Physicochemical properties and in vitro toxicity of cationic liposome cDNA complexes

Schreier, Hans; Gagné, Lucie; Bock, Thomas; Erdos, Gregory W.; Druzgala, Pascal; Conary, Jon T.; Müller, Bernd W.

doi:10.1016/s0031-6865(97)00019-8

Cited by 40 publications

(24 citation statements)

References 15 publications

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“…(C) DNA complexed with cationic lipids is protected from nucleases and is inaccessible to DNA binding dyes. The protective role of the lipid is dependent on the charge ratio [48]. (D) Cationic lipids are usually formulated as liposomes using co-lipids such as DOPE and cholesterol.…”

Section: Summary Of the Basic Observations In Lipid-mediated Transfecmentioning

confidence: 99%

Cell Biological and Biophysical Aspects of Lipid-mediated Gene Delivery

Rao

Gopal

2006

Bioscience Reports

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Cationic lipids are conceptually and methodologically simple tools to deliver nucleic acids into the cells. Strategies based on cationic lipids are viable alternatives to viral vectors and are becoming increasingly popular owing to their minimal toxicity. The first-generation cationic lipids were built around the quaternary nitrogen primarily for binding and condensing DNA. A large number of lipids with variations in the hydrophobic and hydrophilic region were generated with excellent transfection efficiencies in vitro. These cationic lipids had reduced efficiencies when tested for gene delivery in vivo. Efforts in the last decade delineated the cell biological basis of the cationic lipid gene delivery to a significant detail. The application of techniques such as small angle X-ray spectroscopy (SAXS) and fluorescence microscopy, helped in linking the physical properties of lipid:DNA complex (lipoplex) with its intracellular fate. This biological knowledge has been incorporated in the design of the second-generation cationic lipids. Lipid-peptide conjugates (peptoids) are effective strategies to overcome the various cellular barriers along with the lipoplex formulations methodologies. In this context, cationic lipid-mediated gene delivery is considerably benefited by the methodologies of liposome-mediated drug delivery. Lipid mediated gene delivery has an intrinsic advantage of being a biomimetic platform on which considerable variations could be built to develop efficient in vivo gene delivery protocols.

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Section: Summary Of the Basic Observations In Lipid-mediated Transfecmentioning

confidence: 99%

Cell Biological and Biophysical Aspects of Lipid-mediated Gene Delivery

Rao

Gopal

2006

Bioscience Reports

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“…1 Problems associated with immunogenicity, the development of replication-competent viruses, and potential oncogenic activity have encouraged the development of safe and effective nonviral gene transfer systems. [2][3][4][5] Cationic lipids are widely used for in vivo gene transfer because of their ease of use and high in vitro transfection efficiency. 1,6,7 Intratumoral (i.t.)…”

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confidence: 99%

Studies of direct intratumoral gene transfer using cationic lipid-complexed plasmid DNA

et al. 2000

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Cationic lipid-mediated gene transfer is a safe and effective means of delivering potent immunomodulatory cytokines directly into tumors. This approach avoids undesirable side effects, including systemic toxicities. To investigate key factors affecting intratumoral (i.t.) gene transfer, cationic lipid-DNA complexes were injected into subcutaneous human melanoma tumors in severe combined immunodeficient mice. Animals received i.t. injections of VR1103, a DNA plasmid encoding the gene for human interleukin-2 (IL-2), either alone or complexed with the cationic lipid N-(1-(2,3-dimyristyloxypropyl)-N,N-dimethyl-(2-hydroxyethyl) ammonium bromide/dioleoyl phosphatidylethanolamine (DMRIE/DOPE). Tumors were subcultured and supernatants were tested for IL-2 secretion by enzyme-linked immunosorbent assay. IL-2 secretion was consistently higher when lipid:DNA (L:D) complexes were formulated at high L:D ratios (wt/wt), and IL-2 transgene expression increased in a DNA dose-dependent manner. A comparison of naked plasmid and lipid-complexed DNA revealed that lipid complexes were more effective for i.t. gene transfer. Using an enhanced green fluorescent protein reporter plasmid and flow cytometry, i.t. transfection efficiency was 1.74% (Ϯ 1.08%). Tumor injection technique, including injection volume and location, had a limited impact on i.t. gene transfer. These results indicate that the formulation and dosage of cationic L:D complexes, but not injection technique, play a key role in determining the level of i.t. transgene expression. Cancer Gene Therapy (2000) 7, 853-860

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“…144 Liposomes are prepared for inhalation either in liquid or dry powder form. 145 During nebulization, an amount of the formulation is lost and hence a manipulation of the lipid composition, and size and operating conditions are necessary to minimize the loss. [146][147][148][149] The dry powder liposome formulations are produced by lyophilization followed by milling or by spray-drying.…”

Section: Inhaled Particle Carriers and Strategies Liposomesmentioning

confidence: 99%

Inhaled chemotherapy in lung cancer: future concept of nanomedicine

Zarogoulidis

Chatzaki²,

Porpodis³

et al. 2012

IJN

166

160

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Regional chemotherapy was first used for lung cancer 30 years ago. Since then, new methods of drug delivery and pharmaceuticals have been investigated in vitro, and in animals and humans. An extensive review of drug delivery systems, pharmaceuticals, patient monitoring, methods of enhancing inhaled drug deposition, safety and efficacy, and also additional applications of inhaled chemotherapy and its advantages and disadvantages are presented. Regional chemotherapy to the lung parenchyma for lung cancer is feasible and efficient. Safety depends on the chemotherapy agent delivered to the lungs and is dose-dependent and time-dependent. Further evaluation is needed to provide data regarding early lung cancer stages, and whether regional chemotherapy can be used as neoadjuvant or adjuvant treatment. Finally, inhaled chemotherapy could one day be administered at home with fewer systemic adverse effects.

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Physicochemical properties and in vitro toxicity of cationic liposome cDNA complexes

Cited by 40 publications

References 15 publications

Cell Biological and Biophysical Aspects of Lipid-mediated Gene Delivery

Cell Biological and Biophysical Aspects of Lipid-mediated Gene Delivery

Studies of direct intratumoral gene transfer using cationic lipid-complexed plasmid DNA

Inhaled chemotherapy in lung cancer: future concept of nanomedicine

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