Physiological and biochemical responses of Brassica napus L. cultivars exposed to Cd stress

Kovaliková, Zuzana; Komárková, Martina; Šimek, Jiri; Skarka, Adam; Tůma, Jiří

doi:10.17221/148/2022-pse

Cited by 3 publications

(3 citation statements)

References 40 publications

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“…The results showed that the activities of the SOD, POD, APX, and GSH of the cabbage seedlings were increased under Cd stress, while the exogenous application of Se further improved the activity of the cabbage seedling leaves (Figure 9), indicating that Se could significantly reduce the oxidative damage caused by Cd stress by enhancing the antioxidant response. Our results were consistent with those of Qi W et al who confirmed that Cd-stressed Brassica napus plants could enhance the activity of antioxidant enzymes, that the enzyme activity was further improved after the Se application, and that the antioxidant enzymes were closely related to Cd tolerance [40]. These results suggest that the exogenous Se may induce REDOX homeostasis by activating both enzyme-supported and non-enzyme-supported antioxidant systems, thereby protecting the cabbage seedlings from Cd stress.…”

Section: Discussionsupporting

confidence: 92%

“…In this study, we analyzed the physiological and biochemical parameters of cabbage seedlings regulated by the exogenous Se application under Cd stress. Under Cd stress, the cabbage plants showed obvious symptoms such as leaf rolling, yellowing, inhibition of root elongation, and a reduction of biomass (Figure 1), which was consistent with studies in Brassica napus, mustard, tomato, and cucumber [18,[38][39][40]. On the contrary, the exogenous application of Se could alleviate these phenomena, and the exogenous application of Se promoted the growth of the cabbage seedlings under Cd stress, which was reflected in the increase of plant height, stem diameter, and biomass (Figure 2A-G).…”

Section: Discussionsupporting

confidence: 89%

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Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings

Jia,

Zhan,

Wang

et al. 2023

Horticulturae

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In recent years, the levels of cadmium (Cd) in agricultural soils have been increasing. Cd is highly toxic and can enter the human body through the food chain, threatening human health, therefore, reducing the Cd content in vegetables and producing green and non-polluting food has become a common concern in society. However, the physiological properties of exogenous selenium in alleviating Cd stress in cabbage seedlings have not been thoroughly investigated. In this study, exogenous Se (10 μMol/L) was applied under Cd (25 μMol/L) stress and the physiological characteristics such as biomass, photosynthetic fluorescence parameters, Se and Cd contents, chloroplast ultrastructure, leaf membrane esterification, and antioxidant enzyme activities were determined. The results showed that the exogenous application of Se could effectively alleviate the decrease in growth, photosynthetic pigment, and the gas exchange characteristics of the cabbage seedlings under Cd stress, improve cabbage root vitality, reduce root leaf Cd content, and alleviate the Cd stress-induced damage. Ultrastructural observation showed that the Cd stress caused the disruption to the chloroplasts’ internal structure in the cabbage leaves, while an exogenous Se treatment alleviated the chloroplast damage to some extent, improved the stability of the inner capsule membrane, and alleviated the Cd stress-induced damage to the photosynthetic organs. Cd stress also caused oxidative damage and the excessive accumulation of reactive oxygen species (ROS) in the leaves of cabbage seedlings, as evidenced by the significant accumulation of superoxide anion (O2−), hydrogen peroxide (H2O2), malondialdehyde (MDA), and electrolyte leakage. On the other hand, after the exogenous Se treatment, the Cd stress-induced oxidative damage could be reduced by up-regulating the activities of the antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX). At the same time, Cd stress significantly increased glutathione (GSH) levels, and the exogenous Se treatment further increased the GSH levels, thereby increasing the tolerance of the cabbage to Cd stress. In conclusion, exogenous Se can further improve the Cd tolerance of the cabbage seedlings by protecting the photosynthetic system, eliminating excessive accumulation of reactive oxygen species under Cd stress, alleviating oxidative stress, and reducing Cd levels in plants, among other physiological properties.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings

Jia,

Zhan,

Wang

et al. 2023

Horticulturae

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“…In addition, Cd also signi cantly inhibited the enzymatic activity of SOD and CAT in sun owers, common beans, and peas [39]. The activities of GPX and APX in wheat and maize were increased under Cd stress, and the activity of POD in rape was minimized [40]. Based on the above analysis, a diagram of the pattern of sweet potato response to Cd stress is produced (Fig.…”

Section: Discussionmentioning

confidence: 98%

Effects of Cadmium Stress on the Growth and Physiological Characteristics of Sweet Potato in the Whole Growth Period

