Physicochemical Composition and Antioxidant Activities of Korean Dioscorea Species

김경미,; 강문경,; 김진숙,; 김기창,; 최송이,

doi:10.17495/easdl.2015.10.25.5.880

Cited by 12 publications

(7 citation statements)

References 17 publications

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“…Saponins show potential as anticancer agents. As previously reported, wild species of Dioscorea families, including DN, were found to contain at least 10 to 20 times more dioscin, a major pharmacological component and a saponin exhibiting strong anticancer activity, than cultivated species, such as Dioscorea opposita or Dioscorea batatas [11]. This finding shows that DN is a very attractive material for commercialization, which can be realized through further research.…”

Section: Introductionsupporting

confidence: 67%

“…Therefore, the photodiode array (PDA) spectrum (190–800 nm) was used to determine the maximum absorbance of each compound, and a wavelength of 200 nm was chosen as the optimum wavelength for analysis. Reviews of UV/VIS analyses of several previously reported protodioscins have shown that the maximum absorbance of the compound is approximately 1.6 times greater at 200 nm [11], although a number of studies primarily selected wavelengths of 205 nm [8,24,25], possibly to reduce the effect of baseline signals from solvents or impurities. However, using a wavelength of 200 nm, the limit of detection (LOD) and limit of quantitation (LOQ) were determined by analyzing standards at various concentrations to evaluate whether this wavelength is suitable for developing an analysis method based on UV detection.…”

Section: Resultsmentioning

confidence: 99%

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Development of an Oriental Medicine Discrimination Method through Analysis of Steroidal Saponins in Dioscorea nipponica Makino and Their Anti-Osteosarcoma Effects

et al. 2019

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To prevent confusing Dioscorea nipponica (DN), an Oriental medicine, with Dioscorea quinquelobata (DQ) and Dioscorea septemloba (DS), a simple and accurate quantitative analysis method using HPLC combined with ultraviolet (UV) detection was developed and verified with UPLC-QTOF/MS through identification of five saponin glycosides: protodioscin (1), protogracillin (2), pseudoprotodioscin (3), dioscin (4), and gracillin (5). The newly developed analysis method showed sufficient reproducibility (<1.91%) and accuracy (92.1%–102.6%) and was able to identify DN based on the presence of compound 3 (13.821 ± 0.037 mg/mL) and the absence of 5. Compound 1, which is present in DN at a relatively high level (159.983 ± 0.064 mg/mL), was also an important marker for identification. Among the three species, DN showed the strongest activation of apoptotic signaling in osteosarcoma cells, while the four compounds detected in DN showed IC50 values of 6.43 (1), 10.61 (2), 10.48 (3), and 6.90 (4). In conclusion, the strong inhibitory effect of DN against osteosarcoma was confirmed to be associated with 1 and 4, which is also related to the quantitative results. Therefore, the results of this study might provide important information for quality control related to Oriental medicine.

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Section: Introductionsupporting

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Development of an Oriental Medicine Discrimination Method through Analysis of Steroidal Saponins in Dioscorea nipponica Makino and Their Anti-Osteosarcoma Effects

et al. 2019

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“…This result revealed that the antioxidant activity of DAN and DUN leaves was due to distinct metabolites. Kyung-Mi et al [ 35 ] reported that DAN roots showed a higher antioxidant activity than DUN roots, but the major metabolites contributing to antioxidant activity were not identified. Therefore, we examined discriminant metabolites present in DAN and DUN leaves collected in August, and found that they were positively correlated with the antioxidant activity and total phenolic contents, allowing us to postulate putative antioxidant compounds.…”

Section: Discussionmentioning

confidence: 99%

Metabolite Profiling of Dioscorea (Yam) Leaves to Identify Bioactive Compounds Reveals Their Potential as Renewable Resources

