Physicochemical properties of a non-reducing maltoheptaose prepared by dual-enzyme cascade reaction from starch

Abstract

The existence of a reducing end in the structure of maltodextrin can limit its applications as undesirable Maillard reaction would occur in some food processing steps. Consequently, a non-reducing maltoheptaose (N-G7) with a single degree of polymerisation was prepared through a cascade reaction of cyclodextrinase and maltooligosyltrehalose synthase, using β-cyclodextrin as substrate. The physicochemical properties of N-G7 were investigated. N-G7 exhibited low moisture absorption ability (8.91 and 18.02% at 43 and 81% relative humidity, respectively), excellent pH stability and thermostability (less than 10% N-G7 was hydrolysed between pH 4 and 10, even at 100°C), and a melting point higher than that of maltodextrin, as well as a typical gel-like behaviour. Most importantly, the results of Maillard reaction indicated that N-G7 was considered to be non-reducing, which suggested that it could be used in food processing where Maillard reaction should be avoided. Overall, the present work may provide important implications for the development and application of N-G7 in food products.