Physicochemical Properties and Skin Protection Activities of Polysaccharides from Usnea longissima by Graded Ethanol Precipitation

Abstract

Four Usnea longissima polysaccharides (ULPs; ULP15, ULP30, ULP50, and ULP70) were obtained from the lichen U. longissima via water extraction and graded ethanol precipitation. The obtained ULPs were all heteropolysaccharides with a few proteins, with which glucose was the major monosaccharide composition. With the increase in the precipitated ethanol concentrations, the content of galactose, xylose, and mannose increased, whereas that of glucose decreased. Moreover, the antioxidant activity test demonstrated that ULP15 exhibited better reducing power and stronger scavenging ability on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl free radicals. Importantly, ULP15 also had a better proliferative effect on human HaCaT keratinocytes and dermal fibroblasts. Meanwhile, ULP15 protected HaCaT keratinocytes from UVB-induced proliferation inhibition and exhibited tyrosinase inhibition activity. Therefore, this work provides interesting insight into the preparation of cosmetic ingredients using the polysaccharide ULP15.

Effect of Crude Polysaccharides from Ecklonia cava Hydrolysate on Cell Proliferation and Differentiation of Hanwoo Muscle Stem Cells for Cultured Meat Production

“…The cell-proliferative effects of polysaccharides are obtained from various materials [24,25]. Polysaccharides extracted from Ganoderma amboinense, a type of mushroom, have a proliferative effect on normal cells (293T/17, HUV-EC-C, NIH/3T3, and THP-1) [25].…”

“…Polysaccharides extracted from Ganoderma amboinense, a type of mushroom, have a proliferative effect on normal cells (293T/17, HUV-EC-C, NIH/3T3, and THP-1) [25]. Additionally, Yang et al [24] reported that polysaccharides obtained from Usnea longissima (moss) via ethanol precipitation significantly increased the proliferation of human Ha-CaT keratinocytes. In these studies, the authors explained that the antioxidant activity of polysaccharides had a positive effect on cell proliferation.…”

The Current State and Future Prospects of Auricularia auricula’s Polysaccharide Processing Technology Portfolio

“…However, if the polysaccharide concentration is too low, the recovery of the polysaccharide will be reduced and the consumption of ethanol will be greatly increased. Usually, the concentration of polysaccharide in the mixture is adjusted from 0.25% ( w / v ) to 3% ( w / v ) before using this method [ 47 , 48 , 49 , 50 ]. The concentration grading method is commonly used in the research and development of polysaccharide nutraceuticals because it is much easier than column chromatography.…”

Window on Bacillus maritimus MSM1 Exopolysaccharide Characterization and Possibility of its Anti-Alzheimer Property in In-Vitro Model

“…Tyrosinase inhibitors are medications, which can suppress the tyrosinase activity. Several polysaccharides from natural origin showed anti-tyrosinase activity as the fruit's pericarp polysaccharides, longan (Yang et al 2008), Usnea longissima polysaccharides (Yang et al 2021).…”