Physicochemical studies on polydeoxyribonucleotides containing defined repeating nucleotide sequences

Wells, Robert D.; Larson, Jacquelynn E.; Grant, Robert C.; Shortle, Barbara E.; Cantor, Charles R.

doi:10.1016/0022-2836(70)90121-x

Cited by 547 publications

(217 citation statements)

References 56 publications

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“…The extinction coefficient at 260 nm (6550 M -1 cm -1 ) was used to determine the concentration in mononucleotide units. 25 Aqueous platinum solutions were made at room temperature and stored two weeks at pH 7.0 before using. Aliquots of DNA and 1 solutions were mixed and diluted to the desired concentration for platinum/DNA ratios (rb) ranging from 0.02 to 0.5 mol of Pt per mol of nucleotide.…”

Section: Methodsmentioning

confidence: 99%

cis-[PtCl₂(4,7-H-5-methyl-7-oxo[1,2,4]triazolo[1,5-a]pyrimidine)₂]: A Sterically Restrictive New Cisplatin Analogue. Reaction Kinetics with Model Nucleobases, DNA Interaction Studies, Antitumor Activity, and Structure−Activity Relationships

et al. 1998

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The formation and isolation of the antitumor drug cisplatin analogue cis- The reactivity of the complex to model nucleobases 9-ethylguanine (9-EtGH) and 1-methylcytosine (1-MeC) has been investigated by 1 H NMR spectroscopy at 45°C in aqueous media. The results show that 1 reacts slowly with 9-EtGH (t 1/2 ≈ 5 days) by displacement of Cl -, producing cis- ) 2 (9-EtGH-N 7 ) 2 ], which is similar to the major cross-link adduct of cisplatin with DNA. However, 1 gives no reaction with 1-MeC. This appears to be due to the lesser reactivity of 1-MeC and to competition between the cross-link reaction and dimerization of 1 to [Pt 2 (µ-mtpo-N 3 ,N 4 ) 4 ]. Circular dichroism studies of DNA in the presence of 1 show that the platinum complex reacts efficiently after 48 h at a optimum ratio of 0.25 Pt atom/mol of DNA nucleotide. These results and those obtained from reaction of 1 with 9-EtGH suggest that the platinum compound binds the N7 atoms of two guanines of the same strand, forming intrastrand cross-linked adducts. Chelation of DNA bases by 1 causes important conformational changes, bringing the guanines close together. The anticancer activity of complex 1 has been tested against the human cancer cell lines MCF-7 breast carcinoma and A121 ovarian carcinoma. Results indicate a moderate antitumor activity against breast carcinoma and a marked and selective cytotoxic effect against ovarian carcinoma.

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Section: Methodsmentioning

confidence: 99%

cis-[PtCl₂(4,7-H-5-methyl-7-oxo[1,2,4]triazolo[1,5-a]pyrimidine)₂]: A Sterically Restrictive New Cisplatin Analogue. Reaction Kinetics with Model Nucleobases, DNA Interaction Studies, Antitumor Activity, and Structure−Activity Relationships

et al. 1998

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“…Further studies by Bernardi and Timasheff 6, Wells et aZ. 7 have shown that DNA'sof same GC content but of different sequence exhibit different physico-chemical properties. This The most sophisticated interpretation of the variations of the DNA CD spectra due to base composition has been presented by Gray and Tinoco in 1970 8.…”

Section: Introductionmentioning

confidence: 99%

A simple method for the computation of first neighbour frequencies of DNAs from CD spectra

Marck

Guschlbauer²

1978

Nucl Acids Res

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A procediure for the computation of the first neighbour frequencies of DNA's is presented. This procedure is based on the first neighbour approximation of Gray and Tinoco. We show that the knowledge of all the ten elementary CD signals attached to the ten double stranded first neighbour configurations is not necessary. One can obtain the ten frequencies of an unknown DNA with the use of eight elementary CD signals corresponding to eight linearly independent polymer sequences. These signals can be extracted very simply from any eight or more CD spectra of double strafded DNA' s of known frequencies. The ten frequencies of a DNA are obtained by least square fit of its CD spectrum with these elementary signals. One advantage of this procedure is that it does not necessitate linear programming, it can be used with CD data digitalized using a large number of wavelengths, thus permitting an accurate resolution of the CD spectra. Under favorable case, the ten frequencies of a DNA (not used as input data) can be determined with an average absolute error < 2%. We have also observed that certain satellite DNA's, those of Drosophila viriZis and CalZinectes sapidus have CD spectra compatible with those of DNA's of quasi random sequence ; these satellite DNA's should adopt also the B-form in solution. INTRODUCTIONThe dependence of CD spectra on base composition has been extensively studied during the past ten years. Felsenfeld and Hirschmann1 have first investigated linear dependence of UV absorption spectra over GC content these authors have concluded to the insufficiency of such an hypothesis. ORD

