Physiologic effects of 2,3-DPG-depleted red cells with high affinity for oxygen

Valeri, C. R.; Collins, F. B.

doi:10.1152/jappl.1971.31.6.823

Cited by 57 publications

(14 citation statements)

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“…Consequently, it has been postulated that each of these conditions may be detrimental by ultimately causing tissue hypoxia. To It is noteworthy, that in a similar experiment involving human subjects, Valeri and Collins infused 3-5 U of ACD stored blood in eight anemic patients and noted no change in cardiac index or oxygen consumption as the oxyhemoglobin dissociation curve shifted from a mean P-, of 30.7-27.5 nmm Hg (7). However, in their study there was a significant increase in red cell mass and oxygen-carrying capacity with transfusion, that may have modified the effects of the acute change in oxyhemoglobin affinity.…”

Section: Discussionmentioning

confidence: 93%

Acute Changes in Oxyhemoglobin Affinity EFFECTS ON OXYGEN TRANSPORT AND UTILIZATION

Riggs¹,

Shafer²,

Guenter³

1973

J. Clin. Invest.

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Section: Discussionmentioning

confidence: 93%

Acute Changes in Oxyhemoglobin Affinity EFFECTS ON OXYGEN TRANSPORT AND UTILIZATION

Riggs¹,

Shafer²,

Guenter³

1973

J. Clin. Invest.

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“…Previous studies (4,16) have shown that tissue oxygenation, in presence of blood with high oxygen affinity and normal hematocrit, results in a lowering of W o n . In the present experiment, this was similarly observed at control hemoglobin levels.…”

Section: Discussionmentioning

confidence: 97%

Oxygenation in Anemic Newborn Lambs with High or Low Oxygen Affinity Red Cells

et al. 1981

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SummaryExperiments to determine the effect of the oxygen-hemoglobin dissociation curve on oxygen delivery during neonatal anemia, were carried out on 15 sedated and ventilated lambs less than 48 hr of age. Eight of the animals were exchange transfused with adult blood. The Pao of the exchange transfused group was 32.1 mm Hg (low Oz affinity) compared to 19.4 mm Hg for the controls (high 0 2 affinity). The animals were made anemic by isovolumic exchange transfusions with plasma. At different levels of hemoglobin defined as mild (8 mg/100 ml), moderate (6 mg/100 ml), and severe (4 mg/100 ml) anemia, tissue oxygenation, hemodynamic status, and blood gases were compared. Mixed venous POz was significantly lower in the high affinity group throughout the study. Cardiac output was significantly greater in the low affinity group during severe anemia. Oxygen consumption remained stable in the low affinity group, but decreased significantly in the high a f f i t y group when the anemia was severe. The data indicate that during severe anemia, blood with a high Po0 is more capable of adequately oxygenating tissues than that with a low PSO. SpeculationEarly preterm newborn infants may not adequately oxygenate their tissues during anemia because of their high oxygen a f f i t y red cells.An important adaptive mechanism during adult anemia is the shift to the right of the oxygen hemoglobin dissociation curve (15). Early preterm newborn infants have red cells with a relatively high oxygen affinity (3) and besides the numerous neonatal complications of prematurity these infants can also have a fall in hemoglobin level. It is apparent that a study was required to determine whether there could be obvious physiologic disadvantages of being anemic during a period of life when red cells have a high affinity for oxygen.In order to answer this question, studies were planned using the newborn lamb, within the first 48 hr of life, as an experimental model. The newborn lamb is born with a Pm which is similar to that of an early preterm newborn infant (18-19 mm Hg) (1) while the Pm of the adult sheep blood is considerably higher (3Ck38 mm Hg) (10). This study was designed to compare at different degrees of anemia, tissue oxygenation, hemodynamic status as well as blood gases in newborn lambs with either high or low oxygen affinity red cells. MATERIALS AND METHODSFifteen newborn lambs less than 48 hr old were included in the study. Eight lambs containing high oxygen affinity red cells were considered as the high 0 2 affiity group and seven lambs who underwent a two volume exchange transfusion (160 ml/kg) using freshly drawn maternal blood made up the low oxygen affiity group. The animals were prepared in conformance with the guiding principles in the care and use of animals as recommended by the Declaration of Helsinki. They were first sedated enough to be kept asleep with repeated subcutaneous injections of Diazepam (0.3 mg/kg every 20-30 min). To eliminate sensibility to pain during catheter position, lidocaine hydrochloride (2%) was used...

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“…In some of these states, such as anemia, hypoxemia, and cardiac failure, there is a decrease in blood oxygen affinity (1)(2)(3). Under other circumstances, such as occur after transfusion of stored blood, oxygen affinity is increased (4)(5)(6)(7). However, the importance of such changes on oxygen availability to cells is unknown.…”

Section: Introductionmentioning

confidence: 99%

Effect of Increased Blood Oxygen Affinity on Work Performance of Rats

Woodson¹,

Wranne²,

Detter³

1973

J. Clin. Invest.

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A B S T R A C T Influence of altered blood oxygen affinity on maximal performance ability was evaluated in trained rats exercising to exhaustion in a graded treadmill test. Modification of blood oxygen affinity was achieved both by 2,3-diphosphoglycerate depletion, accomplished by exposure of animals to C02 and by exchange transfusion with blood exposed to bisulfite or stored in acid citrate dextrose, and by carbamylation of hemoglobin, produced by exchange transfusion of blood incubated with potassium cyanate. A decrease in oxygen tension at half-saturation of hemoglobin (P5o) from 36 to 23 mm Hg produced a decrease in resting central venous oxygen pressure of about 12 mm Hg. During exercise it caused an average decrease in work performance of about 10%, which was equivalent to that performance decrement caused by a decrease in hemoglobin concentration of approximately 10%. When superimposed on anemia, this change in blood oxygen affinity again caused a similar decrease in performance over and above that due to anemia alone. A marked rightward shift of the in vivo oxygen dissociation curve during severe exercise may have compensated for the reduced in vitro P5o.

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Physiologic effects of 2,3-DPG-depleted red cells with high affinity for oxygen

Cited by 57 publications

References 0 publications

Acute Changes in Oxyhemoglobin Affinity EFFECTS ON OXYGEN TRANSPORT AND UTILIZATION

Acute Changes in Oxyhemoglobin Affinity EFFECTS ON OXYGEN TRANSPORT AND UTILIZATION

Oxygenation in Anemic Newborn Lambs with High or Low Oxygen Affinity Red Cells

Effect of Increased Blood Oxygen Affinity on Work Performance of Rats

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