Physicochemical properties and cytotoxicity of cysteine-functionalized silver nanoparticles

Oćwieja, Magdalena; Barbasz, Anna; Walas, Stanisław; Roman, Maciej; Paluszkiewicz, C.

doi:10.1016/j.colsurfb.2017.09.042

Cited by 32 publications

(26 citation statements)

References 53 publications

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“…Previously described positively charged cysteinestabilized AgNPs, characterized by comparable size distribution as the nanoparticles investigated in this work, also exhibited lower impact on the viability of U-937 and HL-60 cells (Oćwieja et al 2017) than HEXAgNPs and CITAgNPs in the presence of HEX (Fig. 3a and Fig.…”

Section: Discussionsupporting

confidence: 68%

“…Based on these findings one can hypothesize that not nanoparticle charge but stabilizers coating them play a pivotal role in mechanisms of their toxicity. Nevertheless, it is important to bear in mind that more often thanks to the chemical reactions which take place with the participation of the stabilizers (e.g., protonation), the metal nanoparticles are charged (Oćwieja et al 2017).…”

Section: Discussionmentioning

confidence: 99%

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Sodium hexametaphosphate–induced enhancement of silver nanoparticle toxicity towards leukemia cells

Oćwieja

Barbasz

2020

J Nanopart Res

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Synergistic effects occurring between biologically active substances are of great importance for efficient treatment of many diseases. Therefore, the aim of research was to determine impact of sodium hexametaphosphate (HEX), which is a well-known permeabilizer, on the cytotoxicity of silver ions and two types of AgNPs towards HL-60 and U-937 tumor cells. The AgNPs were synthesized in a chemical reduction method using sodium borohydride and trisodium citrate (CITAgNPS) or sodium hypophosphite and HEX (HEXAgNPs). Imaging with the use of transmission electron microscopy (TEM) and atomic force microscopy (AFM) revealed that the AgNPs exhibited spherical shape and comparable size distribution. Electrophoretic mobility studies showed that the AgNPs were negatively charged. The mitochondrial and antioxidant activity as well as membrane lipid peroxidation and integrity after dose-dependent AgNP treatment were evaluated using biochemical assays. The impact of HEXAgNPs on the membrane integrity and inactivation of antioxidant enzymes of the cells was much higher than this one observed for CITAgNPs and silver ions of the same concentration. The membrane damage occurred as a result of lipid peroxidation which was induced by pure HEX and HEXAgNPs. It was also observed that HEX significantly increased cell membrane damage induced by CITAgNPs and silver ions although the cells exhibited different sensitivity to these components. Moreover, it was found that HEX can induce oxidative stress. Hence, it was revealed that HEX enhances AgNP activity when it is applied both as their stabilizer or supplement in their suspensions.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Sodium hexametaphosphate–induced enhancement of silver nanoparticle toxicity towards leukemia cells

Oćwieja

Barbasz

2020

J Nanopart Res

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“…The plates were air-dried. The bound stain was eluted with 100 μL trizma base, and the absorbance was read at 492nm using a microplate reader (Sunrise-basic, Tecan, Austria) [24]. Finally, the relative growth rate (RGR) was calculated by the following equation: RGR %=[italicAbsorbance italicof italicthe italictest italicsampleitalicAbsorbance italicof italicthe italiccontrol]×100…”

Section: Methodsmentioning

confidence: 99%

Wound Dressings Based on Chitosan-Dialdehyde Cellulose Nanocrystals-Silver Nanoparticles: Mechanical Strength, Antibacterial Activity and Cytotoxicity

Dong

2018

Polymers

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The present work envisages a simple approach to synthesize a new wound dressing based on chitosan-dialdehyde cellulose nanocrystal-silver nanoparticles (CS-DCNC-AgNPs). Silver nanoparticles (AgNPs) were generated in-situ by periodate oxidation of cellulose nanocrystals to generate aldehyde functions, which were used to reduce Ag + into Ag 0 in mild alkaline conditions. Subsequently, the dialdehyde cellulose nanocrystal-silver nanoparticles (DCNC-AgNPs) were added to chitosan (CS) to form the wound dressings by solution casting method. The aim was to enhance the antibacterial effect of CS by incorporation of AgNPs and to improve the mechanical strength and hydrophobicity of CS by incorporation of DCNC that cross-linked by hydrogen bonds. The antibacterial activities were evaluated against five gram-negative bacteria, one gram-positive bacteria, and three fungi. The in vitro cytotoxicity assay was performed using the NIH3T3 cell lines by Sulforhodamine B assay. Research outputs signified that CS-DCNC-AgNPs possessed good mechanical strength and hydrophobicity, high antibacterial activity and less cytotoxicity. Our results propose that CS-DCNC-AgNPs can be a promising, safe antibacterial to be incorporated in wound dressings.

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“…Nanoparticles stabilized by inorganic low molar mass anions are usually negatively charged. AgNPs capped by organic compounds having moieties capable of protonation and deprotonation, such as amino acids, can be negatively or positively charged (Oćwieja et al, 2017). As various scientific reports have shown, the surface charge of AgNPs affects the formation of protein corona around nanoparticles (Durán et al, 2015), as well as their further interactions with the cell membrane (Fröhlich, 2012).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant‐modulated cytotoxicity of silver nanoparticles

Barbasz

Oćwieja

Piergies

et al. 2021

J of Applied Toxicology

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The properties of silver nanoparticles (AgNPs) synthesized using compounds exhibiting biological activity seem to constitute an interesting issue worthy of examination. In these studies, two types of AgNPs were synthesized by a chemical reduction method using well‐known antioxidants: gallic acid (GA) and ascorbic acid (AA). Transmission electron microscopy (TEM) and atomic force microscopy (AFM) revealed that the AgNPs were spherical. The average size was equal to 26 ± 6 nm and 20 ± 7 nm in the case of ascorbic acid‐silver nanoparticles (AAgNPs) and gallic acid‐silver nanoparticles (GAAgNPs), respectively. Surface‐enhanced Raman spectroscopy (SERS) confirmed that the AgNPs were not stabilized by pure forms of applied antioxidants. Changes in mitochondrial activity and secretion of inflammatory and apoptosis mediators after the exposure of human promyelocytic (HL‐60) and histiocytic lymphoma (U‐937) cells to the AgNPs were studied to determine the impact of stabilizing layers on nanoparticle toxicity. The GAAgNPs were found to be more toxic for the cells than the AAgNPs. Their toxicity was manifested by a strong reduction in mitochondrial activity and induction of the secretion of interleukin‐6 (IL‐6), tumor necrosis factor‐α (TNF‐α), and caspase‐9. The addition of pure antioxidants to the AgNP suspensions was found to influence their toxicity. There was a significant positive effect in the case of the mixture of AA with AAgNPs and GA with GAAgNPs. The results obtained suggest that the presence of stabilizing agents adsorbed on the surface of AgNPs is the main factor in shaping their toxicity. Nevertheless, the toxic effect can be also tuned by the introduction of free antioxidant molecules to the AgNP suspensions.

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Physicochemical properties and cytotoxicity of cysteine-functionalized silver nanoparticles

Cited by 32 publications

References 53 publications

Sodium hexametaphosphate–induced enhancement of silver nanoparticle toxicity towards leukemia cells

Sodium hexametaphosphate–induced enhancement of silver nanoparticle toxicity towards leukemia cells

Wound Dressings Based on Chitosan-Dialdehyde Cellulose Nanocrystals-Silver Nanoparticles: Mechanical Strength, Antibacterial Activity and Cytotoxicity

Antioxidant‐modulated cytotoxicity of silver nanoparticles

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