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“…2 mg/mL) stock solution in appropriate buffers, the exact concentrations were established using absorbance at 257 nm for ct-DNA (ε = 6600 dm 3 mol -1 cm -1 ), 254 nm for poly(dG-dC)2 (ε = 8400 dm 3 mol -1 cm -1 ) and 262 nm for poly(dA-dT)2 (ε = 6600 dm 3 mol -1 cm -1 ) [20,21,22].…”

Section: Stock Solutionsmentioning

confidence: 99%

Redox-active and DNA-binding coordination complexes of clotrimazole

et al. 2015

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Electrochemical studies indicate that the Cu 2+ complex 9 readily reduces to the reactiveoxygen-species-generating Cu + , which oxidatively damages DNA. There is a subtle correlation between log P values, calculated electrostatic potentials, and cytotoxicity of the complexes. The extent of cell-nucleus DNA-metal adduct formation in the HeLa cervixuterine carcinoma cell line does not necessarily correlate with cytotoxicity, indicating that the nature of DNA lesions may be crucial to activity.

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Physicochemical studies on polydeoxyribonucleotides containing defined repeating nucleotide sequences

Cited by 547 publications

References 56 publications

cis-[PtCl₂(4,7-H-5-methyl-7-oxo[1,2,4]triazolo[1,5-a]pyrimidine)₂]: A Sterically Restrictive New Cisplatin Analogue. Reaction Kinetics with Model Nucleobases, DNA Interaction Studies, Antitumor Activity, and Structure−Activity Relationships

cis-[PtCl₂(4,7-H-5-methyl-7-oxo[1,2,4]triazolo[1,5-a]pyrimidine)₂]: A Sterically Restrictive New Cisplatin Analogue. Reaction Kinetics with Model Nucleobases, DNA Interaction Studies, Antitumor Activity, and Structure−Activity Relationships

A simple method for the computation of first neighbour frequencies of DNAs from CD spectra

Redox-active and DNA-binding coordination complexes of clotrimazole

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Physicochemical studies on polydeoxyribonucleotides containing defined repeating nucleotide sequences

Cited by 547 publications

References 56 publications

cis-[PtCl2(4,7-H-5-methyl-7-oxo[1,2,4]triazolo[1,5-a]pyrimidine)2]: A Sterically Restrictive New Cisplatin Analogue. Reaction Kinetics with Model Nucleobases, DNA Interaction Studies, Antitumor Activity, and Structure−Activity Relationships

cis-[PtCl2(4,7-H-5-methyl-7-oxo[1,2,4]triazolo[1,5-a]pyrimidine)2]: A Sterically Restrictive New Cisplatin Analogue. Reaction Kinetics with Model Nucleobases, DNA Interaction Studies, Antitumor Activity, and Structure−Activity Relationships

A simple method for the computation of first neighbour frequencies of DNAs from CD spectra

Redox-active and DNA-binding coordination complexes of clotrimazole

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Product

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cis-[PtCl₂(4,7-H-5-methyl-7-oxo[1,2,4]triazolo[1,5-a]pyrimidine)₂]: A Sterically Restrictive New Cisplatin Analogue. Reaction Kinetics with Model Nucleobases, DNA Interaction Studies, Antitumor Activity, and Structure−Activity Relationships

cis-[PtCl₂(4,7-H-5-methyl-7-oxo[1,2,4]triazolo[1,5-a]pyrimidine)₂]: A Sterically Restrictive New Cisplatin Analogue. Reaction Kinetics with Model Nucleobases, DNA Interaction Studies, Antitumor Activity, and Structure−Activity Relationships