Physiological and Morphological Characteristics of Acidophilic Bacteria Leptospirillum ferriphilum and Acidithiobacillus thiooxidans, Members of a Chemolithotrophic Microbial Consortium

Panyushkina, Anna; Цаплина, И. А.; Tf, Kondrat'eva; Белый, А. В.; Булаев, А. Г.

doi:10.1134/s0026261718030086

Cited by 13 publications

(11 citation statements)

References 28 publications

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“…Isolation of pure cultures was carried out in 250 mL Erlenmeyer flasks containing 100 mL of the medium and 10% (v/v) of the inoculum. The flasks were incubated on Unimax-1010 rotor shakers (Heidolph Instruments, Schwabach, Germany; 170 rpm) in Inkubator-1000 thermostats (Heidolph Instruments, Schwabach, Germany) at 40 • C. The DNA extraction and purification, PCR amplification of the 16S rRNA gene fragments, amplicon purification and subsequent sequencing, as well as phylogenetic identification of the pure microbial cultures were carried out as previously described [21].…”

Section: Isolation Of Pure Cultures and Taxonomic Researchmentioning

confidence: 99%

Distinct Roles of Acidophiles in Complete Oxidation of High-Sulfur Ferric Leach Product of Zinc Sulfide Concentrate

Muravyov¹,

Panyushkina²

2020

Microorganisms

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A two-step process, which involved ferric leaching with biologically generated solution and subsequent biooxidation with the microbial community, has been previously proposed for the processing of low-grade zinc sulfide concentrates. In this study, we carried out the process of complete biological oxidation of the product of ferric leaching of the zinc concentrate, which contained 9% of sphalerite, 5% of chalcopyrite, and 29.7% of elemental sulfur. After 21 days of biooxidation at 40 °C, sphalerite and chalcopyrite oxidation reached 99 and 69%, respectively, while the level of elemental sulfur oxidation was 97%. The biooxidation residue could be considered a waste product that is inert under aerobic conditions. The results of this study showed that zinc sulfide concentrate processing using a two-step treatment is efficient and promising. The microbial community, which developed during biooxidation, was dominated by Acidithiobacillus caldus, Leptospirillum ferriphilum, Ferroplasma acidiphilum, Sulfobacillus thermotolerans, S. thermosulfidooxidans, and Cuniculiplasma sp. At the same time, F. acidiphilum and A. caldus played crucial roles in the oxidation of sulfide minerals and elemental sulfur, respectively. The addition of L. ferriphilum to A. caldus during biooxidation of the ferric leach product proved to inhibit elemental sulfur oxidation.

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Section: Isolation Of Pure Cultures and Taxonomic Researchmentioning

confidence: 99%

Distinct Roles of Acidophiles in Complete Oxidation of High-Sulfur Ferric Leach Product of Zinc Sulfide Concentrate

Muravyov¹,

Panyushkina²

2020

Microorganisms

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“…In the planktonic sample, the most dominant species was Leptospirillum ferriphilum (61%) which only feeds on ferrous [60]. Cuniculiplasma divulgatum growing organoheterotrophically accounted for 20% [61].…”

Section: Attached Microbial Comminity On Pyrite Residualmentioning

confidence: 99%

“…The sulfur-oxidizing bacterium A. caldus became the most dominant species and the ferric-oxidizing bacterium L. ferriphilum decreased dramatically in sessile consortia under 750 and 850 mV, proving that the attached microbes enhance pyrite leaching by improving sulfur oxidation efficiency. In the planktonic sample, the most dominant species was Leptospirillum ferriphilum (61%) which only feeds on ferrous [60]. Cuniculiplasma divulgatum growing organoheterotrophically accounted for 20% [61].…”

