Physiochemical Responses of Stevia rebaudiana Bertoni Subjected to Sodium Chloride (NaCl) Salinity and Exogenous Salicylic Acid Application

The current work sought to investigate the impact of two chemicals (NaCl and Brassinolide) on growth parameters, chlorophyll pigments in stevia shoots cultured under in vitro condition. For this, nodal explants of stevia were cultured for four weeks on MS medium supplemented with 0.2 mg l-1 BAP, Kinetin 0.2 mg l-1 and various concentrations of NaCl (0, 60, 70, 90, 120, 150 mM) and BL (0, 1, 2, 3 4, 5µM) for screening under in vitro condition. Explant treated with NaCl showed reduction with increasing salinity levels in all the studied parameters while explants treated with Brassinolide showed best results in all the studied parameters. From the results, it is concluded that the stevia plant was less impacted by 60mM and 70mM NaCl concentrations when compared to other concentrations of NaCl, but BL increased/improved all the examined parameters when compared to control; 1µM and 2µM concentrations of BL responded the best.

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“…While at lower salinity level (60 mM) reduction of 13.72% was observed. Similar findings were reported in stevia [5,24,25].…”

Section: Resultssupporting

confidence: 90%

Response of NaCl and Brassinolide in Stevia rebaudiana under in vitro Condition

Singh

Dwivedi²,

Sahni³

et al. 2022

IJPSS

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“…In addition, AMF inoculation showed a clear enhancement in plants under salt stress that resulted in an increase in photosynthetic pigments (Chl a, b, and T Chl) compared with stressed control (non-inoculated plants). Several studies have found that salinity treatments reduce chlorophyll concentrations in stevia (Janah et al 2021), pepper (Taffouo et al 2017, bean (Alzahrani et al 2019), and peppermint (Fathi et al 2020), which probably happens due to the decreasing of Mg 2+ uptake, an essential mineral element for chlorophyll biosynthesis (Hashem et al 2014). Similar results were found by Gerami et al (2020) in stevia plants subjected to salt stress.…”

Section: Discussionmentioning

confidence: 99%

“…This proportion might be up to 50% by the year 2050 as a result of climate change (Gopalakrishnan and Kumar 2020), especially in arid and semi-arid areas. Generally, salt stress causes a disturbance in plant physiological and biochemical activities (Singh 2015;Janah et al 2021). These events take place either directly through a reduction in the speed of plant growth performance and yield (Ilangumaran and Smith 2017), or either indirectly by generation of osmotic, ionic, and nutritional constraint effects on plants (Santander et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Under salt stress conditions, reactive oxygen species (ROS) producing systems can lead to the destruction of nucleic acid and can cause lipid peroxidation (Farhangi-Abriz and Torabian 2017; Gill and Tuteja 2010). In addition, plants dispose a scavenging ROS system, which implicates antioxidant enzymes such as polyphenol oxidase, peroxidase, superoxide dismutase, peroxidase, and catalase; this system may help plants to alleviate the adverse effects of salt stress (Abdel Latef et al 2021;Ait-El-Mokhtar et al 2020;Janah et al 2021).…”

Section: Introductionmentioning

confidence: 99%

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Arbuscular Mycorrhizal Fungi Mitigates Salt Stress Toxicity in Stevia rebaudiana Bertoni Through the Modulation of Physiological and Biochemical Responses

Janah

Meddich

Elhasnaoui

et al. 2021

J Soil Sci Plant Nutr

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Salinization of soil is a major problem affecting many plant physiological processes. The use of arbuscular mycorrhizal fungi (AMF) plays a key role in improving the growth and the crops yield. The aim of this study was to evaluate the combined effects of salt stress and AMF on agro-physiological and biochemical responses of Stevia rebaudiana Bertoni. The experiment was carried out in a completely randomized design with ten replicates under the green house conditions. Stevia plants were inoculated with AMF consortium (MC) or with Rhizophagus irregularis (Ri) on the presence (80 mM of NaCl) or the absence (0 mM of NaCl) of salt stress. The results showed that plant height, dry biomass of shoots and roots parts, mycorrhizal frequency and intensity, relative water content, chlorophyll fluorescence, stomatal conductance, chlorophyll a, and total chlorophyll were significantly reduced by 29%, 72%, 36%, 84%, 65%, 24%, 17%, 60%, 28.50%, and 40.9% respectively under salt stress. These changes were associated with a significant increase in antioxidant enzymes (activity of polyphenol oxidase, peroxidase, superoxide dismutase, and catalase), electrolyte leakage, lipid peroxidation, and hydrogen peroxide contents. The application of AMF (MC and Ri) improved growth and physiological parameters through rising the activities of antioxidant enzymes and reduction of the oxidative damage caused by the salt stress. The better results were recorded with MC-inoculated plants. These findings suggested that AMF could be an effective strategy to alleviate the adverse effects of salt stress on Stevia rebaudiana.

