Physics and Chemical, Immunoenzyme and Biochemical Indicators of Oral Fluid in Periodontitis in Patients With Rheumatoid Arthritis

Abstract

Infection with comorbid periodontitis due to transient bacteremia in patients with rheumatoid arthritis (RA) can lead to microbial colonization outside the mouth and increase systemic immune inflammation. Periodontitis-induced endotoxemia increases the sensitivity of RA patients to autoantigens by activating membrane proteins, pro-inflammatory enzymes, and cytokines. RA and periodontitis have similar osteoclasia, allelic genes and general imbalance in the state of the cytokine network, and carbamylation of proteins and the formation of extracellular traps of neutrophils are associated with simultaneous autoimmune imbalance in the combination of RA and periodontitis, but the listed pathogenetic mechanisms require further study.
 The purpose and objectives of the study: to study the properties of the oral fluid in periodontitis in patients with RA, to determine the clinical and pathogenetic significance of changes in physicochemical, enzyme immunoassay and biochemical parameters in the pathology of the oral cavity and joints, to compare the indicators with the nature of the gingival microbiota.
 Material and methods. The study included 173 RA patients aged 18-76 years (average 46 years), among whom there were 21% of men and 79% of women. The duration from the first manifestation of the disease averaged 10 years. The frequency of RA seropositivity for rheumatoid factor in the blood was 75%, and for antibodies to citrulline cyclic peptide was 73%. The І, ІІ and ІІІ degrees of disease activity, respectively, were stated in 26%, 44% and 30% of cases, and the ratio of I, II, III and IV stages of the pathological process was 1: 6: 5: 3.
 Results. We studied the physicochemical adsorption-rheological properties of the oral fluid (surface tension, viscoelasticity, relaxation), the levels of pro-inflammatory cytokines in it, the concentration of aminopeptide, peptide, nucleotide and chromatophore fractions of average weight molecules, the ratio with similar parameters in blood serum. To assess the number of aerobic and facultative anaerobic microorganisms vegetating on the gingival mucosa, the method of sterile paper discs was used. Compared with healthy people in the control group, 99% of the number of RA patients has an increase in the concentration of interleukin 1b in the oral fluid (22 times), 98% of tumoronecrotic factor a (7 times), 43% of the average increase in the average weight molecules (by 47%), at the same time, the parameters are associated with the presence of secondary Sjogren's syndrome, with the severity of the course of both articular syndrome, and periodontitis, which is accompanied by a longer relaxation time of oral washings (by 36%), but their lower interfacial activity (by 11%), the ratio of the tumor necrosis factor parameter a with the level of this pro-inflammatory cytokine in the blood (by 70%), and there are dispersion relationships of individual indicators of the oral fluid with the vegetation on the gums of bacterioids, bifidobacteria, Escherichia coli, megaspheres, moraxellas, neisseria, propionibacteria, proteus, pseudomones, sarcins and streptococcus.
 Conclusions: the obtained data dictate the need to develop new approaches to medical technology for individual pathogenetic therapy of chronic generalized periodontitis, taking into account its etiology in RA patients, identifying criteria that allow assessing the activity of the pathological process, predicting the nature of the course of the disease and the effectiveness of treatment measures.

The musculoskeletal system diseases in pregnant women with high infection risk and the single nucleotide rs1544410 polymorphism of the calcitriol receptor gene

“…This is confirmed by the presence of ribosomal RNA transcripts of Chlamydia trachomatis and C.pneumoniae, intestinal bacterial DNA and products of bacterial breakdown in synovial tissue and fluid. In reactive arthritis, the synthesis of anti-inflammatory cytokines is also disrupted, which leads to a decrease in the elimination of bacteria [19]. It is quite likely that a certain role in the genesis of chronic persistent infection in this group of pregnant women can be played by the deficiency of vitamin D, which we have discovered during the research.…”

“…In general, in the development of inflammatory and dege nerative joint diseases, including of an autoimmune nature, attention is paid to the influence of exo- and endogenous factors, which include lifestyle, bad habits, infection with viral-bacterial associations, genetic features, etc. [19].…”