Section: Attached Microbial Comminity On Pyrite Residualmentioning

confidence: 99%

Contributions of Microbial “Contact Leaching” to Pyrite Oxidation under Different Controlled Redox Potentials

et al. 2020

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The function of microbial contact leaching to pyrite oxidation was investigated by analyzing the differences of residue morphologies, leaching rates, surface products, and microbial consortia under different conditions in this study. This was achieved by novel equipment that can control the redox potential of the solution and isolate pyrite from microbial contact oxidation. The morphology of residues showed that the corrosions were a little bit severer in the presence of attached microbes under 750 mV and 850 mV (vs. SHE). At 650 mV, the oxidation of pyrite was undetectable even in the presence of attached microbes. The pyrite dissolution rate was higher with attached microbes than that without attached microbes at 750 mV and 850 mV. The elemental sulfur on the surface of pyrite residues with sessile microorganisms was much less than that without attached microbes at 750 mV and 850 mV, showing that sessile acidophiles may accelerate pyrite leaching by reducing the elemental sulfur inhibition. Many more sulfur-oxidizers were found in the sessile microbial consortium which also supported the idea. The results suggest that the microbial “contact leaching” to pyrite oxidation is limited and relies on the elimination of elemental sulfur passivation by attached sulfur-oxidizing microbes rather than the contact oxidation by EPS-Fe.

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“…spp., Leptospirillum ( L. ) spp., Sulfobacillus ( Sb. ) spp., Ferroplasma ( F. ) spp., and some other microorganisms [ 15 , 17 , 18 , 19 , 20 , 21 , 22 ]. The Olympiada biooxidation plant is located in the Krasnoyarsk Krai (Siberia, Russia) and has been operated by Polyus Krasnoyarsk JSC since 2001.…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Central Role of Sulfur-Oxidizing Acidiphilium multivorum LMS in Industrial Bioprocessing of Gold-Bearing Sulfide Concentrates

Panyushkina

Булаев

Белый³

2021

Microorganisms

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Acidiphilium multivorum LMS is an acidophile isolated from industrial bioreactors during the processing of the gold-bearing pyrite-arsenopyrite concentrate at 38–42 °C. Most strains of this species are obligate organoheterotrophs that do not use ferrous iron or reduced sulfur compounds as energy sources. However, the LMS strain was identified as one of the predominant sulfur oxidizers in acidophilic microbial consortia. In addition to efficient growth under strictly heterotrophic conditions, the LMS strain proved to be an active sulfur oxidizer both in the presence or absence of organic compounds. Interestingly, Ac. multivorum LMS was able to succeed more common sulfur oxidizers in microbial populations, which indicated a previously underestimated role of this bacterium in industrial bioleaching operations. In this study, the first draft genome of the sulfur-oxidizing Ac. multivorum was sequenced and annotated. Based on the functional genome characterization, sulfur metabolism pathways were reconstructed. The LMS strain possessed a complicated multi-enzyme system to oxidize elemental sulfur, thiosulfate, sulfide, and sulfite to sulfate as the final product. Altogether, the phenotypic description and genome analysis unraveled a crucial role of Ac. multivorum in some biomining processes and revealed unique strain-specific characteristics, including the ars genes conferring arsenic resistance, which are similar to those of phylogenetically distinct microorganisms.

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Physiological and Morphological Characteristics of Acidophilic Bacteria Leptospirillum ferriphilum and Acidithiobacillus thiooxidans, Members of a Chemolithotrophic Microbial Consortium

Cited by 13 publications

References 28 publications

Distinct Roles of Acidophiles in Complete Oxidation of High-Sulfur Ferric Leach Product of Zinc Sulfide Concentrate

Distinct Roles of Acidophiles in Complete Oxidation of High-Sulfur Ferric Leach Product of Zinc Sulfide Concentrate

Contributions of Microbial “Contact Leaching” to Pyrite Oxidation under Different Controlled Redox Potentials

Unraveling the Central Role of Sulfur-Oxidizing Acidiphilium multivorum LMS in Industrial Bioprocessing of Gold-Bearing Sulfide Concentrates

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