Physicochemical Properties of Chlorophylls in Oxygenic Photosynthesis — Succession of Co-Factors from Anoxygenic to Oxygenic Photosynthesis

Kobayashi, Masami; Akutsu, Shinya; Fujinuma, Daiki; HayatoFurukawa,; Komatsu, Hirokazu; Hotota, Yuichi; Kato, Yuki; YoshinoriKuroiwa,; Watanabe, Tadashi; Ohnishi‐Kameyama, Mayumi; HiroshiOno,; Ohkubo, Shuichi; Miyashita, Hideaki

doi:10.5772/55460

Cited by 26 publications

(51 citation statements)

References 71 publications

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“…NIES-2086NIES- , 2087NIES- , 2884 are shown in Fig. 3 Photosynth Res Gouterman et al 1963;Weiss 1978;Petke et al 1979;Hanson 1991;Kobayashi et al 2006Kobayashi et al , 2013. For example, replacement of the electron-donating group, /CH 3 , on ring II of Chl a by the electron-withdrawing group, ?CHO, yielding Chl b, causes a remarkable red-shift of the Soret band (ca.…”

Section: Normal-phase Hplcmentioning

confidence: 95%

Unique chlorophylls in picoplankton Prochlorococcus sp. “Physicochemical properties of divinyl chlorophylls, and the discovery of monovinyl chlorophyll b as well as divinyl chlorophyll b in the species Prochlorococcus NIES-2086”

et al. 2016

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In this review, we introduce our recent studies on divinyl chlorophylls functioning in unique marine picoplankton Prochlorococcus sp. (1) Essential physicochemical properties of divinyl chlorophylls are compared with those of monovinyl chlorophylls; separation by normal-phase and reversed-phase high-performance liquid chromatography with isocratic eluent mode, absorption spectra in four organic solvents, fluorescence information (emission spectra, quantum yields, and life time), circular dichroism spectra, mass spectra, nuclear magnetic resonance spectra, and redox potentials. The presence of a mass difference of 278 in the mass spectra between [M+H] and the ions indicates the presence of a phytyl tail in all the chlorophylls. (2) Precise high-performance liquid chromatography analyses show divinyl chlorophyll a' and divinyl pheophytin a as the minor key components in four kinds of Prochlorococcus sp.; neither monovinyl chlorophyll a' nor monovinyl pheophytin a is detected, suggesting that the special pair in photosystem I and the primary electron acceptor in photosystem II are not monovinyl but divinyl-type chlorophylls. (3) Only Prochlorococcus sp. NIES-2086 possesses both monovinyl chlorophyll b and divinyl chlorophyll b, while any other monovinyl-type chlorophylls are absent in this strain. Monovinyl chlorophyll b is not detected at all in the other three strains. Prochlorococcus sp. NIES-2086 is the first example that has both monovinyl chlorophyll b as well as divinyl chlorophylls a/b as major chlorophylls.

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Section: Normal-phase Hplcmentioning

confidence: 95%

Unique chlorophylls in picoplankton Prochlorococcus sp. “Physicochemical properties of divinyl chlorophylls, and the discovery of monovinyl chlorophyll b as well as divinyl chlorophyll b in the species Prochlorococcus NIES-2086”

et al. 2016

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“…The structure of chlorophyll d differs from that of chlorophyll a in the presence of a formyl group at position C-3 (Fig. 1 [27,28]. Isolation and fragmentation of the ion at m/z 873 in the ion trap gave rise to major ions at m/z 595 and m/z 535 in MS 2 , arising from loss of the phytyl chain from C-17 3 , and loss of the phytyl chain in addition to the CO 2 Me group from C-13 2 , respectively [27,28].…”

Section: Prochlorococcusmentioning

confidence: 99%

“…1 [27,28]. Isolation and fragmentation of the ion at m/z 873 in the ion trap gave rise to major ions at m/z 595 and m/z 535 in MS 2 , arising from loss of the phytyl chain from C-17 3 , and loss of the phytyl chain in addition to the CO 2 Me group from C-13 2 , respectively [27,28]. Further fragmentations can be discerned from the APCI multistage tandem mass spectra of methyl-pyrophaeophorbide d [23] (Fig.…”

