Physicochemical, Pharmacologic, and in Vitro Cellular Effects of Loading Collagen Membranes with Zoledronic Acid

Abstract

This assignment applies to all translations of the Work as well as to preliminary display/posting of the abstract of the accepted article in electronic form before publication. If any changes in authorship (order, deletions, or additions) occur after the manuscript is submitted, agreement by all authors for such changes must be on file with the Publisher. An author's name may be removed only at his/her written request. (Note: Material prepared by employees of the US government in the course of their official duties cannot be copyrighted.

Release kinetics of prolyl hydroxylase inhibitors from collagen barrier membranes

“…Although collagen barrier membranes were proposed as carriers for zoledronate and growth factors due to their net effects, studies assessing the release kinetic are limited to antibiotics and zoledronate. [20][21][22] Our observations are in line with the burst release of zoledronate from 1 and 2 layered collagen barrier membranes and data from prolyl hydroxylase, vitamins, and growth factors that were adsorbed onto bone substitute materials. 20,23,24 However, the membranes release prolyl hydroxylase inhibitors within the first 6 hours which is faster than found with vitamins and growth factors on bone substitute materials.…”

“…[20][21][22] Our observations are in line with the burst release of zoledronate from 1 and 2 layered collagen barrier membranes and data from prolyl hydroxylase, vitamins, and growth factors that were adsorbed onto bone substitute materials. 20,23,24 However, the membranes release prolyl hydroxylase inhibitors within the first 6 hours which is faster than found with vitamins and growth factors on bone substitute materials. 23,24 However, this release kinetic is in line with the data from zoledronate from collagen barrier membranes where the highest release is found within the first 6 h. 20 That only one dose of prolyl hydroxylase inhibitors was assessed is a clear limitation of this study.…”

“…Studies with the double-layered BioGide Õ and the single-layered collagen barrier membrane BME-10X composed of bovine collagen I showed no difference regarding the release of bisphosphonates. 20 These data suggest that it is unlikely that monolayered collagen membranes show a different release kinetic.…”

Release kinetics and mitogenic capacity of collagen barrier membranes supplemented with secretome of activated platelets - the in vitro response of fibroblasts of the periodontal ligament and the gingiva

“…CBM with a different structure might show different release kinetics. Comparison of BioGide® with the single-layered CBM BME-10X composed of bovine collagen I revealed different release kinetics of bisphosphonates from these membranes [4]. Clearly, platelets release other factors besides PDGF-BB and TGFβ1 that induce mitogenic effects, including other PDGF isoforms such as PDGF-AB and other signalling molecules [37].…”

“…When healing is compromised, therapeutic approaches based on biologicals can support regeneration. CBM represent a promising carrier for bioactive molecules that stimulate the healing capacity as they are placed in the interface between soft and hard tissue defects where they can release factors and pharmaceuticals to target cells of both tissues [4, 5]. Platelets have been investigated for their capacity to stimulate oral tissue regeneration based on their key role in the wound healing cascade and their capacity to modulate inflammation [6–9].…”