Physiological and Genetic Adaptability in the Fungi

Sussman, Alfred S.

doi:10.1080/00275514.1957.12024609

Cited by 4 publications

(1 citation statement)

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“…Most information on the cell walls of fungi has been obtained by application of individual techniques to particular problems [e.g., chemical analysis (2, 7), X-ray diffraction (14), electron microscopy (3)], but the present time may be appropriate for a combined approach. This is particularly true in view of the recently recognized complexity of fungal cell walls (1), their role as physiologically and biochemically active structures (17), and their influence upon the morphogenesis of cells (4,6). Accordingly, this paper reports an investigation of the structure of cell walls of Neurospora crassa by electron microscopy both before and after enzymatic digestion, confirmation of chitin as a cell wall constituent by X-ray diffraction, and the amino acid composition of the residual protein component of the walls.…”

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confidence: 99%

Structure and Composition of the Cell Wall of Neurospora crassa

Manocha

Colvin

1967

J Bacteriol

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The structure and composition of the cell walls of hyphae of Neurospora crassa were investigated by electron microscopy, chemical analysis, and X-ray diffraction both before and after progressive enzymatic degradation by snail gut enzymes, chitinase, and trypsin. The wall consists of two phases: randomly disposed skeletal microfibrils of chitin only and an amorphous matrix which contains both #-glucans and protein. The protein contains a high percentage of the amides of aspartic and glutamic acid but no hydroxy-proline or cysteine. A portion of this protein is a component of or is associated with a system of pores which is embedded in the matrix of the wall. These pores, 40 to 70 A in outside diameter, sometimes branch and seem to provide a three-dimensional network from one side of the wall to the other. They may be a general system of transport across the walls.

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confidence: 99%