Physicochemical characterization of the staphylolytic LysK enzyme in complexes with polycationic polymers as a potent antimicrobial

Filatova, L. Yu.; Donovan, David M.; Becker, Stephen C.; Lebedev, Dmitry; Priyma, A.D.; Koudriachova, Helena V.; Kabanov, Alexander V.; Klyachko, Natalia L.

doi:10.1016/j.biochi.2013.04.013

Cited by 23 publications

(15 citation statements)

References 21 publications

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“…The stabilizing effect was attributed to enhanced structural ordering and breaking of aggregates due to electrostatic interactions. In addition, the lytic activity of LysK increased 3-to 3.5-fold when complexed with cationic polymers compared to the free form (193).…”

Section: Nanotechnology As a New Strategy For Endolysin Deliverymentioning

confidence: 97%

“…These stimuli may include temperature or pH changes; consequences of a host immune response, such as increased cytokine concentrations (185,186); and molecules released from infecting bacteria, such as enzymes, secreted toxins, and signaling molecules (187)(188)(189). Various polymers have been studied as potential drug release vehicles for research and clinical purposes, including poly(N-isopropylacrylamide) (PNIPAM) (190), chitosan (CS) (191,192), fucoidan (F) (192), PLLs (193), and poly(lactic-co-glycolic acid) (PLGA) (194). PNIPAM is a thermostatic polymer that is able to reversibly undergo a phase transition at its lower critical solution temperature (LCST), which leads to water expulsion, followed by a polymer volume change.…”

Section: Nanotechnology As a New Strategy For Endolysin Deliverymentioning

confidence: 99%

“…The generation of complexes consisting of endolysins and cationic polymers (PLLs) has also been reported as a possible way to produce antimicrobial agents with high activity against the target bacteria and enhanced stability at both storage and physiological temperatures. The stability of LysK in complexes with PLLs and their block copolymers with polyethylene glycol (PEG) was improved by both hydrophobic and electrostatic mechanisms (193). The stabilizing effect was attributed to enhanced structural ordering and breaking of aggregates due to electrostatic interactions.…”

Section: Nanotechnology As a New Strategy For Endolysin Deliverymentioning

confidence: 99%

See 2 more Smart Citations

Recombinant Endolysins as Potential Therapeutics against Antibiotic-Resistant Staphylococcus aureus: Current Status of Research and Novel Delivery Strategies

et al. 2018

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is one of the most common pathogens of humans and animals, where it frequently colonizes skin and mucosal membranes. It is of major clinical importance as a nosocomial pathogen and causative agent of a wide array of diseases. Multidrug-resistant strains have become increasingly prevalent and represent a leading cause of morbidity and mortality. For this reason, novel strategies to combat multidrug-resistant pathogens are urgently needed. Bacteriophage-derived enzymes, so-called endolysins, and other peptidoglycan hydrolases with the ability to disrupt cell walls represent possible alternatives to conventional antibiotics. These lytic enzymes confer a high degree of host specificity and could potentially replace or be utilized in combination with antibiotics, with the aim to specifically treat infections caused by Gram-positive drug-resistant bacterial pathogens such as methicillin-resistant . LysK is one of the best-characterized endolysins with activity against multiple staphylococcal species. Various approaches to further enhance the antibacterial efficacy and applicability of endolysins have been demonstrated. These approaches include the construction of recombinant endolysin derivatives and the development of novel delivery strategies for various applications, such as the production of endolysins in lactic acid bacteria and their conjugation to nanoparticles. These novel strategies are a major focus of this review.

