Physiological and multi-omics responses of Neoporphyra haitanensis to dehydration-rehydration cycles

Abstract

Background Seaweeds in the upper intertidal zone experience extreme desiccation during low tide, followed by rapid rehydration during high tide. Porphyra sensu lato are typical upper intertidal seaweeds. Therefore, it is valuable to investigate the adaptive mechanisms of seaweed in response to dehydration-rehydration stress. Results A reduction in photosynthetic capacity and cell shrinkage were observed when N. haitanensis was dehydrated, and such changes were ameliorated once rehydrated. And the rate and extent of rehydration were affected by the air flow speed, water content before rehydration, and storage temperature and time. Rapid dehydration at high air-flow speed and storage at − 20 °C with water content of 10% caused less damage to N. haitanensis and better-protected cell activity. Moreover, proteomic and metabolomic analyses revealed the abundance members of the differentially expressed proteins (DEPs) and differentially abundant metabolites (DAMs) mainly involved in antioxidant system and osmotic regulation. The ascorbic acid-glutathione coupled with polyamine antioxidant system was enhanced in the dehydration response of N. haitanensis. The increased soluble sugar content, the accumulated polyols, but hardly changed (iso)floridoside and insignificant amount of sucrose during dehydration indicated that polyols as energetically cheaper organic osmolytes might help resist desiccation. Interestingly, the recovery of DAMs and DEPs upon rehydration was fast. Conclusions Our research results revealed that rapid dehydration and storage at − 20 °C were beneficial for recovery of N. haitanensis. And the strategy to resist dehydration was strongly directed toward antioxidant activation and osmotic regulation. This work provided valuable insights into physiological changes and adaptative mechanism in desiccation, which can be applied for seaweed farming.

Altered rumen microbiome and correlations of the metabolome in heat-stressed dairy cows at different growth stages

“…Combined with the enrichment of genes (GHs) involved in carbohydrate decomposition in the rumen microbiota of dairy cows in the H-N group, we further demonstrated that heifers in the H-N group had high ability to degrade complex substrates. Ascorbic acid and aldose metabolism are indirectly associated with biosynthetic pathways with significant antioxidant potential ( 33 ). Significant enrichment in the H-N group indicated a high antioxidant capacity, while the oxidative phosphorylation pathway showed a significant enrichment of the oxidative phosphorylation pathway in the H-HS group.…”

Physiological properties of Neoporphyra haitanensis conchocelis in response to different temperature

“…Floridoside and isofloridoside increased under cold stress to enhance osmotic resistance in Neoporphyra haitanensis (Zhu et al, 2022). Myo‐inositol and sorbitol were higher in the 5% relative water content group than the hydration group (Wang et al, 2022).…”