Ran,

Cao,

Xiao

et al. 2024

Preprint

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This study evaluated the responses of sweet potatoes to Cd stress through pot experiments, thus laying a theoretical foundation for their comprehensive utilization in Cd-polluted cultivated land. Four treatments were designed in the experiments, i.e., CK and three Cd stress treatments (T1, T2, and T3) with Cd concentrations of 3, 30, and 150 mg/kg, respectively. Through analyzing the individual plant growth, photosynthesis, antioxidant capacity, carbohydrate Cd accumulation distribution, and other indicators of sweet potato at different growth periods, the characteristics of sweet potato carbon metabolism in response to Cd stress during the whole growth period were explored. The results showed that T2 and T3 treatments inhibited the vine length, leaf area, stem diameter, and tuberous root growth of sweet potato, while T3 treatment significantly increased the number of sweet potato branches. Under Cd stress, the synthesis of chlorophyll in sweet potato was significantly inhibited, while the activity of the Rubisco enzyme was significantly reduced. As the Cd concentration increased, the function of PS II was also affected. The soluble sugar content showed no significant change in low Cd concentration treatments but decreased significantly under high Cd concentrations; the tuberous root starch content decreased significantly with the increase in Cd concentration. During the whole growth period, the activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) increased significantly in T2 and T3 treatments, while the SOD activity in T1 treatment was significantly lower than that of CK. With the increase in Cd concentration, the Cd contents of various sweet potato organs exhibited increasing trends. The absorbing roots had the highest bioconcentration factor, the tuberous roots had a lower bioconcentration factor and Cd accumulation, and the transfer factor from stem to petiole was the highest. These results showed that sweet potatoes had a high Cd tolerance and a certain restoration potential for Cd-contaminated cultivated land.

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Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (<em>Brassica oleracea</em> L. Var. Capitata) Seedlings

Jia,

Zhan,

Wang

et al. 2023

Preprint

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Cabbage (Brassica oleracea L. var. capitata) plays a very important role in the annual national vegetable supply and export trade. In recent years, the cadmium content of agricultural soils has increased. As a result, cabbage yields and quality have declined significantly. Studies have shown that selenium can counteract the harmful effects of cadmium on plants and can alleviate the stress caused by cadmium during the growth of plants. However, the mechanism of exogenous selenium application in the alleviation of cadmium stress in cabbage seedlings has not been thoroughly investigated. In this study, exogenous selenium (10μMol/L) was applied under cadmium (25μMol/L) stress and the physiological mechanisms such as biomass, photosynthetic pigment, leaf stomata parameters, selenium and cadmium content, chloroplast ultrastructure, active oxygen accumulation, leaf membrane esterification and antioxidant enzyme activity were determined. The protective mechanism of exogenous selenium on cabbage seedlings under cadmium stress was investigated. The re-sults showed that exogenous application of selenium could effectively alleviate the decrease in growth, photosynthetic pigment and gas exchange characteristics of cabbage seedlings under cadmium stress, im-prove cabbage root vitality, reduce root leaf cadmium content and alleviate cadmium stress-induced damage. Ultrastructural observation showed that cadmium stress caused the disruption of the internal structure of chloroplasts of cabbage leaves, while exogenous selenium treatment alleviated the chloroplast damage to some extent, improved the stability of the inner capsule membrane and alleviated the cadmium stress-induced damage to photosynthetic organs. Cadmium stress also caused oxidative damage and ex-cessive accumulation of ROS in the leaves of cabbage seedlings as evidenced by significant accumulation of O2-, H2O2, MDA and electrolyte leakage. On the other hand, after exogenous selenium treatment, cadmium stress- induced oxidative damage could be reduced by up-regulating the activities of antioxidant enzymes SOD, POD and APX. At the same time, cadmium stress significantly increased GSH levels, and exogenous selenium treatment further increased GSH levels, thereby increasing the tolerance of cabbage to cadmium stress. In conclusion, by protecting the photosynthetic system, eliminating excessive accumulation of reactive oxygen species in cadmium-stressed cabbage seedlings, alleviating oxidative stress and reducing cadmium levels in the plant, exogenous selenium can further improve cadmium tolerance in cabbage seedlings.

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Physiological and biochemical responses of Brassica napus L. cultivars exposed to Cd stress

Cited by 3 publications

References 40 publications

Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings

Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings

Effects of Cadmium Stress on the Growth and Physiological Characteristics of Sweet Potato in the Whole Growth Period

Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (<em>Brassica oleracea</em> L. Var. Capitata) Seedlings

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