“…Among composites, only CaDHA/TiNT composites showed hemolysis higher than 5% at the highest concentration applied. This indicates that the compatibility of TiNMs were improved in CaDHA/TiNM, which is in agreement with literature data that have shown that nanosized and/or nanoporous HAP is a highly hemocompatible material [84,87,88].…”
Section: Hemocompatibility Of Tinms and Cap/tinms Compositessupporting
The constantly growing need for advanced bone regeneration materials has motivated the development of calcium phosphates (CaPs) composites with a different metal or metal-oxide nanomaterials and their economical and environmentally friendly production. Here, two procedures for the synthesis of CaPs composites with TiO2 nanoplates (TiNPl) and nanowires (TiNWs) were tested, with the immersion of TiO2 nanomaterials (TiNMs) in corrected simulated body fluid (c-SBF) and precipitation of CaP in the presence of TiNMs. The materials obtained were analyzed by powder X-ray diffraction, spectroscopic and microscopic techniques, Brunauer–Emmett–Teller surface area analysis, thermogravimetric analysis, dynamic and electrophoretic light scattering, and their hemocompatibility and ability to induce reactive oxygen species were evaluated. After 28 days of immersion in c-SBF, no significant CaP coating was formed on TiNMs. However, the composites with calcium-deficient apatite (CaDHA) were obtained after one hour in the spontaneous precipitation system. In the absence of TiNMs, CaDHA was also formed, indicating that control of the CaP phase formed can be accomplished by fine-tuning conditions in the precipitation system. Although the morphology and size of crystalline domains of CaDHA obtained on the different nanomaterials differed, no significant difference was detected in their local structure. Composites showed low reactive oxygen species (ROS) production and did not induce hemolysis. The results obtained indicate that precipitation is a suitable and fast method for the preparation of CaPs/TiNMs nanocomposites which shows great potential for biomedical applications.
“…Among composites, only CaDHA/TiNT composites showed hemolysis higher than 5% at the highest concentration applied. This indicates that the compatibility of TiNMs were improved in CaDHA/TiNM, which is in agreement with literature data that have shown that nanosized and/or nanoporous HAP is a highly hemocompatible material [84,87,88].…”
Section: Hemocompatibility Of Tinms and Cap/tinms Compositessupporting
The constantly growing need for advanced bone regeneration materials has motivated the development of calcium phosphates (CaPs) composites with a different metal or metal-oxide nanomaterials and their economical and environmentally friendly production. Here, two procedures for the synthesis of CaPs composites with TiO2 nanoplates (TiNPl) and nanowires (TiNWs) were tested, with the immersion of TiO2 nanomaterials (TiNMs) in corrected simulated body fluid (c-SBF) and precipitation of CaP in the presence of TiNMs. The materials obtained were analyzed by powder X-ray diffraction, spectroscopic and microscopic techniques, Brunauer–Emmett–Teller surface area analysis, thermogravimetric analysis, dynamic and electrophoretic light scattering, and their hemocompatibility and ability to induce reactive oxygen species were evaluated. After 28 days of immersion in c-SBF, no significant CaP coating was formed on TiNMs. However, the composites with calcium-deficient apatite (CaDHA) were obtained after one hour in the spontaneous precipitation system. In the absence of TiNMs, CaDHA was also formed, indicating that control of the CaP phase formed can be accomplished by fine-tuning conditions in the precipitation system. Although the morphology and size of crystalline domains of CaDHA obtained on the different nanomaterials differed, no significant difference was detected in their local structure. Composites showed low reactive oxygen species (ROS) production and did not induce hemolysis. The results obtained indicate that precipitation is a suitable and fast method for the preparation of CaPs/TiNMs nanocomposites which shows great potential for biomedical applications.
