Physiological and Molecular Changes during Lily Underground Stem Axillary Bulbils Formation

Zhang, Yang; Yong, Yubing; Wang, Q.; Lu, Yingzhi

doi:10.1134/s1021443718030172

Cited by 7 publications

(6 citation statements)

References 12 publications

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“…Histological and transcriptomic analysis showed that starch synthesis and accumulation was vital to bulblet emergence and development in triploid Lilium lancifolium. [12,13] Zhang et al [14] reported that the starch content of interior scales were higher than that of exterior scales. Starches from different tissue parts differed in colors, sizes, structure, morphology, and texture characteristics, which could affect the functional properties and their suitability for different industrial applications.…”

Section: Introductionmentioning

confidence: 99%

Morphological, structural, and physicochemical properties of starch isolated from different lily cultivars grown in China

Wang

Qun

et al. 2019

International Journal of Food Properties

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Starches were isolated from bulbs of seven lily cultivars grown in China and their morphological, structural, and physicochemical properties were investigated. All the isolated starch samples showed triangular, elliptical, less scalloped, round, and irregular shaped granules. The starch of different lily cultivars exhibited B-type crystalline pattern. The median diameters (D50) were found to be in range from 17.79 μm to 45.06 μm and the amylose content in range from 23.63 to 30.38 g/100 g starch. The weight average molar mass (M w) of amylopectin ranged from 4.03 × 10 7 g/mol to 11.00 × 10 7 g/mol and root-mean-square rotation radius (R z) ranged from 203.90 nm to 264.20 nm. HPAEC-PAD chain distribution analysis revealed that the average chain length of amylopectin ranged from DP 23.19-25.29. Starch samples of different lily cultivars showed peak viscosity ranging from 1699.7 cP to 2963.0 cP, and pasting temperature varying from 63.83°C to 71.00°C. Furthermore, the transition temperatures of the starch samples isolated from different lily cultivars were found to be in range from 50.76°C to 57.59°C for onset temperature (T o), 54.57°C to 64.30°C for peak temperature (T p), and 68.07°C to 77.16°C for conclusion temperature (T c). The gelatinization temperature range (ΔT r) was found to be from 16.66°C to 21.65°C and enthalpy (ΔH) was in range from 11.46 J/g to 16.29 J/g. These results indicated that lily starch properties were significantly different depending on lily variety, which could help in understanding the convenience of these starches for the application in different food and non-food industries.

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Section: Introductionmentioning

confidence: 99%

Morphological, structural, and physicochemical properties of starch isolated from different lily cultivars grown in China

Wang

Qun

et al. 2019

International Journal of Food Properties

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“…Previous studies mainly focused on the formation of lily bulbs under in vitro conditions, and very few studies have been carried out on their natural proliferation, especially in the absence of a clear molecular regulatory mechanism [1]. LA hybrid lily 'Aladdin' has a very strong self-propagation ability under natural conditions, and can spontaneously form a large number of good quality underground-stem bulblets on underground stems, which is an excellent test material to study the mechanism of lily bulb formation under natural conditions [2,3].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Insights into Lily Stem Bulblet Formation: Hormonal Regulation, Sugar Metabolism, and Transcriptional Networks in LA Lily ‘Aladdin’

Zhang,

Lyu,

Lyu

2024

Horticulturae

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Bulblets, as the main reproductive organ of lilies, have a tremendous impact on the reproductive efficiency of lilies. Cytokinin is known to promote the formation of lily bulblets, but little is known about the mechanisms involved. In this study, a combination of full-length transcriptome and high-throughput RNA sequencing (RNA-Seq) was performed at the leaf axils of LA lily ‘Aladdin’ to characterize the transcriptional response to 6-BA treatment during the critical period of stem-to-bulblet transition. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that 6-BA treatment caused significant changes in starch and sucrose metabolism and plant hormone balance. In particular, the high expression of SUS1 and TPS6 in the 6-BA-treated group suggests that sucrose may act as a key signal to promote bulblet initiation. Furthermore, the induction of elevated expression of genes associated with cytokinin and auxin transport and signaling is crucial for initiating bulblet emergence and stimulating growth. WGCNA analysis revealed that hub TFs such as BLHs, ARFs, HD-ZIPs, AP2/ERFs, and SBPs were significantly overexpressed with genes involved in carbohydrate metabolism and phytohormone signaling, which warranted more in-depth functional studies. This study enriches the understanding of plant hormone-related genes, sugar metabolism-related genes and various transcription factors in the regulation of plant organ development, and lays the foundation for further studies on the molecular mechanisms of lily stem bulblet formation.

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“…The function of the class I KNOX subfamily may differ between monocotyledons and dicotyledons ( Figure 1c ). LtKNOX1 was evolutionarily closely related to KNOX1 of Lilium ‘Aladdin’ ( Lilium longiflorum × Lilium asiatic ) (LiKNOX1) 31 in these species ( Figure 2 ). The Accession numbers of the sequences used in the phylogenetic tree are listed in Supplemental Table S3.…”

Section: Resultsmentioning

confidence: 99%

Overexpression of LtKNOX1 from Lilium tsingtauense in Nicotiana benthamiana affects the development of leaf morphology

Zhou

Fan

Zhao

et al. 2022

Plant Signaling & Behavior

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Leaves are the main vegetative organs of the aboveground part of plants and play an important role in plant morphogenesis. KNOTTED-LIKE HOMEOBOX ( KNOX ) plays a crucial role in regulating leaf cell fate and maintaining leaf development. In this study, we analyzed LtKNOX1 from Lilium tsingtauense and illustrated its function in transgenic plants. Tissue-specific expression analysis indicated that LtKNOX1 was highly expressed in stems, young flower buds, and shoot apical meristems (SAMs). Ectopic overexpression of LtKNOX1 in Nicotiana benthamiana suggested that transformants with mild phenotypes were characterized by foliar wrinkles and mildly curled leaves; transformants with intermediate phenotypes showed severely crimped blades and narrow leaf angles, and the most severe phenotypes lacked normal SAMs and leaves. Moreover, the expression levels of genes involved in the regulation of KNOX in transgenic plants were detected, including ASYMMETRIC LEAVES1, PIN-FORMED 1, GA20-oxidase, CUP-SHAPED COTYLEDON 2, CLAVATA 1 and WUSCHEL ( WUS ), and the expression of other genes were down-regulated except WUS . This study contributes to our understanding of the LtKNOX1 function.

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Physiological and Molecular Changes during Lily Underground Stem Axillary Bulbils Formation

Cited by 7 publications

References 12 publications

Morphological, structural, and physicochemical properties of starch isolated from different lily cultivars grown in China

Morphological, structural, and physicochemical properties of starch isolated from different lily cultivars grown in China

Transcriptional Insights into Lily Stem Bulblet Formation: Hormonal Regulation, Sugar Metabolism, and Transcriptional Networks in LA Lily ‘Aladdin’

Overexpression of LtKNOX1 from Lilium tsingtauense in Nicotiana benthamiana affects the development of leaf morphology

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