Physicochemical properties of the DNA of herpes viruses

Bs, Graham; Ludwig, Hanns; Bronson, David L.; Benyésh-Melnick, Matilda; Biswal, Nrusingh C.

doi:10.1016/0005-2787(72)90469-8

Cited by 69 publications

(34 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If this represents an early stage in the expansion of the array rather than a later large scale deletion, then the lineage leading to VZV must have diverged first, from that leading to HSV and PRV. This is consistent with the fact that VZV has a genomic DNA of 46% G + C (Davison & Scott, 1986), very distinct from those of HSV-1 (68% G + C; McGeoch et al, 1988) and PRV (72% G + C; Graham et al, 1972). In the HSV and PRV lineages, duplications starting with the US7 gene would then give rise, directly or secondarily, to the US4 and US6 genes.…”

Section: Discussionsupporting

confidence: 81%

Evolutionary relationships of virion glycoprotein genes in the S regions of alphaherpesvirus genomes

McGeoch

1990

Journal of General Virology

View full text Add to dashboard Cite

The short region in the genome of herpes simplex virus type 1 contains a contiguous array of five genes (US4, US5, US6, US7 and US8) which encode known or proposed virion-surface glycoprotein species. Counterparts for certain of these have been described in the genomes of other alphaherpesviruses, namely herpes simplex virus type 2, pseudorabies virus and varicellazoster virus. Within each of the US4-, US6-and US7-related sets, the amino acid sequences are most conserved in a region containing several cysteine residues. Comparisons in this region among the three sets were carried out by first aligning three cysteine residues which were very similarly placed in each set, and a number of other similarities were then visible. It was concluded that the US4, US6 and US7 sets of genes are related, and thus have evolved by duplication and divergence. The US8-related sequences are distinct from the US4, US6 and US7 sequences, although possible signs of a distant relationship were detected. The US8 set contains two clusters ofcysteine residues, and the sequences around these show some similarity, which was interpreted as evidence for occurrence of an intramolecular duplication event.

show abstract

Section: Discussionsupporting

confidence: 81%

Evolutionary relationships of virion glycoprotein genes in the S regions of alphaherpesvirus genomes

McGeoch

1990

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…wt. of 100 x 106 and base composition 67 % G + C (Becker et al, 1968;Kieff et al, 1971;Graham et al, 1972). It comprises two unique sequence components of mol.…”

Section: Introductionmentioning

confidence: 99%

DNA Sequence Anaylysis of an Immediate-Early Gene Region of the Herpes Simplex Virus Type 1 Genome (Map Coordinates 0.950 to 0.978)

Murchie

McGeoch

1982

Journal of General Virology

113

125

View full text Add to dashboard Cite

SUMMARYThe nucleotide sequence of 4 kilobases of DNA from within the short region of the genome of herpes simplex virus type 1 has been determined. This portion of DNA contains the junctions of the terminal and internal inverted repeat sequence components with the unique sequence component and the 5' regions of the genes which encode the Vmw 12, Vmw 68 and Vmw 175 immediate-early polypeptides. The transcription and translation initiation sites of all three genes and the 5' and 3' boundaries of the Vmw 12 and Vmw 68 gene introns have been localized on the DNA sequence and shown to be flanked by sequences which resemble those found in similar positions in other eukaryotic genes. For the Vmw 12 and Vmw 68 genes the promoters, the 5'-non-coding regions of the mRNAs, and the introns lie within the terminal and internal inverted repeats respectively while the polypeptide-coding regions lie in the short unique component. The introns consist largely of tandemly reiterated copies of a 22-nucleotide sequence: the Vmw 12 gene intron contains seven of these and the Vmw 68 gene intron five. The Vmw 175 gene is located entirely within the short repeats, of which those regions sequenced here have the extremely high G + C content of 78 %, in marked contrast to the value of 66 % obtained for the adjacent region of the unique sequence component. Prediction of the complete amino acid sequence of the Vmw 12 polypeptide, accounting for a mol. wt. of 9830, and of the first 523 amino-terminal amino acids of the Vmw 175 polypeptide has been made from the DNA sequence. The polypeptide-coding region of the Vmw 175 gene has an average G + C content of 80% but nevertheless specifies a wide range of amino acid types because of the maximal assignment of G and C residues to codon third base positions.

