Physiological and gene transcription assays to assess responses of mussels to environmental changes

Counihan, Katrina L.; Bowen, Lizabeth; Ballachey, Brenda E.; Coletti, Heather A.; Hollmén, Tuula E.; Pister, Benjamin; Wilson, Tammy L.

doi:10.7717/peerj.7800

Cited by 4 publications

(7 citation statements)

References 97 publications

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“…Although differences in Pacific razor clams were identified among locations, the variances of physiological and gene transcription values within populations were relatively low when compared to data on bay mussels (Mytilus trossulus) collected from the same general locations in the two parks (Counihan et al, 2019). We suspect this may be due in part to lower variability in physical attributes (such as temperature and wave action) of razor clam habitat.…”

Section: Nmds Resultsmentioning

confidence: 73%

“…This study was conducted concurrently with a similar study on mussels, recently reported by Counihan et al (2019). Study areas (Lake Clark National Park and Preserve, LACL and Katmai National Park and Preserve, KATM) were the same although the specific collection substrates within each park varied for mussels (protected mixed-sediment) vs. razor clams (exposed soft sediment).…”

Section: Methodsmentioning

confidence: 94%

“…Study areas (Lake Clark National Park and Preserve, LACL and Katmai National Park and Preserve, KATM) were the same although the specific collection substrates within each park varied for mussels (protected mixed-sediment) vs. razor clams (exposed soft sediment). As analytical methods for the two studies were identical, data were collected as previously described in Counihan et al (2019), but the specific genes targeted in the mRNA transcription varied for each species.…”

Section: Methodsmentioning

confidence: 99%

“…On average, the condition factor of Pacific razor clams from LACL was 4.7 times higher and shells 1.6 times thicker than Pacific razor clams from KATM. In a concurrent study, LACL mussels had higher condition factors and thicker shells than KATM mussels (Counihan et al, 2019). The condition factor of Pacific razor clams at all KATM sites, except Swikshak in 2015, was below the range observed in Pacific razor clams held in a controlled environment with sufficient nutrients (K. Counihan, 2014, unpublished data).…”

Section: Nutrientsmentioning

confidence: 97%

“…The seven invertebrate biomarker assays detailed below were selected to assess the physiological status of the razor clams as an indicator of nearshore ecosystem health (Table 1) and have been used previously in our lab (Counihan, 2018;Counihan et al, 2019). The methods are based on published research on Mytilus spp.…”

Section: Invertebrate Biomarkersmentioning

confidence: 99%

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Monitoring nearshore ecosystem health using Pacific razor clams (Siliqua patula) as an indicator species

Bowen

Counihan

Ballachey

et al. 2020

PeerJ

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An emerging approach to ecosystem monitoring involves the use of physiological biomarker analyses in combination with gene transcription assays. For the first time, we employed these tools to evaluate the Pacific razor clam (Siliqua patula), which is important both economically and ecologically, as a bioindicator species in the northeast Pacific. Our objectives were to (1) develop biomarker and gene transcription assays with which to monitor the health of the Pacific razor clam, (2) acquire baseline biomarker and gene transcription reference ranges for razor clams, (3) assess the relationship between physiological and gene transcription assays and (4) determine if site-level differences were present. Pacific razor clams were collected in July 2015 and 2016 at three sites within each of two national parks in southcentral Alaska. In addition to determining reference ranges, we found differences in biomarker assay and gene transcription results between parks and sites which indicate variation in both large-scale and local environmental conditions. Our intent is to employ these methods to evaluate Pacific razor clams as a bioindicator of nearshore ecosystem health. Links between the results of the biomarker and gene transcription assays were observed that support the applicability of both assays in ecosystem monitoring. However, we recognize the need for controlled studies to examine the range of responses in physiology and gene transcripts to different stressors.

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Section: Nmds Resultsmentioning

confidence: 73%

Section: Methodsmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Nutrientsmentioning

confidence: 97%

Section: Invertebrate Biomarkersmentioning

confidence: 99%

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Monitoring nearshore ecosystem health using Pacific razor clams (Siliqua patula) as an indicator species

Bowen

Counihan

Ballachey

et al. 2020

PeerJ

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Effects of pollution on marine organisms

Mearns

Morrison

Arthur³

et al. 2020

Water Environment Research

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This review covers selected 2019 articles on the biological effects of pollutants, including human physical disturbances, on marine and estuarine plants, animals, ecosystems, and habitats. The review, based largely on journal articles, covers field, and laboratory measurement activities (bioaccumulation of contaminants, field assessment surveys, toxicity testing, and biomarkers) as well as pollution issues of current interest including endocrine disrupters, emerging contaminants, wastewater discharges, marine debris, dredging, and disposal. Special emphasis is placed on effects of oil spills and marine debris due largely to the 2010 Deepwater Horizon oil blowout in the Gulf of Mexico and proliferation of data on the assimilation and effects of marine debris microparticulates. Several topical areas reviewed in the past (e.g., mass mortalities ocean acidification) were dropped this year. The focus of this review is on effects, not on pollutant sources, chemistry, fate, or transport. There is considerable overlap across subject areas (e.g., some bioaccumulation data may be appeared in other topical categories such as effects of wastewater discharges, or biomarker studies appearing in oil toxicity literature). Therefore, we strongly urge readers to use keyword searching of the text and references to locate related but distributed information. Although nearly 400 papers are cited, these now represent a fraction of the literature on these subjects. Use this review mainly as a starting point. And please consult the original papers before citing them.

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Size and site specific transcriptomic responses of blue mussel (Mytilus edulis) to acute hypoxia

Hall,

Méthé,

Stewart-Clark

et al. 2023

Marine Genomics

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Physiological and gene transcription assays to assess responses of mussels to environmental changes

Cited by 4 publications

References 97 publications

Monitoring nearshore ecosystem health using Pacific razor clams (Siliqua patula) as an indicator species

Monitoring nearshore ecosystem health using Pacific razor clams (Siliqua patula) as an indicator species

Effects of pollution on marine organisms

Size and site specific transcriptomic responses of blue mussel (Mytilus edulis) to acute hypoxia

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