Physiological and Biochemical Studies on Virulent and Avirulent Isolates of <i>Agrobacterium tumefaciens</i> in Egypt

Abo-El-Dahab, M. K.; El‐Goorani, M. A.; Elwakil, M. A.

doi:10.1111/j.1439-0434.1978.tb04190.x

Cited by 6 publications

(4 citation statements)

References 15 publications

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“…Clark [34] compared 31 virulent and avirulent strains of .4. tumefaciens, 1 strain of A. rhizogenes and 3 strains of A. radiobacter for oxidase, phenol-oxidase, catalase and fl-glycosidase activity, quinone reduction and H202 production: most of them, except two strains, oxidized catechol and o-, m-or p-aminophenol); strains capable of producing reducing compounds from lactose grew at higher quinone (oxidized phenol) concentrations than non-producing strains. Another study made with 16 cultures of A. tumefaciens isolated in Egypt, revealed that all isolates tested showed no polyphenol oxidase activity, when grown on Ltyrosine and dihydroxyphenylalanine (DOPA) as substrates [35]. So, there seems to be a considerable variation in phenol oxidase activity among the Agrobacterium strains tested.…”

Section: Polyphenoloxidase Activity Among Agrobacterium Strainsmentioning

confidence: 99%

“…It is known that auxin is an important factor during crown gall transformation: the conversion of competent host cells to rapidly dividing cells is considered to involve at least two steps, the second requiring auxin [56,57]. It has also been demonstrated that Agrobacterium strains can synthetize fl-indoleacetic acid [35,58,59] and actually produce this plant-growth regulator in the host plant [60]. So a high ratio of poly-and diphenols to monophenols could be an important factor in protecting the plant growth factor.…”

Section: Auxin Protection Certain Phenolics Showmentioning

confidence: 99%

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The susceptibility of plants to Agrobacterium: A discussion of the role of phenolic compounds

Cleene¹

1988

FEMS Microbiology Letters

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Section: Polyphenoloxidase Activity Among Agrobacterium Strainsmentioning

confidence: 99%

Section: Auxin Protection Certain Phenolics Showmentioning

confidence: 99%

The susceptibility of plants to Agrobacterium: A discussion of the role of phenolic compounds

Cleene¹

1988

FEMS Microbiology Letters

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“…All of these bacteria, however, were not inhibitory when autoclaved before mixing. (2) Cross inoculation V-R, V, Av-R, and Av were crossinoculated to rice seedlings grown in the same way as in (1). Inoculation was made at 2 days interval at the points 1cm apart.…”

Section: Inhibition Of Lesion Enlargementmentioning

confidence: 99%

Variation in the Virulence of Xanthomonas oryzae (3)

Fujii

1976

Jpn. J. Phytopathol.

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Avirulent mutants of Xanthomonas oryzae occur during the course of successive culture on artificial media, and the population of these mutants gradually increases. In this experiment, mutual interference in multiplication between avirulent mutant (Av) and virulent wild type (V) in liquid media or inoculated rice leaves was studied. For the purpose of these studies, streptomycin resistant mutant from Av (Av-R), and that from V (V-R) were used frequently.Between Av and Av-R or between V and V-R, no difference could be found in virulence, colony morphology, lysotype, and bacterial physiology, except streptomycin resistance. Multiplication of V-R in liquid medium was markedly depressed by adding nearly the equal amount of Av. When V-R was mixed with Av and inoculated to rice leaves, lesion enlargement was inhibited in proportion to the amount of Av added. Multiplication of V-R in rice leaves was also inhibited by the addition of Av. Multiplication of Av-R in liquid medium was influenced very little by adding V.When Av-R was inoculated singly, it multiplied during 10-15 days within 2-3cm distance around the inoculated points, though no visible lesion was formed on the leaf.By mix-inoculation with Av-R+V, bacterial population of Av-R in leaves was much increased, and at the same time, Av-R was distributed within wider area in leaves.

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“…Ten most virulent and important bacterial plant pathogens based on their pathogenicity level, economic impact, and molecular aspects were listed1. Most devastating bacterial plant pathogens species were isolated in Egypt which belong to Pseudomonas syringae pathovars 3,4,5,6 , Ralstonia solanacearum 7,8,9 , Agrobacterium tumefaciens 9,10,11,12 , Xanthomonas 3,13,14 , Erwinia amylovora 15,16,17,18,19,20 and Xylella fastidiosa 21. Bacterial soft rot soft rots commonly affect vegetables and fruits. It can occur on crops in the eld and on the market.…”

Section: Introductionmentioning

confidence: 99%

Nano-Metals Forming Bacteria in Egypt. II. Efficacy towards Biomolecules, Ultrastructure, Growth Parameters, and Eco-Friendly Therapeutic of Soft Rot/Blackleg Genera

Shoeib

Ashmawy

Kamal

et al. 2022

Preprint

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The nanoparticles (NPs) formed by Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculate were tested against soft rot/blackleg genera. The effects of NPs recorded on bacterial DNA, proteins, and carbohydrates concentration of Pectobacterium carotovorum subsp. carotovorum, Enterobacter cloacae (soft rot), and Dickeya solani (soft rot/blackleg). Treated cells showed degradation in isolated DNA and decreased proteins and carbohydrates concentration compared with untreated cells. Using Scanning Electron Microscope (SEM), the treated cells showed collapsed and small pits in the cell wall. Using Transmission Electron Microscope (TEM), internal changes showed penetration of NPs inside the tested bacterial cells, the appearance of periplasmic space, formation of vacuoles, and condensation of cytoplasm. Disease severity ex vivo of potato tuber infected with tested genera demonstrated that NPs treatment didn't show any rotted tissue compared with untreated. The ability to uptake and accumulate FeNPs from the soil in potato (Solanum tuberosum) seedlings; Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) was used. ICP-OES recorded an increase in iron content of treated potato (Solanum tuberosum) seedlings with NPs, compared with untreated. FeNPs can be used to control soft rot/blackleg diseases; instead of copper pesticides. It could be a new, and alternative approach to traditional disease management methods and increase the plant's nutritional value.

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Physiological and Biochemical Studies on Virulent and Avirulent Isolates of Agrobacterium tumefaciens in Egypt

Cited by 6 publications

References 15 publications

The susceptibility of plants to Agrobacterium: A discussion of the role of phenolic compounds

The susceptibility of plants to Agrobacterium: A discussion of the role of phenolic compounds

Variation in the Virulence of Xanthomonas oryzae (3)

Nano-Metals Forming Bacteria in Egypt. II. Efficacy towards Biomolecules, Ultrastructure, Growth Parameters, and Eco-Friendly Therapeutic of Soft Rot/Blackleg Genera

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