show abstract

“…The chromosome of herpes simplex virus (HSV) is a linear, double-stranded DNA molecule of~100 X 106 daltons (1)(2)(3)(4). A preliminary report (5) presented evidence that HSV DNA, isolated by mild extraction procedures, could infect primary rabbit-kidney cells or rabbit-skin fibroblasts in the presence of diethylaminoethyl-dextran.…”

mentioning

confidence: 99%

Infectious DNA from Herpes Simplex Virus: Infectivity of Double-stranded and Single-stranded Molecules

Sheldrick¹,

Laithier²,

Lando³

et al. 1973

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The infectious units in native and alkalidenatured preparations of DNA of herpes simplex virus were characterized with respect to their sensitivity to Neurospora crassa endonuclease, their sedimentation properties in high-salt, neutral sucrose gradients, and their sensitivity to hydrodynamic shearing forces. Infectious molecules in native preparations were resistant to N. crassa endonuclease, sedimented at 56 S, and were highly sensitive to shearing forces. After alkaline denaturation, infectious molecules became sensitive to the N. crassa enzyme, sedimented at 200 S, and were relatively resistant to shear. We conclude that both intact duplex molecules (z100 X 106 daltons) and intact single strands (-50 X 106 daltons) are capable of initiating productive infection.The chromosome of herpes simplex virus (HSV) is a linear, double-stranded DNA molecule of~100 X 106 daltons (1-4). A preliminary report (5) presented evidence that HSV DNA, isolated by mild extraction procedures, could infect primary rabbit-kidney cells or rabbit-skin fibroblasts in the presence of diethylaminoethyl-dextran. The infectious unit was shown to be DNase-sensitive, resistant to anti-HSV serum, and to have a density in CsCl of 1.7 g/cm3. Additional studies (Sheldrick and Laithier, in preparation) have shown that infectivity is protease-resistant, and that progeny virus issuing from DNA-infected cells carry genetic markers present in the infecting DNA. In this communication, we describe experiments that further characterize the infectious unit in native preparations as a molecule of HSV DNA. Moreover, we present evidence that intact single strands (-50 X 106 daltons) of HSV DNA are also infectious. MATERIALS AND METHODSCulture Medium. Eagle's minimal essential medium (Eurobio, Paris) was supplemented with 3.2 g of glucose, 2.7 g of tryptose phosphate (Difco), and 2.0 g of NaHCO3 per liter. All incubations using this medium were performed in an atmosphere of 5% CO2.Cells. The strain of rabbit-skin fibroblasts, RS537, used in this study was isolated and kindly provided by Dr. G. Orth (6). The cells were propagated at 370 in medium supplemented with 10% calf serum in the absence of antibiotics. Penicillin (100 units/ml) and streptomycin (100 yg/ml) were added to Abbreviations: HSV, herpes simplex virus; PFU, plaque-forming units; PBS, phosphate-buffered saline (pH 7.0). 3621 the medium for the growth of virus and for plaque assays. Cultures were trypsinized twice weekly and reseeded at l/4-1/5 the original cell concentration. The experiments described here were performed with cells between their 40th and 70th passages.Virus. The strain of HSV (subtype 1) used here is designated A44 and has been described (7). It produces a syncytial cytopathic effect in RS537 cultures and, in this sense, is similar to the MP variant described by Hoggan and Roizman (8).Virus Growth Qnd Purification. Confluent cultures of RS537 cells in 1-liter Roux bottles (about 2 to 3 X 107 cells per bottle)were infected with 1 to 2 X 107 plaque-forming units (PFU) of virus i...

show abstract

Physicochemical properties of the DNA of herpes viruses

Cited by 69 publications

References 19 publications

Evolutionary relationships of virion glycoprotein genes in the S regions of alphaherpesvirus genomes

Evolutionary relationships of virion glycoprotein genes in the S regions of alphaherpesvirus genomes

DNA Sequence Anaylysis of an Immediate-Early Gene Region of the Herpes Simplex Virus Type 1 Genome (Map Coordinates 0.950 to 0.978)

Infectious DNA from Herpes Simplex Virus: Infectivity of Double-stranded and Single-stranded Molecules

Contact Info

Product

Resources

About