Physicochemical Properties and Isoenzyme Composition of Hexokinase From Normal and Malignant Human Tissues

Monakhov, N. K.; Neistadt, E. L.; Shavlovskii, M. M.; Shvartsman, A.; Neifakh, S. A.

doi:10.1093/jnci/61.1.27

Cited by 103 publications

(25 citation statements)

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“…In 1978 Monakhov et al (29) reported on the fact that hexokinase played an important role in this process. It was found that in human malignant tissue the slower isoenzymes in the hexokinase spectrum showed a much lower abundance.…”

Section: Trapping and Metabolism Of Glucose And Deoxyglucosementioning

confidence: 99%

FDG Accumulation and Tumor Biology

Pauwels

Ribeiro

Stoot

et al. 1998

Nuclear Medicine and Biology

315

190

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ABSTRACT. The tumoral uptake of fluorine-18-deoxyglucose (FDG) is based upon enhanced glycolysis. Following injection, FDG is phosphorylated and trapped intracellularly. An important mechanism to transport FDG into the transformed cell is based upon the action of glucose transporter proteins; furthermore, highly active hexokinase bound to tumor mitochondria helps to trap FDG into the cell. In addition, enhanced FDG uptake may be due to relative hypoxia in tumor masses, which activates the anaerobic glycolytic pathway. In spite of these processes, FDG uptake is relatively aspecific since all living cells need glucose. Clinical use is therefore recommended in carefully selected patients. NUCL MED BIOL 25;4:317-322, 1998.

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Section: Trapping and Metabolism Of Glucose And Deoxyglucosementioning

confidence: 99%

FDG Accumulation and Tumor Biology

Pauwels

Ribeiro

Stoot

et al. 1998

Nuclear Medicine and Biology

315

190

View full text Add to dashboard Cite

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“…In man, overexpression of GLUT1 and GLUT3 has been observed in several tumour types, including cerebral tumours, hepatocellular carcinoma and pancreatic tumours [25][26][27][28]. Furthermore, an increase in the activity of several enzymes controlling the glycolytic pathway has been demonstrated (hexokinase, phosphofructokinase, pyruvate dehydrogenase) [29]. For instance, several isoenzymes of hexokinase have been observed in the Novikoff ascites tumour [30].…”

Section: Biological Characteristics Of 18-fdgmentioning

confidence: 99%

Oncological applications of positron emission tomography with fluorine-18 fluorodeoxyglucose

Rigo¹,

Paulus²,

et al. 1996

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Abstract. Positron emission tomography (PET) is now primarily used in oncological indication owing to the successful application of fluorine-18 fluorodeoxyglucose (FDG) in an increasing number of clinical indications at different stages of diagnosis, and for staging and followup. This review first considers the biological characteristics of FDG and then discusses methodological considerations regarding its use. Clinical indications are considered, and the results achieved in respect of various organs and tumour types are reviewed in depth. The review concludes with a brief consideration of the ways in which clinical PET might be improved.

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“…Once glucose reaches the intracellular space and the mitochondria, it is phosphorylated by hexokinase. Tumor hexokinase is, however, qualitatively (i.e., its isoenzyme composition) [14], quantitatively [23], and vis-a-vis its intracellular location [2] different from the hexokinase of normal tissues, e.g., of the kidney. On the other hand, no such variability has been found concerning G6Pase in tumor cells [4].…”

Section: But the O T H E R Enzymes Acting On Glucose 6-p H O S P H A mentioning

confidence: 99%

Tumor localization with18F-2-fluoro-2-deoxy-D-glucose: Comparative autoradiography, glucose 6-phosphatase histochemistry, and histology of renally implanted sarcoma of the rat

Paul¹,

Johansson

Kellokumpu‐Lehtinen

et al. 1985

Res. Exp. Med.

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Rous sarcoma cells were implanted into the kidney of rats. After 5 days of growth the renal tumor was used for comparing histology with glucose 6-phosphatase (G6Pase) enzyme histochemistry (EHC) and 18F-fluoro-2-deoxyglucose (FDG) auroradiography (ARG). It was found that the regions of the kidney tumor that had retained normal kidney structures were devoid of FDG, whereas there was histochemical staining of normal cortical areas. Regions of tumor growth, on the other hand, retained FDG and lacked G6Pase. Necrotic areas did not accumulate FDG. There was a dramatic decrease in the areas of G6Pase activity as a result of tumor infiltration in the kidney. The results show that FDG, currently being evaluated as a tumor detecting radiopharmaceutical indeed accumulates into areas of vital malignant growth, and they indicate that FDG positron emission tomographic (PET) images reveal the true anatomic location of malignant tissue.

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Physicochemical Properties and Isoenzyme Composition of Hexokinase From Normal and Malignant Human Tissues

Cited by 103 publications

References 0 publications

FDG Accumulation and Tumor Biology

FDG Accumulation and Tumor Biology

Oncological applications of positron emission tomography with fluorine-18 fluorodeoxyglucose

Tumor localization with18F-2-fluoro-2-deoxy-D-glucose: Comparative autoradiography, glucose 6-phosphatase histochemistry, and histology of renally implanted sarcoma of the rat

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