“…Although the vascular grafts containing 20% DIP showed a higher hemolysis percentage, there was no significance difference with respect to the blank PCL ( p > 0.05). Samples displaying a hemolysis percentage lower than 5% have been defined as hemocompatible [ 65 , 66 ]. Fig.…”
Cardiovascular disease (CVD) is a general term for conditions which are the leading cause of death in the world. Quick restoration of tissue perfusion is a key factor to combat these diseases and improve the quality and duration of patients' life. Revascularization techniques include angioplasty, placement of a stent, or surgical bypass grafting. For the latter technique, autologous vessels remain the best clinical option; however, many patients lack suitable autogenous due to previous operations and they are often unsuitable. Therefore, synthetic vascular grafts providing antithrombosis, neointimal hyperplasia inhibition and fast endothelialization are still needed. To address these limitations, 3D printed dipyridamole (DIP) loaded biodegradable vascular grafts were developed. Polycaprolactone (PCL) and DIP were successfully mixed without solvents and then vascular grafts were 3D printed. A mixture of high and low molecular weight PCL was used to better ensure the integration of DIP, which would offer the biological functions required above. Moreover, 3D printing technology provides the ability to fabricate structures of precise geometries from a 3D model, enabling to customize the vascular grafts' shape or size. The produced vascular grafts were fully characterized through multiple techniques and the last step was to evaluate their drug release, antiplatelet effect and cytocompatibility. The results suggested that DIP was properly mixed and integrated within the PCL matrix. Moreover, these materials can provide a sustained and linear drug release without any obvious burst release, or any faster initial release rates for 30 days. Compared to PCL alone, a clear reduced platelet deposition in all the DIP-loaded vascular grafts was evidenced. The hemolysis percentage of both materials PCL alone and PCL containing 20% DIP were lower than 4%. Moreover, PCL and 20% DIP loaded grafts were able to provide a supportive environment for cellular attachment, viability, and growth.
“…Эти результаты согласуются с данными других исследований гемосовместимости наногидроксиапатитов, полученных различными методами. Например, в [15] гидротермальным методом были получены образцы нанопористого гидроксиапатита, гемосовместимость которого определяли с помощью тестов на гемолиз, адгезию тромбоцитов, активацию тромбоцитов и измерения времени свертывания крови. Уровень гемолиза был менее 5%, что свидетельствует о высокой гемосовместимости образцов.…”
Section: результаты и их обсуждениеunclassified
“…В качестве контроля использова- ли интактную суспензию МЛ. Количественную оценку проводили с использованием МТТколориметрического теста [13][14][15]. Оценку индуцированного гемолиза проводили с использованием колориметрии, оценивая выход гемоглобина во внеклеточную среду в результате лизиса эритроцитов после контакта с испытуемыми образцами.…”
unclassified
“…Образцы оценивали как гемосовместимые при значении индуцированного гемолиза, не превышающем 2 ± 0.3%, с учетом существующих рекомендаций [10], а также результатов сходных исследований [15].…”
Синтетический наноструктурированный гидроксиапатит перспективен для широкого спектра задач восстановительной медицины и имплантологии, а также в косметологии и стоматологии. Меняя условия синтеза, для каждого конкретного направления применения можно регулировать характеристики материала в соответствии с поставленной задачей. Методом химического осаждения с последующей распылительной сушкой с ультразвуковой обработкой получены образцы наноструктурированного гидроксиапатита с различным составом и размером гранул. Анализ гемосовместимости материалов в экспериментах с мононуклеарными лейкоцитами и эритроцитами крови мыши показал, что полученные образцы гранул в концентрации 40 мг на 2 мл клеточной взвеси являются гемосовместимыми по всем исследуемым показателям независимо от размера и состава сфер. ВВЕДЕНИЕ В последние десятилетия во всем мире ведутся поиски биосовместимых материалов с целью использования их в травматологии, ортопедии и стоматологии. В клиническую практику внедрены импланты из керамики, биополимеров, металлов, углеродсодержащих и композитных материалов. Искусственный материал, замещающий кость, по своим физико-химическим и биологическим свойствам должен приближаться к нормальной костной ткани, быть остеосовместимым, способствовать оптимальному течению репаративных процессов.К наиболее распространенным биосовместимым материалам с ярко выраженными показателями биоактивности относятся искусственный и натуральный гидроксиапатит. В настоящее вре-мя для замещения костных дефектов в хирургической стоматологии, ортопедии и травматологии используется много различных форм гидроксиапатита, различающихся по форме и величине частиц. Считается, что синтетический гидроксиапатит по химическому составу и кристаллографическим показателям практически идентичен гидроксиапатиту нативной кости [1][2][3].Актуальной задачей при получении новых материалов на основе наногидроксиапатита является обеспечение возможности контролировать соотношения гидроксиапатита и фосфатов кальция, что позволит регулировать растворимость перспективного импланта от быстрорастворимого до нерастворимого (тетрафосфат кальция > α-трикальций фосфат > дигидрат дикальций фосфата > безводный дикальций фосфат ~ октокальций фосфат >
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