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Section: Nanotechnology As a New Strategy For Endolysin Deliverymentioning

confidence: 97%

Section: Nanotechnology As a New Strategy For Endolysin Deliverymentioning

confidence: 99%

Section: Nanotechnology As a New Strategy For Endolysin Deliverymentioning

confidence: 99%

See 1 more Smart Citation

Recombinant Endolysins as Potential Therapeutics against Antibiotic-Resistant Staphylococcus aureus: Current Status of Research and Novel Delivery Strategies

et al. 2018

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“…The use of naturally derived biomacromolecules can further facilitate the biocompatibility of the resulting coacervate‐based materials, although a wide range of safe and highly effective synthetic polymers have also been demonstrated . Alternatively, materials with innate antimicrobial and/or antibacterial activities such as poly( l ‐lysine) or poly(ethyleneimine) can be taken advantage in some applications to circumvent the need for additional active ingredients while avoiding the potential for developing antibiotic resistant bacteria . Moving beyond the mere use of biological materials, researchers have also drawn inspiration from biological processes such as DNA–histone binding, heparin–growth factor interactions, and intracellular protein–RNA granules .…”

Section: Introductionmentioning

confidence: 99%

Complex coacervate‐based materials for biomedicine

Blocher

Perry

2016

WIREs Nanomed Nanobiotechnol

223

277

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There has been increasing interest in complex coacervates for deriving and transporting biomaterials. Complex coacervates are a dense, polyelectrolyte-rich liquid that results from the electrostatic complexation of oppositely-charged macro-ions. Coacervates have long been used as a strategy for encapsulation, particularly in food and personal care products. More recent efforts have focused on the utility of this class of materials for the encapsulation of small molecules, proteins, RNA, DNA, and other biomaterials for applications ranging from sensing to biomedicine. Furthermore, coacervate-related materials have found utility as in other areas of biomedicine, including cartilage mimics, tissue culture scaffolds, and adhesives for wet, biological environments. Here, we discuss the self-assembly of complex coacervate-based materials, current challenges in the intelligent design of these materials, and their utility applications in the broad field of biomedicine.

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“…Other combinations have also be tried to develop more selective and/or more potent agents against antibiotics-resistant pathogens. One example was conjugating AMPs to antibiotics like vancomycin using click chemistry [98,99,100]. Another example was fusing the binding domain of FyuA, which is an outer membrane transporter protein of Yersinia pestis , to the N-terminus of T4 lysozyme similar to pesticin [101,102].…”

Section: Future Directions and Concluding Remarksmentioning

confidence: 99%

Anti-Mycobacterial Peptides: From Human to Phage

Teng

Liu

Wei

2015

Cell Physiol Biochem

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Mycobacterium tuberculosis is the major pathogen of tuberculosis (TB). With the growing problem of M. tuberculosis resistant to conventional antibiotics, especially multi-drug resistant tuberculosis (MDR-TB) and extensively-drug resistant tuberculosis (XDR-TB), the need for new TB drugs is now more prominent than ever. Among the promising candidates for anti-TB drugs, anti-mycobacterial peptides have a few advantages, such as low immunogenicity, selective affinity to prokaryotic negatively charged cell envelopes, and diverse modes of action. In this review, we summarize the recent progress in the anti-mycobacterial peptides, highlighting the sources, effectiveness and bactericidal mechanisms of these antimicrobial peptides. Most of the current anti-mycobacterial peptides are derived either from host immune cells, bacterial extraction, or mycobacteriophages. Besides trans-membrane pore formation, which is considered to be the common bactericidal mechanism, many of the anti-mycobacterial peptides have the second non-membrane targets within mycobacteria. Additionally, some antimicrobial peptides play critical roles in innate immunity. However, a few obstacles, such as short half-life in vivo and resistance to antimicrobial peptides, need overcoming before clinical applications. Nevertheless, the multiple functions of anti-mycobacterial peptides, especially direct killing of pathogens and immune-modulators in infectious and inflammatory conditions, indicate that they are promising candidates for future drug development.

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Physicochemical characterization of the staphylolytic LysK enzyme in complexes with polycationic polymers as a potent antimicrobial

Cited by 23 publications

References 21 publications

Recombinant Endolysins as Potential Therapeutics against Antibiotic-Resistant Staphylococcus aureus: Current Status of Research and Novel Delivery Strategies

Recombinant Endolysins as Potential Therapeutics against Antibiotic-Resistant Staphylococcus aureus: Current Status of Research and Novel Delivery Strategies

Complex coacervate‐based materials for biomedicine

Anti-Mycobacterial Peptides: From Human to Phage

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