Physiological and Biochemical Regulation Mechanism of Exogenous Hydrogen Peroxide in Alleviating NaCl Stress Toxicity in Tartary Buckwheat (Fagopyrum tataricum (L.) Gaertn)

Abstract

We aimed to elucidate the physiological and biochemical mechanism by which exogenous hydrogen peroxide (H2O2) alleviates salt stress toxicity in Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn). Tartary buckwheat “Chuanqiao-2” under 150 mmol·L−1 salt (NaCl) stress was treated with 5 or 10 mmol·L−1 H2O2, and seedling growth, physiology and biochemistry, and related gene expression were studied. Treatment with 5 mmol·L−1 H2O2 significantly increased plant height (PH), fresh and dry weights of shoots (SFWs/SDWs) and roots (RFWs/RDWs), leaf length (LL) and area (LA), and relative water content (LRWC); increased chlorophyll a (Chl a) and b (Chl b) contents; improved fluorescence parameters; enhanced antioxidant enzyme activity and content; and reduced malondialdehyde (MDA) content. Expressions of all stress-related and enzyme-related genes were up-regulated. The F3′H gene (flavonoid synthesis pathway) exhibited similar up-regulation under 10 mmol·L−1 H2O2 treatment. Correlation and principal component analyses showed that 5 mmol·L−1 H2O2 could significantly alleviate the toxic effect of salt stress on Tartary buckwheat. Our results show that exogenous 5 mmol·L−1 H2O2 can alleviate the inhibitory or toxic effects of 150 mmol·L−1 NaCl stress on Tartary buckwheat by promoting growth, enhancing photosynthesis, improving enzymatic reactions, reducing membrane lipid peroxidation, and inducing the expression of related genes.

Enhanced Antioxidant Activity and Secondary Metabolite Production in Tartary Buckwheat under Polyethylene Glycol (PEG)-Induced Drought Stress during Germination

“…The distinct clustering observed among all traits, particularly the identification of the XiNong 9943 genotype as exhibiting the highest drought tolerance. This result is consistent with prior research indicating the importance of RWC regulation, osmotic adjustment, and antioxidant defense mechanisms in conferring stress resilience [17,[65][66][67]. Overall, the integration of these analytical approaches enhances our understanding of genotypic responses to PEG-induced drought stress, elucidating key physiological and biochemical mechanisms underlying drought tolerance in Tartary buckwheat genotypes.…”

Effect of salinity on growth and biochemical responses of brinjal varieties: implications for salt tolerance and antioxidant mechanisms

“…Ion toxicity, osmotic imbalance, stunted plant growth, and deficiency in nutrient absorption occur in wheat grown in salt stress [ 46 ] It was also concluded that salt injury in plants is obvious in terms of decline in shoot growth, reduced leaf area, and decreased root zone [ 47 ]. A decrease in shoot branches, plant fresh and dry weight, and relative water content is reported in kiwi fruit under the influence of salt stress [ 48 ]. The deleterious impact of sodium and chloride ions is observed in brinjal grown under a soil-less system as well where a fewer number of leaves, yellowing of leaves due to salt, and decreased shoot growth are reported.…”

Responses of the Growth Characteristics of Spinach to Different Moisture Contents in Soil under Irrigation with Magnetoelectric Water

“…Ahmed et al reported that low concentrations of H 2 O 2 could induce the establishment of antioxidant defense mechanisms in crops [47]. Additionally, the use of exogenous H 2 O 2 could increase the tolerance of tomatoes, bell peppers, and watermelons to cold stress [48][49][50], improve the drought tolerance of soybeans, tobacco, and rapeseed [51][52][53], and improve the salt tolerance of wheat, tomatoes, and other crops [54][55][56][57]. Darmanti et al reported that under drought conditions, exogenous H 2 O 2 treatment enhanced the net photosynthetic rate and stomatal conductance of soybean leaves [58], while reducing the contents of the oxidative product malondialdehyde (MDA), which was induced by drought stress and H 2 O 2 .…”