Physiological and comparative proteomic analyses of saline-alkali NaHCO3-responses in leaves of halophyte Puccinellia tenuiflora

Yin, Zepeng; Zhang, Heng; Zhao, Qi; Yoo, Mi‐Jeong; Zhu, Ning; Yu, Jianlan; Yu, Juanjuan; Guo, Siyi; Miao, Yuchen; Chen, Sixue; Qin, Zhi; Dai, Shaojun

doi:10.1007/s11104-019-03955-9

Cited by 54 publications

(56 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, Actin7 isoform 1 (Actin7, spot 23)-which is thought to aid in the stability of organelle membranes in plant cells and to induce PCD with the stabilization of actin depolymerization [44,45]-was significantly up-regulated under the NaCl and H 2 S treatment in our study. Meanwhile, plants trigger various enzymatic and non-enzymatic antioxidants to detoxify ROS and prevent cellular damage [46]. In this study, compared with the NaCl treatment, the content of GSH was found to be induced by application of H 2 S (Figure 2).…”

Section: H 2 S Relieves Oxidative Stress Induced By Naclmentioning

confidence: 60%

Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata

Liu

Shen

Simón

et al. 2019

IJMS

View full text Add to dashboard Cite

As a dominant mangrove species, Kandelia obovata is distributed in an intertidal marsh with an active H2S release. Whether H2S participates in the salt tolerance of mangrove plants is still ambiguous, although increasing evidence has demonstrated that H2S functions in plant responses to multiple abiotic stresses. In this study, NaHS was used as an H2S donor to investigate the regulatory mechanism of H2S on the salt tolerance of K. obovata seedlings by using a combined physiological and proteomic analysis. The results showed that the reduction in photosynthesis (Pn) caused by 400 mM of NaCl was recovered by the addition of NaHS (200 μM). Furthermore, the application of H2S enhanced the quantum efficiency of photosystem II (PSII) and the membrane lipid stability, implying that H2S is beneficial to the survival of K. obovata seedlings under high salinity. We further identified 37 differentially expressed proteins by proteomic approaches under salinity and NaHS treatments. Among them, the proteins that are related to photosynthesis, primary metabolism, stress response and hormone biosynthesis were primarily enriched. The physiological and proteomic results highlighted that exogenous H2S up-regulated photosynthesis and energy metabolism to help K. obovata to cope with high salinity. Specifically, H2S increased photosynthetic electron transfer, chlorophyll biosynthesis and carbon fixation in K. obovata leaves under salt stress. Furthermore, the abundances of other proteins related to the metabolic pathway, such as antioxidation (ascorbic acid peroxidase (APX), copper/zinc superoxide dismutase (CSD2), and pancreatic and duodenal homeobox 1 (PDX1)), protein synthesis (heat-shock protein (HSP), chaperonin family protein (Cpn) 20), nitrogen metabolism (glutamine synthetase 1 and 2 (GS2), GS1:1), glycolysis (phosphoglycerate kinase (PGK) and triosephosphate isomerase (TPI)), and the ascorbate–glutathione (AsA–GSH) cycle were increased by H2S under high salinity. These findings provide new insights into the roles of H2S in the adaptations of the K. obovata mangrove plant to high salinity environments.

show abstract

Section: H 2 S Relieves Oxidative Stress Induced By Naclmentioning

confidence: 60%

Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata

Liu

Shen

Simón

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…This suggests that Ca 2+ /Na + ratio could play a major role in predicting the SA-tolerance of alfalfa growing under realistic SA field conditions as compared with SL when both are simultaneously evaluated. Calcium is crucial for maintaining the structural and functional integrity of plant membranes, in addition to its function in the regulation of ion transport, selectivity, ion homeostasis maintenance, signal transduction, and activation of cell wall enzymes and antioxidant enzyme activities [19,20,34,73]. According to Suarez and Grieve (1988) [74], the Ca status of the plant shoot is strongly influenced by the ionic composition of the external medium; hence, the Ca 2+ /Na + ratio may be predicted from the solution composition of the root media and shoot ion concentrations under salinity-stressed conditions.…”

Section: Identification Of the Best Markers For Predicting The Salinementioning

confidence: 99%

“…It is well known that Mg 2+ is essential for protein synthesis and chlorophyll structure, being an activator for many photosynthetic and respiratory enzymes [37]. More importantly, the accumulation of cations, including Mg 2+ , as important nutrients, might also be an important mechanism in the plant response to SA stress by maintaining ion homeostasis [38,76] and contributing to signaling and enzyme activity [34]. Consistently, we found that the SATC of shoot Mg 2+ /Na + was significantly and positively related to the D-value (r = 0.74, P < 0.01) (Table S5).…”