Kim

Son

Jeon

et al. 2021

Plants

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Yams (Dioscorea spp.) are cultivated and consumed as edible tubers, while their leaves are discarded as waste or burned with negative environmental impact. Herein, the metabolites of two yam species (Danma, DAN; Dunggeunma, DUN), harvested in June, July, and August, were profiled using GC-TOF-MS and UHPLC-LTQ-Orbitrap-MS/MS and the antioxidant activity of the extracts was evaluated to stimulate the utilization of yam leaves as a by-product. We observed that the relative levels of amino acids, organic acids, sugars, and saponins decreased linearly with prolonged harvest time, while fatty acid, phenanthrene, and flavonoid levels gradually increased. Furthermore, the leaf extracts obtained in August exhibited the highest antioxidant activity. To determine the antioxidant-contributing metabolites, OPLS-DA was performed for the leaf metabolites of DAN and DUN leaves harvested in August. Hydroxytyrosol-glucoside, apigenin-rhamnoside, and rutin were more abundant in DUN, while luteolin, phenanthrene derivatives, epicatechin, and kaempferide were relatively higher in DAN and their respective metabolites were positively correlated with the antioxidant activity. Moreover, secondary metabolites were more abundant in the leaves than in the roots, and consequently, the antioxidant activity of the former was also higher. Overall, the potential value of yam leaves as a renewable source of bioactive compounds is proposed.

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“…To evaluate the content of phenanthrenes in the Dioscorea genus, the subsequent quantitative analysis with HPLC-PDA was investigated on the peel extracts of four Dioscorea species, including D. batatas , D. polystachya , D. quinqueloba , and D. bulbifera . Notably, all investigated Dioscorea species were found to possess antioxidant effects in a previous report [ 32 ]. In the present study, the results showed that phenanthrene 1 was the most abundant in the peel of D. quinqueloba with content of 173.69 μg/g on the basis of weight.…”

Section: Resultsmentioning

confidence: 94%

Development and Validation of Quantitative Analysis Method for Phenanthrenes in Peels of the Dioscorea Genus

Kim

Cao

Yeo

et al. 2022

J. Microbiol. Biotechnol.

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Phenanthrenes are bioactive phenolic compounds found in genus Dioscorea, in which they are distributed more in peel than in flesh. Recent studies on phenanthrenes from Dioscorea sp. peels have revealed the potential for valuable biomaterials. Herein, an analytical method using highperformance liquid chromatography (HPLC) for quantitation of bioactive phenanthrenes was developed and validated. The calibration curves were obtained using the phenanthrenes (1-3) previously isolated from Dioscorea batatas concentrations in the range of 0.625-20.00 μg/ml with a satisfactory coefficient of determination (R 2 ) of 0.999. The limit of detection (LOD) and the limit of quantification (LOQ) values of the isolated phenanthrenes ranged from 0.78-0.89 and 2.38-2.71 μg/ml, respectively. The intraday and interday precision ranged from 0.25-7.58%. The recoveries of the isolated phenanthrenes were from 95 to 100% at concentrations of 1.25, 2.5, and 5.0 μg/ml. Additionally, phenanthrenes (1-3) were found in all investigated peel extracts. Hence, the developed method was encouraging for the quantitative analysis of phenanthrenes in genus Dioscorea.

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Physicochemical Composition and Antioxidant Activities of Korean Dioscorea Species

Cited by 12 publications

References 17 publications

Development of an Oriental Medicine Discrimination Method through Analysis of Steroidal Saponins in Dioscorea nipponica Makino and Their Anti-Osteosarcoma Effects

Development of an Oriental Medicine Discrimination Method through Analysis of Steroidal Saponins in Dioscorea nipponica Makino and Their Anti-Osteosarcoma Effects

Metabolite Profiling of Dioscorea (Yam) Leaves to Identify Bioactive Compounds Reveals Their Potential as Renewable Resources

Development and Validation of Quantitative Analysis Method for Phenanthrenes in Peels of the Dioscorea Genus

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