Physiological and Pathological Mitochondrial Clearance Is Related to Pectoralis Major Muscle Pathogenesis in Broilers With Wooden Breast Syndrome

Hosotani, Marina; Kawasaki, Takeshi; Hasegawa, Yasuhiro; Wakasa, Yui; Hoshino, Maki; Takahashi, Naoki; Takaya, Tomohide; Iwasaki, Tomohito; Watanabe, Takafumi

doi:10.3389/fphys.2020.00579

Cited by 26 publications

(27 citation statements)

References 42 publications

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“…Histological results of the present work are similar to those presented by [54] who raised the hypothesis that the damage to mitochondria mediated by hypoxia serves as the first step in Wooden Breast pathogenesis, followed by muscle pathology such as hypertrophy and fibrosis. Praud et al [55] worked with molecular phenotyping of white striping and wooden breast myopathies in chicken and propose the use of histological and molecular tools allowing for precise quantification of the different lesions present in muscles affected by white striping or wooden breast.…”

Section: Discussionsupporting

confidence: 89%

“…The same authors say that molecular phenotyping will allow progress in understanding the etiology of these defects but also by refining the diagnosis of injuries accelerate the development of non-invasive prediction tools at the service of breeders and producers. The present work is an additional contribution in line with recent reports [54,55].…”

Section: Discussionsupporting

confidence: 89%

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Physical, Chemical and Histological Characterization of Pectoralis major Muscle of Broilers Affected by Wooden Breast Myopathy

Oliveira

Mello

Ferrari

et al. 2021

Animals

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This study aimed to characterize the effects of wooden breast myopathy (WBM) on quality of broiler chicken breast meat. Normal samples (absence of myopathy), moderate-degree samples (hardness only in one area of the breast fillet) and severe-degree samples (hardness throughout the breast fillet) were classified. In macroscopic analysis, the pectoral muscle affected by the WBM showed, in general, pale color with stiff, irregular and reddish regions (suffusions and petechiae), with the presence of white striations. In microscopic analysis, the myopathy was characterized by loss of the polygonal aspect of the muscle fibers. Samples with moderate degree of the myopathy showed greater (p = 0.0266) water retention capacity. There was an increase (p = 0.004) in total collagen concentration in samples from the severe-degree group 0.29% in normal samples to 0.43 and 0.48% in samples from moderate- and severe-degree groups, respectively. Samples of chicken breast affected by the severe-degree WBM showed lower (p < 0.0001) myofibrillar fragmentation index (64.51) and lower (p = 0.0002) fat concentration (2.17%) than normal chicken samples (80.45 and 3.79%, respectively). Samples affected by WBM are larger and heavier and present poorer physical quality when compared to normal chicken meat. Histologically it is possible to observe loss of the polygonal aspect of muscle fibers.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 89%

Physical, Chemical and Histological Characterization of Pectoralis major Muscle of Broilers Affected by Wooden Breast Myopathy

Oliveira

Mello

Ferrari

et al. 2021

Animals

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“…Thus, inhibition of this complex could have a dramatic influence on the energy production in WB tissues. The potential for diminished mitochondrial function in WB myopathy is supported by a recent report showing increased mitochondrial damage and mitophagy in histological analysis of WB myopathy [ 45 ]. Several studies provide evidence of enhanced mitochondrial function and complex activities in the high FE PedM phenotype (e.g., see review by [ 46 ]) that may be attributed to increased activity of PGC1α in the high FE phenotype shown in Figure 10 B.…”

Section: Resultsmentioning

confidence: 92%

Upstream Regulator Analysis of Wooden Breast Myopathy Proteomics in Commercial Broilers and Comparison to Feed Efficiency Proteomics in Pedigree Male Broilers