Section: Identification Of the Best Markers For Predicting The Salinementioning

confidence: 99%

“…Agronomic characters represent the combined genetic and environmental effects upon plant growth and yield, as they include the physiological mechanisms conferring salinity and alkalinity tolerance [27,34,40,78]. When using the two methods, the SATC of SDM was separately identified as an important predictor and was significantly related to the SL (Table S5).…”

Section: Identification Of the Best Markers For Predicting The Salinementioning

confidence: 99%

See 1 more Smart Citation

Ca2+/Na+ Ratio as a Critical Marker for Field Evaluation of Saline-Alkaline Tolerance in Alfalfa (Medicago sativa L.)

et al. 2020

View full text Add to dashboard Cite

Current indices of saline-alkaline (SA) tolerance are mainly based on the traditional growth and physiological indices for salinity tolerance and likely affect the accuracy of alfalfa tolerance predictions. We determined whether the inclusion of soil alkalinity-affected indices, particularly Ca2+, Mg2+, and their ratios to Na+ in plants, based on the traditional method could improve the prediction accuracy of SA tolerance in alfalfa, determine important indices for SA tolerance, and identify suitable alfalfa cultivars in alkaline salt-affected soils. Fifty alfalfa cultivars were evaluated for their SA tolerance under SA and non-SA field conditions. The SA-tolerance coefficient (SATC) for each investigated index of the alfalfa shoot was calculated as the ratio of SA to non-SA field conditions, and the contribution of SATC under different growth and physiological indices to SA tolerance was quantified based on the inclusion/exclusion of special alkalinity-affected indices. The traditional method, excluding the special alkalinity-affected indices, explained nearly all of the variation in alfalfa SA tolerance, and the most important predictor was the SATC of stem length. The new method, which included these special alkalinity-affected indices, had similar explanatory power but instead identified the SATC of shoot Ca2+/Na+ ratio, followed by that of stem length, as key markers for the field evaluation of SA tolerance. Ca2+, Mg2+, and their ratios to Na+ hold promise for enhancing the robustness of SA-tolerance predictions in alfalfa. These results encourage further investigation into the involvement of Ca2+ in such predictions in other plant species and soil types under more alkaline salt-affected conditions.

show abstract

“…1i) but not significantly altered inleaves [32]. However, MDA contents were increased inleaves under Na 2 CO 3 (38 mM and 95 mM for seven days) and NaHCO 3 (150 mM, 400 mM, and 800 mM for seven days) [33,36]. This indicates different tissues may respond differently to neutral salt stress and alkali stress.…”

Section: Diverse Osmotic Regulation Strategies In Salt-stressed Callusmentioning

confidence: 88%

NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

Zhang

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Salinity has obvious effects on plant growth and crop productivity. The salinity-responsive mechanisms have been well-studied in differentiated organs (e.g., leaves, roots and stems), but not in unorganized cells such as callus. High-throughput quantitative proteomics approaches have been used to investigate callus development, somatic embryogenesis, organogenesis, and stress response in numbers of plant species. However, they have not been applied to callus from monocotyledonous halophyte alkaligrass (Puccinellia tenuifora). Results: The alkaligrass callus growth, viability and membrane integrity were perturbed by 50 mM and 150 mM NaCl treatments. Callus cells accumulated the proline, soluble sugar and glycine betaine for the maintenance of osmotic homeostasis. Importantly, the activities of ROS scavenging enzymes (e.g., SOD, APX, POD, GPX, MDHAR and GR) and antioxidants (e.g., ASA, DHA and GSH) were induced by salinity. The abundance patterns of 55 salt-responsive proteins indicate that salt signal transduction, cytoskeleton, ROS scavenging, energy supply, gene expression, protein synthesis and processing, as well as other basic metabolic processes were altered in callus to cope with the stress. Conclusions: The undifferentiated callus exhibited unique salinity-responsive mechanisms for ROS scavenging and energy supply. Activation of the POD pathway and AsA-GSH cycle was universal in callus and differentiated organs, but salinity-induced SOD pathway and salinity-reduced CAT pathway in callus were different from those in leaves and roots. To cope with salinity, callus mainly relied on glycolysis, but not the TCA cycle, for energy supply. Keywords: Salinity response, ROS scavenging, Energy supply, Osmotic homeostasis, Callus, Halophyte alkaligrass, Proteomics

show abstract

Physiological and comparative proteomic analyses of saline-alkali NaHCO3-responses in leaves of halophyte Puccinellia tenuiflora

Cited by 54 publications

References 88 publications

Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata

Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata

Ca2+/Na+ Ratio as a Critical Marker for Field Evaluation of Saline-Alkaline Tolerance in Alfalfa (Medicago sativa L.)

NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

Contact Info

Product

Resources

About