Physicochemical properties, content, composition and partial least squares models of A. trifoliata seeds oil

Transcriptome Analysis and GC-MS Profiling of Key Fatty Acid Biosynthesis Genes in Akebia trifoliata (Thunb.) Koidz Seeds

“…A. trifoliata was cultivated in the experimental field of the Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences (Yuanjiang, Hunan province). GD-3 was selected for study because of its lower ASO content and higher seed yield compared with other varieties in this area [ 12 ]. According to our statistics, the fruit weight of GD-3 is generally less than 200 g, and its peel is purple when ripe.…”

“…First, a small grinder was used to smash the seeds, and the resulting powder was filtered through a 30-mesh sieve. Then, the Soxhlet extraction method was used to determine ASO content [ 12 ]. The conditions for Soxhlet extraction were: temperature 50 °C, solid to liquid ratio 1:60, and extraction time 4 h. After the determination of ASO content, the sample was collected in a 1.5 mL centrifuge tube and stored at 4 °C.…”

“…Before GC-MS analysis, FAs were converted to FA methyl esters (FAMEs). As described in previous studies [ 12 , 20 ], 0.06 g of ASO was diluted with diethyl ether/petroleum ether (1:1 v / v , 2 mL) and 0.4 M KOH-CH 3 OH (1 mL), vortexed, and maintained at room temperature (approximately 25 °C) for 2.5 h. Then, redistilled water (2 mL) was added to the mixture, which was then vortexed and centrifuged at 4500 rpm for 2 min. Finally, the organic phase containing FAMEs (100 mL) was collected and diluted with petroleum ether (900 mL).…”

“…Its seeds and pericarp are usually discarded, resulting in a waste of resources. However, the seeds contain a lot of oil and proteins and therefore have high processing value [ 12 , 13 ].…”

“…ASO is composed mainly of C16 and C18 FAs, such as palmitic acid and oleic acid [ 14 ]. Zhou et al showed that the Soxhlet extraction method was the most effective method for ASO extraction [ 15 ], and Zhong et al showed that a near-infrared spectroscopy model could accurately predict ASO content [ 12 ]. However, a few studies have been carried out on ASO biosynthetic pathways [ 16 ].…”