Bottje

Lassiter

Kuttappan

et al. 2021

Foods

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In an effort to understand the apparent trade-off between the continual push for growth performance and the recent emergence of muscle pathologies, shotgun proteomics was conducted on breast muscle obtained at ~8 weeks from commercial broilers with wooden breast (WB) myopathy and compared with that in pedigree male (PedM) broilers exhibiting high feed efficiency (FE). Comparison of the two proteomic datasets was facilitated using the overlay function of Ingenuity Pathway Analysis (IPA) (Qiagen, CA, USA). We focused on upstream regulator analysis and disease-function analysis that provides predictions of activation or inhibition of molecules based on (a) expression of downstream target molecules, (b) the IPA scientific citation database. Angiopoeitin 2 (ANGPT2) exhibited the highest predicted activation Z-score of all molecules in the WB dataset, suggesting that the proteomic landscape of WB myopathy would promote vascularization. Overlaying the FE proteomics data on the WB ANGPT2 upstream regulator network presented no commonality of protein expression and no prediction of ANGPT2 activation. Peroxisome proliferator coactivator 1 alpha (PGC1α) was predicted to be inhibited, suggesting that mitochondrial biogenesis was suppressed in WB. PGC1α was predicted to be activated in high FE pedigree male broilers. Whereas RICTOR (rapamycin independent companion of mammalian target of rapamycin) was predicted to be inhibited in both WB and FE datasets, the predictions were based on different downstream molecules. Other transcription factors predicted to be activated in WB muscle included epidermal growth factor (EGFR), X box binding protein (XBP1), transforming growth factor beta 1 (TGFB1) and nuclear factor (erythroid-derived 2)-like 2 (NFE2L2). Inhibitions of aryl hydrocarbon receptor (AHR), AHR nuclear translocator (ARNT) and estrogen related receptor gamma (ESRRG) were also predicted in the WB muscle. These findings indicate that there are considerable differences in upstream regulators based on downstream protein expression observed in WB myopathy and in high FE PedM broilers that may provide additional insight into the etiology of WB myopathy.

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“…It has not been strictly compared yet whether cellular properties of chicken myoblasts are distinctive for muscle tissues. The broiler breast muscle, which is mostly composed of fast-type myofibers frequently shows acute and chronic inflammatory and myopathic changes (Kuttappan et al, 2013;Hosotani et al, 2020). Intriguingly, ERVK transcription is known to be activated by pathogenic transcription factors such as NF-κB and interferon regulatory factors (Manghera and Douville, 2013).…”

Section: Discussionmentioning

confidence: 99%

Transcription of Endogenous Retrovirus Group K Members and Their Neighboring Genes in Chicken Skeletal Muscle Myoblasts

et al. 2021

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Skeletal muscle myoblasts are myogenic precursor cells that generate myofibers during muscle development and growth. We recently reported that broiler myoblasts, compared to layer myoblasts, proliferate and differentiate more actively and promptly into myocytes, which corresponds well with the muscle phenotype of broilers. Furthermore, RNA sequencing (RNA-seq) revealed that numerous genes are differentially expressed between layer and broiler myoblasts during myogenic differentiation. Based on the RNA-seq data, we herein report that chicken myoblasts transcribe endogenous retrovirus group K member (ERVK) genes. In total, 16 ERVKs were highly expressed in layer myoblasts and two (termed BrK1 and BrK2) were significantly induced in broiler myoblasts. These transcribed ERVKs had a total of 182 neighboring genes within ±100 kb on the chromosomes, of which 40% were concentrated within ±10 kb of the ERVKs. We further investigated whether the transcription of ERVKs affects the expression of their neighboring genes. BrK1 had two neighboring genes; LOC107052719 was overlapping with BrK1 and downregulated in the broiler myoblasts, and FAM19A2 was upregulated in the broiler myoblasts as well as BrK1. BrK2 had 14 neighboring genes, and only one gene, LOC772243 , was differentially expressed between layer and broiler myoblasts. LOC772243 was overlapping with BrK2 and suppressed in the broiler myoblasts. These data indicate that the transcription of ERVKs may impact the expression of their neighboring genes in chicken myoblasts.

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Physiological and Pathological Mitochondrial Clearance Is Related to Pectoralis Major Muscle Pathogenesis in Broilers With Wooden Breast Syndrome

Cited by 26 publications

References 42 publications

Physical, Chemical and Histological Characterization of Pectoralis major Muscle of Broilers Affected by Wooden Breast Myopathy

Physical, Chemical and Histological Characterization of Pectoralis major Muscle of Broilers Affected by Wooden Breast Myopathy

Upstream Regulator Analysis of Wooden Breast Myopathy Proteomics in Commercial Broilers and Comparison to Feed Efficiency Proteomics in Pedigree Male Broilers

Transcription of Endogenous Retrovirus Group K Members and Their Neighboring Genes in Chicken Skeletal Muscle Myoblasts

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