Physiological and iTRAQ‐Based Quantitative Proteomics Analysis of Methyl Jasmonate–Induced Tolerance in Brassica napus Under Arsenic Stress

Abstract

Brassica napus plants exposed to 200 μM arsenic (As) exhibited high-level of stress condition, which led to inhibited growth, enhanced lipid peroxidation, and disrupted cellular ultrastructures. Exogenous application of methyl jasmonate (MeJA) alleviated the As-induced oxidative stress and improved the plant growth and photosynthesis. In this study, changes in the B. napus leaf proteome are investigated in order to identify molecular mechanisms involved in MeJA-induced As tolerance. The study identifies 177 proteins that are differentially expressed in cultivar ZS 758; while 200 differentially expressed proteins are accumulated in Zheda 622, when exposed to As alone and MeJA+As treatments, respectively. The main objective was to identify the MeJA-regulated protein under As stress. Consistent with this, iTRAQ detected 61 proteins which are significantly accumulated in ZS 758 leaves treated with MeJA under As stress. While in Zheda 622, iTRAQ detected 49 MeJA-induced proteins under As stress. These significantly expressed proteins are further divided into five groups on the base of their function, that is, stress and defense, photosynthesis, carbohydrates and energy production, protein metabolism, and secondary metabolites. Taken together, this study sheds light on the molecular mechanisms involved in MeJA-induced As tolerance in B. napus leaves and suggests a more active involvement of MeJA in plant physiological processes.

Plant response to jasmonates: current developments and their role in changing environment

“…Ahmad et al (2017) have also suggested that JA mitigates the deleterious effects of Cd stress in Vicia faba by preventing the accumulation of Cd, H 2 O 2 , and MDA and by increasing proline, glycine betaine, SOD, CAT, APX, and GR activities which reduced the oxidative stress. Recently, Farooq et al (2018) have also reported that exogenous application of MJ to Brassica napus plants alleviated the arsenic-induced oxidative stress and improved overall plant growth and photosynthesis.…”

iTRAQ-Based Quantitative Proteomic Analysis of the Arabidopsis Mutant opr3-1 in Response to Exogenous MeJA

“…It can be seen that sequence coverages of the identified proteins were mostly below 30%, and most of the identified proteins were in the mass range 20-30 KD and 30-40 KD (Figure 1b,c). metabolism, and secondary metabolites [44]. Alvarez et al investigated the changes in protein redox regulation in response to oxidative stress induced by MeJA in Arabidopsis shoots and roots using quantitative proteomics approach and confirmed cysteine residues of proteins were involved in redox regulation, which provided a deeper understanding of the jasmonate signaling and regulation network [45].…”

“…Guo et al used an iTRAQ-based quantitative proteomics approach to analyze broccoli sprouts treated with exogenous jasmonic acid and found that photosynthesis and protein synthesis were inhibited after JA treatment, which was responsible for the slower growth of broccoli, but carbon metabolism and amino acid metabolism-related proteins were up-regulated [43]. Farooq et al investigated MeJA-induced Arsenic tolerance in Brassica napus leaves using iTRAQ and 110 differentially regulated proteins were identified-proteins that were involved in stress and defense, photosynthesis, carbohydrates and energy production, protein metabolism, and secondary metabolites [44]. Alvarez et al investigated the changes in protein redox regulation in response to oxidative stress induced by MeJA in Arabidopsis shoots and roots using quantitative proteomics approach and confirmed cysteine residues of proteins were involved in redox regulation, which provided a deeper understanding of the jasmonate signaling and regulation network [45].…”

Large Scale Proteomic Data and Network-Based Systems Biology Approaches to Explore the Plant World

“…In addition, only cysteine-containing peptides are analyzed, impairing the quantification of those that do not contain this aminoacid. Many more studies to quantify plant proteins have adopted isobaric tags for relative and absolute quantitation (iTRAQ) [ 108 , 109 , 110 ] and tandem mass tags (TMT) [ 111 ]. In comparison to ICAT, or ICPL [ 112 ], TMT and iTRAQ labeling takes place at the peptide level by means of amine specific reagents which assure a wider peptide labeling without loss of PTM information.…”