Section: Prochlorococcusmentioning

confidence: 99%

“…Under soft ionisation conditions, chlorophyll f produces a protonated molecule at m/z 907, isobaric with that of chlorophyll b under both APCI [27,28] and ESI [33,38], whereas matrix assisted laser desorption ionization (MALDI) yielded M +. [32].…”

Section: Chlorophyll F-producing Cyanobacteriamentioning

confidence: 99%

“…Upon fragmentation, chlorophyll f gives rise to an MS 2 spectrum typical of a formyl substituted chlorophyll macrocycle [31], comprising an ion at m/z 879 corresponding to loss of 28 Da from [M+H] + . The base peak in MS 2 corresponded to an ion at m/z 629, arising from the loss of the phytyl chain [27,28,32,33], as commonly seen for phytol-esterified chlorophylls [10]. As [M+H] + of chlorophyll f is isobaric with that of chlorophyll b, and the fragmentations observed under MS n are typical of those of formyl substituted chlorophylls, LC/MS in combination with UV/vis detection is necessary to distinguish chlorophyll f and chlorophyll b.…”

Section: Chlorophyll F-producing Cyanobacteriamentioning

confidence: 99%

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Mass Spectrometry of Chlorophylls from Phototrophic Prokaryotes

Airs¹

2018

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Phototrophic prokaryotes inhabit a diverse array of environments on our planet and also contain a wide range of chlorophylls and bacteriochlorophylls, encompassing both chlorin and bacteriochorin structures. Mass spectrometry, particularly coupled with liquid chromatography and used in conjunction with soft ionization techniques, has been pivotal in the assignment of closely related chlorophyll and bacteriochlorophyll structures from these organisms. This review considers the ecological niches of the phototrophic prokaryotes, together with the mass spectrometric study of their chlorophyll and bacteriochlorophyll pigments.

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Surface and Temporal Biosignatures

Schwieterman¹

2018

Handbook of Exoplanets

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Recent discoveries of potentially habitable exoplanets have ignited the prospect of spectroscopic investigations of exoplanet surfaces and atmospheres for signs of life. This chapter provides an overview of potential surface and temporal exoplanet biosignatures, reviewing Earth analogues and proposed applications based on observations and models. The vegetation red-edge (VRE) remains the most well-studied surface biosignature. Extensions of the VRE, spectral 'edges' produced in part by photosynthetic or nonphotosynthetic pigments, may likewise present potential evidence of life. Polarization signatures have the capacity to discriminate between biotic and abiotic 'edge' features in the face of false positives from band-gap generating material. Temporal biosignaturesmodulations in measurable quantities such as gas abundances (e.g., CO 2 ), surface features, or emission of light (e.g., fluorescence, bioluminescence) that can be directly linked to the actions of a biosphere -are in general less well studied than surface or gaseous biosignatures. However, remote observations of Earth's biosphere nonetheless provide proofs of concept for these techniques and are reviewed here. Surface and temporal biosignatures provide complementary information to gaseous biosignatures, and while likely more challenging to observe, would contribute information inaccessible from study of the time-averaged atmospheric composition alone.

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Physicochemical Properties of Chlorophylls in Oxygenic Photosynthesis — Succession of Co-Factors from Anoxygenic to Oxygenic Photosynthesis

Abstract: Photosynthesis 48 Chl a Chl f Chl d Chl b 10 9 8 ppm Figure 13. 1 H-1 H-NOESY spectra of Chls a, b, d and f measured in acetone-d 6 at 273 K.

Cited by 26 publications

References 71 publications

Unique chlorophylls in picoplankton Prochlorococcus sp. “Physicochemical properties of divinyl chlorophylls, and the discovery of monovinyl chlorophyll b as well as divinyl chlorophyll b in the species Prochlorococcus NIES-2086”

Unique chlorophylls in picoplankton Prochlorococcus sp. “Physicochemical properties of divinyl chlorophylls, and the discovery of monovinyl chlorophyll b as well as divinyl chlorophyll b in the species Prochlorococcus NIES-2086”

Mass Spectrometry of Chlorophylls from Phototrophic Prokaryotes

Surface and Temporal Biosignatures

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