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“…Salinity and drought can induce oxidative stress of ROS accumulation by increasing the level of MDA and other aldehydes. Another experiments showed that salinity alone increased MDA in plants, but when SA treatment was applied with it, MDA levels were reduced and the risk of oxidative stress reduced [ 46 , 74 , 75 ]. In this study, SA significantly reduced lipid peroxidation under salinity stress in Damask rose.…”

Section: Resultsmentioning

confidence: 99%

Biochemical and molecular responses of Rosa damascena mill. cv. Kashan to salicylic acid under salinity stress

et al. 2022

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Background Today, salinity stress is one of the most important abiotic stresses in the world, because it causes damage to many agricultural products and reduces their yields. Oxidative stress causes tissue damages in plants, which occurs with the production of reactive oxygen species (ROS) when plants are exposed to environmental stresses such as salinity. Today, it is recommended to use compounds that increase the resistance of plants to environmental stresses and improve plant metabolic activities. Salicylic acid (SA), as an intracellular and extracellular regulator of the plant response, is known as one of these effective compounds. Damask rose (Rosa damascena Mill.) is a medicinal plant from the Rosaceae, and its essential oils and aromatic compounds are used widely in the cosmetic and food industries in the world. Therefore, considering the importance of this plant from both medicinal and ornamental aspects, for the first time, we investigated one of the native cultivars of Iran (Kashan). Since one of the most important problems in Damask rose cultivation is the occurrence of salinity stress, for the first time, we investigated the interaction of several levels of NaCl salinity (0, 4, 8, and 12 ds m− 1) with SA (0, 0.5, 1, and 2 mM) as a stress reducer. Results Since salinity stress reduces plant growth and yield, in this experiment, the results showed that the increase in NaCl concentration caused a gradual decrease in photosynthetic and morphological parameters and an increase in ion leakage. Also, increasing the level of salinity stress up to 12 ds m− 1 affected the amount of chlorophyll, root length and leaf total area, all of which reduced significantly compared to plants under no stress. However, many studies have highlighted the application of compounds that reduce the negative effects of stress and increase plant resistance and tolerance against stresses. In this study, the application of SA even at low concentration (0.5 mM) could neutralize the negative effects of salinity stress in the Rosa damascena. In this regard, the results showed that salinity increases the activity of antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) and the concentration of proline, protein and glycine betaine (GB). Overexpression of antioxidant genes (Ascorbate Peroxidase (APX), CAT, Peroxidase (POD), Fe-SOD and Cu-SOD) showed an important role in salt tolerance in Damascus rose. In addition, 0.5 mm SA increased the activity of enzymatic and non-enzymatic systems and increased salinity tolerance. Conclusions The change in weather conditions due to global warming and increased dryness contributes to the salinization of the earth’s surface soils. Therefore, it is of particular importance to measure the threshold of tolerance of roses to salinity stress and the effect of stress-reducing substances in plants. In this context, SA has various roles such as increasing the content of pigments, preventing ethylene biosynthesis, increasing growth, and activating genes involved in stress, which modifies the negative effects of salinity stress. Also, according to the results of this research, even in the concentration of low values, positive results can be obtained from SA, so it can be recommended as a relatively cheap and available material to improve production in saline lands.

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Physiochemical Responses of Stevia rebaudiana Bertoni Subjected to Sodium Chloride (NaCl) Salinity and Exogenous Salicylic Acid Application

Cited by 12 publications

References 65 publications

Response of NaCl and Brassinolide in Stevia rebaudiana under in vitro Condition

Response of NaCl and Brassinolide in Stevia rebaudiana under in vitro Condition

Arbuscular Mycorrhizal Fungi Mitigates Salt Stress Toxicity in Stevia rebaudiana Bertoni Through the Modulation of Physiological and Biochemical Responses

Biochemical and molecular responses of Rosa damascena mill. cv. Kashan to salicylic acid under salinity stress

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