Physiological and genomic characterization of an exopolysaccharide-producing Weissella cibaria CH2 from cheese of the western Himalayas

Kumari, Meena; Kumar, Rakshak; Singh, Dharam; Bhatt, Shriya; Gupta, Mahesh

doi:10.1016/j.fbio.2020.100570

Cited by 16 publications

(19 citation statements)

References 58 publications

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“…In this study, no virulence and antibiotic resistance genes were found in the genome of W. cibaria NM1 through the RGI analysis. A similar finding on safety assessment was also reported for W. cibaria CH2 through genome screening of antibiotic resistance (Kumari et al, 2020).…”

Section: Probiotic Assessment Through Genome Screeningsupporting

confidence: 85%

“…Screening of probiotic-associated genes of W. cibaria NM1 was done through BLASTP search (Camacho et al, 2009) based on the probiotic-associated genes reported in the work of Kumari et al (2020). The protein sequences of W. cibaria NM1 were searching for antimicrobial resistance genes using ResFinder3.0 server (https://cge.cbs.dtu.dk/services/ResFinder/) (Zankari et al, 2012) and through Resistome data (RGI) from the Comprehensive Antibiotic Resistance Database (CARD; https://card.mcmaster.ca) (Alcock et al, 2020).…”

Section: In Silico Screening Of Probiotic-associated Genesmentioning

confidence: 99%

“…The genome screening of probiotic-associated genes of W. cibaria NM1 was done using BLASTP search and based on the closely related W. cibaria CH2 reported by Kumari et al (2020). Table 3 shows the list of probioticassociated genes present in the W. cibaria NM1 genome.…”

Section: Genome Screening Of Probiotic-associated Genesmentioning

confidence: 99%

“…Weissella cibaria has been studied for its probiotic potentials (Lee et al, 2012;Kang et al, 2019;Kumari et al, 2020). Probiotic is defined by Food and Agriculture Organization as a live microorganism which, when administered in sufficient amounts, convene a health value to the host (FAO/ WHO, 2001).…”

Section: Probiotic Assessment Through Genome Screeningmentioning

confidence: 99%

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In silico prediction and in vitro analysis of bacteriocin and probiotic properties of Weissella cibaria NM1 isolated from Asian sea bass

Malek¹,

Lokman²,

Tan³

et al. 2021

MJM

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Aims: This study aims to predict the presence of bacteriocin-and probiotic-associated genes in the genome of Weissella cibaria NM1 isolated from Asian sea bass using a machine learning-based NeuBI prediction approach, followed by the investigation of the crude bacteriocin antimicrobial and probiotic properties via in vitro analysis. Methodology and results: This study utilized the machine learning-based NeuBI prediction approach with a homology search of highly conserved bacteriocin-associated genes present in the genome of W. cibaria NM1. This approach discovered a putative bacteriocin gene (WC_2064) and bacteriocin operon with complete immunity, transporter, regulator and modifier genes. Furthermore, the genome of W. cibaria NM1 was found to harboured specific probioticassociated genes that would contribute to acid and bile tolerance, adhesion on the host cell and exhibited cholesterolreducing ability. On top of that, the genome also shows the absence of virulence and antibiotic resistance genes, which signifies the safety of W. cibaria NM1 as a potential probiotic candidate. In vitro study has confirmed the antipseudomonal activity of crude bacteriocin NM1 with MIC of 62.5 mg/mL. Weissella cibaria NM1 can tolerate 0.3% (v/v) of bile salt condition and the transit through the simulated gastric (pH 3 and 4) and small intestinal (pH 8) tract. Conclusion, significance and impact of study: Current findings suggested in silico approach can speed up the search for putative bacteriocin and probiotic-associated genes from the genome of W. cibaria NM1. Nevertheless, further verification through experimental works will be deemed essential.

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Section: Probiotic Assessment Through Genome Screeningsupporting

confidence: 85%

Section: In Silico Screening Of Probiotic-associated Genesmentioning

confidence: 99%

Section: Genome Screening Of Probiotic-associated Genesmentioning

confidence: 99%

Section: Probiotic Assessment Through Genome Screeningmentioning

confidence: 99%

See 2 more Smart Citations

In silico prediction and in vitro analysis of bacteriocin and probiotic properties of Weissella cibaria NM1 isolated from Asian sea bass

Malek¹,

Lokman²,

Tan³

et al. 2021

MJM

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“…Several studies have investigated the molecular processes involved in polysaccharide synthesis by LAB [ 28 , 29 , 30 , 31 ], and some authors have explored the genome of W. cibaria strains to examine some metabolic traits, in particular EPS production [ 32 , 33 ]. Genomic analysis showed the presence of a single extracellular dextransucrase ( dsr ) enzyme encoded by the dsr gene involved in the dextran synthesis.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Dextran Produced by the Food-Related Strain Weissella cibaria C43-11 and of the Relevant Dextransucrase Gene

Bellis

Ferrara

Bavaro

et al. 2022

Foods

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A metabolic feature of lactic acid bacteria (LAB) is the production of exopolysaccharides (EPSs), which have technological and functional properties of interest to the food sector. The present study focused on the characterization of the Weissella cibaria strain C43-11, a high EPS producer in the presence of sucrose, in comparison with a low-producing strain (C2-32), and on possible genetic regulatory elements responsible for the modulation of dextransucrase (dsr) genes expression. NMR analysis of the polymeric material produced by the C43-11 strain indicated the presence of dextran consisting mainly of a linear scaffold formed by α-(1–6) glycosidic linkages and a smaller amounts of branches derived from α-(1–2), α-(1–3), and α-(1–4) linkages. Molecular analysis of the dsr genes and the putative transcriptional promoters of the two strains showed differences in their regulatory regions. Such variations may have a role in the modulation of dsr expression levels in the presence of sucrose. The strong upregulation of the dsr gene in the C43-11 strain resulted in a high accumulation of EPS. This is the first report showing differences in the regulatory elements of the dsr gene in W. cibaria and indicates a new perspective of investigation to identify the regulatory mechanism of EPS production.

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Exopolysaccharide producing microorganisms for functional food industry

Baruah

Rajshee

Halami

2022

Current Developments in Biotechnology and Bioengineering

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Physiological and genomic characterization of an exopolysaccharide-producing Weissella cibaria CH2 from cheese of the western Himalayas

Cited by 16 publications

References 58 publications

In silico prediction and in vitro analysis of bacteriocin and probiotic properties of Weissella cibaria NM1 isolated from Asian sea bass

In silico prediction and in vitro analysis of bacteriocin and probiotic properties of Weissella cibaria NM1 isolated from Asian sea bass

Characterization of Dextran Produced by the Food-Related Strain Weissella cibaria C43-11 and of the Relevant Dextransucrase Gene

Exopolysaccharide producing microorganisms for functional food industry

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