed significant alterations in phenolic acid, lipids, and flavonoid metabolite levels in JDG and DMS under salt stress. Proteome analysis identified enrichment of flavone and flavonol pathways in JDG under salt stress. RNA sequencing showed that salt stress influenced primary metabolism in DMS, whereas it substantially affected secondary metabolism in JDG. Integrating these datasets revealed that the phenylpropane pathway, especially the flavonoid pathway, is strongly enhanced in rose under salt stress. Consistent with this, weighted gene coexpression network analysis (WGCNA) identified the key regulatory gene chalcone synthase 1 (CHS1), which is important in the phenylpropane pathway. Moreover, luciferase assays indicated that the bHLH74 transcription factor binds to the CHS1 promoter to block its transcription. These results clarify the role of the phenylpropane pathway, especially flavonoid and flavonol metabolism, in the response to salt stress in rose.

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Physiological and transcriptome analyses to infer regulatory networks in flowering transition of<i> Rosa rugosa</i>

Cited by 2 publications

References 53 publications

Transcriptome analysis of apical meristem enriched bud samples for size dependent flowering commitment in Crocus sativus reveal role of sugar and auxin signalling

Transcriptome analysis of apical meristem enriched bud samples for size dependent flowering commitment in Crocus sativus reveal role of sugar and auxin signalling

Multi-omics analysis reveals key regulatory defense pathways and genes involved in salt tolerance of rose plants

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