Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions

Li, Lingyu; Yang, Haomeng; Ren, Weibo; Liu, Bei; Cheng, Dongmei; Wu, Xuezhuang; Gong, Jirui; Peng, Lianwei; Huang, Fang

doi:10.1007/s12374-016-0117-1

Cited by 10 publications

(14 citation statements)

References 36 publications

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“…Photosynthesis is highly sensitive to low-Pi stress because it requires Pi to express the genes and proteins involved in the process ( Zhang et al, 2014 , c ; Li L. et al, 2016 ). In the current study, low-Pi stress inhibited photosynthesis and biomass accumulation in melon seedlings.…”

Section: Discussionmentioning

confidence: 99%

Physiological and Biochemical Responses of Cucumis melo L. Chloroplasts to Low-Phosphate Stress

Weng

Zhang

et al. 2018

Front. Plant Sci.

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Phosphorus (P) is a limiting plant soil nutrient. Long-term low inorganic phosphate (Pi) irreversibly damages plant cells and hinders plant growth. Plants have evolved several adaptive biochemical, physiological, and developmental responses to low-Pi stress. However, little is known about chloroplast responses to low-Pi stress. In this study, we used physiological and biochemical analyses to investigate melon chloroplast responses to low-Pi stress. The results indicated that low-Pi stress impeded melon seedling growth and reduced its dry matter content by inhibiting the photosynthesis. Low-Pi stress reduced the P content in shoots, which inhibited ATP synthase (ATP-ase) activity, and disturbed the proton and electron transport efficiency on chloroplast photosynthetic electron transport chain. In addition, low-Pi stress induced reactive oxygen species (ROS) production in the leaves, which caused membrane peroxidation. Therefore, redox homeostasis was not maintained, and the melon leaves presented with symptoms of photooxidative stress. To mitigate photoinhibition, the melon plants initiated non-photochemical chlorophyll fluorescence quenching (NPQ) initiated by acidification of the thylakoid lumen to dissipate excess excitation energy, significantly improved ROS-scavenging enzyme activity. Based on these experimental results, we concluded that low Pi inhibited photosystem activity and caused photooxidative stress and photoinhibition. To alleviate these negative effects, the plant activated its NPQ mechanism, alternative electron transport pathways, and antioxidant system to protect its chloroplasts.

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Section: Discussionmentioning

confidence: 99%

Physiological and Biochemical Responses of Cucumis melo L. Chloroplasts to Low-Phosphate Stress

Weng

Zhang

et al. 2018

Front. Plant Sci.

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“…Seeds of L. chinensis (Trin.) Tzvel were germinated as described (Li et al, 2016). Two-week-old seedlings were transplanted to a vessel (350 mL) containing 1/4 strength Hoagland solution (Hoagland and Arnon, 1950) and cultured for an additional 4 weeks in a growth chamber with a light: dark cycle of 14:10 h (25:20°C) and light intensity and humidity was controlled at 280 μmol/m 2 s and 50%, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The solution was aerated via air bubbling and changed once a week. For stress treatment experiments, roots of the 4-week-old seedlings were carefully washed with fresh medium either containing repleted Pi (250 μM, control), low-Pi (2.5 μM) as described in the previous work (Li et al, 2016) or deprived-Pi (0 μM) and grown for additional 4 weeks in the controlled environment described above. In the low-Pi and deprived-Pi solutions, KH 2 PO 4 were substituted by an equimolar amount of K 2 SO 4 according to Wu et al (2003) and the solutions were replenished every 2 days.…”

Section: Methodsmentioning

confidence: 99%

“…Compared to other forage species, however, L. chinensis is more sensitive to ecosystem disturbance and stress conditions including water deficiency (Bai et al, 2004; Xu and Zhou, 2006). Therefore, it has been used as a popular model grass system for ecological as well as physiological research (Chen et al, 2013; Li et al, 2015, 2016; He et al, 2017). Based on the large-scale survey, leafy P content of land plants distributed in China is considerably lower than the global averages that excluded Chinese species.…”

Section: Introductionmentioning

confidence: 99%

“…Above-ground tissue biomass derived from photosynthesis is the major part contributing to grass productivity of L. chinensis . To understand how Pi starvation affects its above-ground tissue growth, we have initially investigated low-Pi effect on its photosynthetic properties and revealed for the first time a close correlation of photosystem II (PSII) accumulation and external Pi concentration in the medium (Li et al, 2016). The questions arise how Pi-deprivation affects above-ground tissue growth of L. chinensis and whether the correlation exists under Pi-deprived stress condition.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Study Reveals Insights of Sheepgrass (Leymus chinensis) Coping With Phosphate-Deprived Stress Condition

Yang

Peng

et al. 2019

Front. Plant Sci.

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Sheepgrass [ Leymus chinensis (Trin.) Tzvel] is a valuable forage plant highly significant to the grassland productivity of Euro-Asia steppes. Growth of above-ground tissues of L. chinensis is the major component contributing to the grass yield. Although it is generally known that this species is sensitive to ecosystem disturbance and adverse environments, detailed information of how L. chinensis coping with various nutrient deficiency especially phosphate deprivation (-Pi) is still limited. Here, we investigated impact of Pi-deprivation on shoot growth and biomass accumulation as well as photosynthetic properties of L. chinensis . Growth inhibition of Pi-deprived seedlings was most obvious and reduction of biomass accumulation and net photosynthetic rate (Pn) was 55.3 and 63.3%, respectively, compared to the control plants grown under Pi-repleted condition. Also, we compared these characters with seedlings subjected to low-Pi stress condition. Pi-deprivation caused 18.5 and 12.3% more reduction of biomass and Pn relative to low-Pi-stressed seedlings, respectively. Further analysis of in vivo chlorophyll fluorescence and thylakoid membrane protein complexes using 2D-BN/SDS-PAGE combined with immunoblot detection demonstrated that among the measured photosynthetic parameters, decrease of ATP synthase activity was most pronounced in Pi-deprived plants. Together with less extent of lipid peroxidation of the thylakoid membranes and increased ROS scavenger enzyme activities in the leaves of Pi-deprived seedlings, we suggest that the decreased activity of ATP synthase in their thylakoids is the major cause of the greater reduction of photosynthetic efficiency than that of low-Pi stressed plants, leading to the least shoot growth and biomass production in L. chinensis .

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Combined impact of heat stress and phosphate deficiency on growth and photochemical activity of sheepgrass (Leymus chinensis)

Yang

Liu

et al. 2018

Journal of Plant Physiology

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Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions

Cited by 10 publications

References 36 publications

Physiological and Biochemical Responses of Cucumis melo L. Chloroplasts to Low-Phosphate Stress

Physiological and Biochemical Responses of Cucumis melo L. Chloroplasts to Low-Phosphate Stress

Comparative Study Reveals Insights of Sheepgrass (Leymus chinensis) Coping With Phosphate-Deprived Stress Condition

Combined impact of heat stress and phosphate deficiency on growth and photochemical activity of sheepgrass (Leymus chinensis)

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