Physiological and Pathological Functions of SLC26A6

Wang, Juan; Wang, Wenkang; Wang, Hui; Tuo, Biguang

doi:10.3389/fmed.2020.618256

Cited by 26 publications

(32 citation statements)

References 129 publications

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“…NKCC1 provides chlorine for apical secretion through CFTR by mediating chloride uptake at the basolateral pole of intestinal epithelial cells [ 96 ]. SLC26 is a conservative anion transporter family, and SLC26A6 has the most extensive exchange function in the family; it mediates the transport of Cl − /HCO 3 − and other anions, and plays an important role in ion homeostasis and acid-base balance [ 102 ]. Therefore, our results show that PP-MP exposure affects ion transport in the intestinal tract of mice.…”

Section: Discussionmentioning

confidence: 99%

Exposure to Polypropylene Microplastics via Oral Ingestion Induces Colonic Apoptosis and Intestinal Barrier Damage through Oxidative Stress and Inflammation in Mice

Jia

Han²,

Liu³

et al. 2023

Toxics

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Extensive environmental pollution by microplastics has increased the risk of human exposure to plastics. However, the biosafety of polypropylene microplastics (PP-MPs), especially of PP particles < 10 μm, in mammals has not been studied. Thus, here, we explored the mechanism of action and effect of exposure to small and large PP-MPs, via oral ingestion, on the mouse intestinal tract. Male C57BL/6 mice were administered PP suspensions (8 and 70 μm; 0.1, 1.0, and 10 mg/mL) for 28 days. PP-MP treatment resulted in inflammatory pathological damage, ultrastructural changes in intestinal epithelial cells, imbalance of the redox system, and inflammatory reactions in the colon. Additionally, we observed damage to the tight junctions of the colon and decreased intestinal mucus secretion and ion transporter expression. Further, the apoptotic rate of colonic cells significantly increased after PP-MP treatment. The expression of pro-inflammatory and pro-apoptosis proteins significantly increased in colon tissue, while the expression of anti-inflammatory and anti-apoptosis proteins significantly decreased. In summary, this study demonstrates that PP-MPs induce colonic apoptosis and intestinal barrier damage through oxidative stress and activation of the TLR4/NF-κB inflammatory signal pathway in mice, which provides new insights into the toxicity of MPs in mammals.

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Section: Discussionmentioning

confidence: 99%

Exposure to Polypropylene Microplastics via Oral Ingestion Induces Colonic Apoptosis and Intestinal Barrier Damage through Oxidative Stress and Inflammation in Mice

Jia

Han²,

Liu³

et al. 2023

Toxics

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“…Genes C3 and C5 , as components of complement system, were specifically found in pig ileum (C4). Anion transporter SLC26A6 , mediating chloride absorption and bicarbonate secretion in the small intestine (Seidler et al, 2008 ; Wang et al, 2020a ), was expressed in mouse and rat ileum (C5). The aspartic protease NAPSA was enriched in rat ileum, consistent with protein proteolysis in functional enrichment analysis (C6).…”

Section: Resultsmentioning

confidence: 99%

Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium

Wang

et al. 2022

Cell Regen

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Animal models are widely used for biomedical studies and drug evaluation. The small intestine plays key roles in nutrient absorption, hormone secretion, microbiota defense and drug absorption and metabolism. Although the intestinal structure of mammals is conserved, the differences on epithelial cell composition, functional assignments and drug absorption among mammals are largely unknown. Here, cross-species analysis of single-cell transcriptomic atlas of the ileum epithelium from mouse, rat, pig, macaque and human reveals the conserved and differential cell types and functions among species, identifies a new CA7+ cell type in pig, macaque and human ileum, uncovers the distinct expression pattern in enterocytes, enteroendocrine cells and Paneth cells, and defines the conserved and species-specific intestinal stem cell signature genes. The examination of drug absorption across species suggests that drug metabolism in mouse ileum is closer to human while drug transport in macaque ileum is more similar to human. Together, our data provide the comprehensive information about cell composition and functional assignments in five species, and offer the valuable guidance for animal model selection and drug testing.

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“…In EOs, strong Slc26a6 expression was found at both the mRNA and protein level, whereas Slc26a3 expression was weak and non-specific. The Slc26a6 transporter is primarily located on the apical membrane of secretory epithelial cells, in which it plays an essential role in HCO 3 − secretion (29). Because the esophageal epithelium is not a typical secretory epithelium, the presence of this transporter on EOs is unusual.…”

Section: Discussionmentioning

confidence: 99%

Mouse organoid culture is a suitable model to study esophageal ion transport mechanisms

Korsós

Bellák

Becskeházi

et al. 2021

American Journal of Physiology-Cell Physiology

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Altered esophageal ion transport mechanisms play a key role in inflammatory and cancerous diseases of the esophagus, but epithelial ion processes have been less studied in the esophagus because of the lack of a suitable experimental model. In this study, we generated 3D esophageal organoids (EOs) from two different mouse strains and characterized the ion transport processes of the EOs. EOs form a cell-filled structure with a diameter of 250-300 µm and generated from epithelial stem cells as shown by FACS analysis. Using conventional PCR and immunostaining, the presence of Slc26a6 Cl−/HCO3− anion exchanger (AE), Na+/H+ exchanger (NHE), Na+/HCO3- cotransporter (NBC), cystic fibrosis transmembrane conductance regulator (CFTR) and anoctamin 1 Cl− channels were detected in EOs. Microfluorimetric techniques revealed high NHE, AE, and NBC activities, whereas that of CFTR was relatively low. In addition, inhibition of CFTR led to functional interactions between the major acid-base transporters and CFTR. We conclude that EOs provide a relevant and suitable model system for studying the ion transport mechanisms of esophageal epithelial cells, and they can be also used as preclinical tools to assess the effectiveness of novel therapeutic compounds in esophageal diseases associated with altered ion transport processes.

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Physiological and Pathological Functions of SLC26A6

Cited by 26 publications

References 129 publications

Exposure to Polypropylene Microplastics via Oral Ingestion Induces Colonic Apoptosis and Intestinal Barrier Damage through Oxidative Stress and Inflammation in Mice

Exposure to Polypropylene Microplastics via Oral Ingestion Induces Colonic Apoptosis and Intestinal Barrier Damage through Oxidative Stress and Inflammation in Mice

Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium

Mouse organoid culture is a suitable model to study esophageal ion transport mechanisms

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