Physiological and Transcriptome Analyses of Transgenic FGF21 Immature Rice Seeds

Feng, Mingfang; Cai, Hua; Zhang, L. G.; Wu, Xinhui; Yu, Dahai; Huang, Xin; Li, B. W.; Wang, A. X.; Sun, Jian

doi:10.1134/s1021443720020041

Cited by 4 publications

(2 citation statements)

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“…Yeast (Pichia pastoris) was the rst organism for expression of rhFGF21 30 , and now several platforms have been reported to be capable of expressing rhFGF21, for instance, E. coli. 27 , Bacillus subtilis 29 and plants 33,34,38,39 . Plant expression platform is considered to be promising due to the lower cost, higher output, and higher biosecurity conserning 2,4,11,13,14 .…”

Section: Discussionmentioning

confidence: 99%

“…The expression of hFGF21 in microorganisms, although relatively high expression levels were obtained, the instability, solubility, and low bioactivity of the product 32 are still the issues awaited to be settled; moreover, the downstream puri cation processing and production cost are also issues needed to be concerned. Human FGF21 was also attempted to expressed in plants, including tobacco (Nicotiana benthamiana) 33,34 , tomato 35 , Arabidopsis 36 , carrot 37 and rice 38,39 . However, these expressions in plants, either stable or transient, are of low expression levels.…”

Section: Introductionmentioning

confidence: 99%

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Hyper Expression of Active, GFP-fused HFGF21 in Tobacco Chloroplasts

Wang

Fan

et al. 2022

Preprint

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Human fibroblast growth factor 21 (hFGF21) is a promising candidate for metabolic diseases. In this study, a tobacco chloroplast transformation vector, pWYP21406, was constructed. In the vector, the codon optimized encoding gene hFGF21 fused with GFP at its 5’ terminal was driven by the promoter of plastid rRNA operon (Prrn) and terminated by the terminator of plastid rps16 gene (Trps16). Spectinomycin resistant gene (aadA) as the selectable marker was placed in the same cistron between hFGF21 and the terminator Trps16. Transplastomic plants were generated by biolistic bombardment method and proven to be homoplastic by Southern Blotting analyses. The expression of GFP was detected under UV light and laser confocal microscope. The expression of GFP-hFGF21 was confirmed by immunoblotting and quantified by ELISA. The accumulation of was GFP-hFGF21confirmed to be 12.44 ± 0.45% of total soluble protein (i.e., 1.9232 ± 0.0673 g kg− 1 of fresh weight). The GFP-hFGF21 was confirmed to be capable of promoting the proliferation of hepatoma cell line HepG2, inducing the expression of glucose transporter 1 in hepatoma HepG2 cells and improve the glucose uptakes. The above results suggested that chloroplast expression is a promising approach for bioactive recombinant hFGF21 production.

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