Physicochemical characterization of pH-responsive and fusogenic self-assembled non-phospholipid vesicles for a potential multiple targeting therapy

Francesco, Martina Di; Celia, Christian; Primavera, Rosita; d’Avanzo, Nicola; Locatelli, Marcello; Fresta, Massimo; Cilurzo, Felisa; Ventura, Cinzia Anna; Paolino, Donatella; Marzio, Luisa Di

doi:10.1016/j.ijpharm.2017.05.055

Cited by 24 publications

(44 citation statements)

References 86 publications

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“…Liposomes (LIPs) were prepared by thin-layer evaporation (TLE). 51 Briefly, DPPC, cholesterol, DSPE-PEG, and DSPE-MTX were dissolved in chloroform in a round-bottomed flask (ratio 6:3:1:1). The thin-layer lipid film was obtained with the evaporation of the organic solvent at 60° under reduced pressure.…”

Section: Materials and Methodsmentioning

confidence: 99%

Modulating Lipoprotein Transcellular Transport and Atherosclerotic Plaque Formation in ApoE^–/– Mice via Nanoformulated Lipid–Methotrexate Conjugates

Francesco

Gurgone

Palomba

et al. 2020

ACS Appl. Mater. Interfaces

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Macrophage inflammation and maturation into foam cells, following the engulfment of oxidized low-density lipoproteins (oxLDL), are major hallmarks in the onset and progression of atherosclerosis. Yet, chronic treatments with anti-inflammatory agents, such as methotrexate (MTX), failed to modulate disease progression, possibly for the limited drug bioavailability and plaque deposition. Here, MTX–lipid conjugates, based on 1,2-distearoyl- sn -glycero-3-phosphoethanolamine (DSPE), were integrated in the structure of spherical polymeric nanoparticles (MTX-SPNs) or intercalated in the lipid bilayer of liposomes (MTX-LIP). Although, both nanoparticles were colloidally stable with an average diameter of ∼200 nm, MTX-LIP exhibited a higher encapsulation efficiency (>70%) and slower release rate (∼50% at 10 h) compared to MTX-SPN. In primary bone marrow derived macrophages (BMDMs), MTX-LIP modulated the transcellular transport of oxLDL more efficiently than free MTX mostly by inducing a 2-fold overexpression of ABCA1 (regulating oxLDL efflux), while the effect on CD36 and SRA-1 (regulating oxLDL influx) was minimal. Furthermore, in BMDMs, MTX-LIP showed a stronger anti-inflammatory activity than free MTX, reducing the expression of IL-1β by 3-fold, IL-6 by 2-fold, and also moderately of TNF-α. In 28 days high-fat-diet-fed apoE –/– mice, MTX-LIP reduced the mean plaque area by 2-fold and the hematic amounts of RANTES by half as compared to free MTX. These results would suggest that the nanoenhanced delivery to vascular plaques of the anti-inflammatory DSPE-MTX conjugate could effectively modulate the disease progression by halting monocytes’ maturation and recruitment already at the onset of atherosclerosis.

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Section: Materials and Methodsmentioning

confidence: 99%

Modulating Lipoprotein Transcellular Transport and Atherosclerotic Plaque Formation in ApoE^–/– Mice via Nanoformulated Lipid–Methotrexate Conjugates

Francesco

Gurgone

Palomba

et al. 2020

ACS Appl. Mater. Interfaces

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“…Another study, by Di Francesco et al . showed the advantages of using pH-sensitive nanoparticles as a fusogenic drug delivery system (Di Francesco et al, 2017 ). The nanoparticles were formulated according to their target cells, and physicochemical properties were measured by DLS and fluorescence spectroscopy.…”

Section: Nanoparticles In Therapeuticsmentioning

confidence: 99%

“…DLS was used here not only as a mere characterization technique, but as a tool to identify metal structures with higher colloidal stability and better size distribution (Shmarakov et al, 2017 ). Di Francesco et al ., using non-ionic surfactant vesicles (NSVs) loaded with DOX, developed nanosystems with different ratios of Tween21/Tween80, promoting a pH-responsive approach with anticancer properties (Di Francesco et al, 2017 ). These NSVs showed a fusogenic behavior and an increased targeting efficiency, which translated in higher anticancer activity.…”

Section: Nanoparticles In Therapeuticsmentioning

confidence: 99%

Application of Light Scattering Techniques to Nanoparticle Characterization and Development

et al. 2018

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Over the years, the scientific importance of nanoparticles for biomedical applications has increased. The high stability and biocompatibility, together with the low toxicity of the nanoparticles developed lead to their use as targeted drug delivery systems, bioimaging systems, and biosensors. The wide range of nanoparticles size, from 10 nm to 1 μm, as well as their optical properties, allow them to be studied using microscopy and spectroscopy techniques. In order to be effectively used, the physicochemical properties of nanoparticle formulations need to be taken into account, namely, particle size, surface charge distribution, surface derivatization and/or loading capacity, and related interactions. These properties need to be optimized considering the final nanoparticle intended biodistribution and target. In this review, we cover light scattering based techniques, namely dynamic light scattering and zeta-potential, used for the physicochemical characterization of nanoparticles. Dynamic light scattering is used to measure nanoparticles size, but also to evaluate their stability over time in suspension, at different pH and temperature conditions. Zeta-potential is used to characterize nanoparticles surface charge, obtaining information about their stability and surface interaction with other molecules. In this review, we focus on nanoparticle characterization and application in infection, cancer and cardiovascular diseases.

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“…Furthermore, the nanocarriers can effectively encapsulate various types of small molecules with different physicochemical features including hydrophilicity (23), hydrophobicity (24), electrical charge (25), and size (26). Enhanced permeability and a retention (EPR) effect has also been reported and serves as a key rationale for using nanocarriers to treat solid tumors (27–29). However, the targeted and uniform delivery of the nanocarriers to tumors in sufficient quantities requires optimization.…”

Section: Introductionmentioning

confidence: 99%

Folic Acid Receptor-Mediated Targeting Enhances the Cytotoxicity, Efficacy, and Selectivity of Withania somnifera Leaf Extract: In vitro and in vivo Evidence

Wang

Kaul³

et al. 2019

Front. Oncol.

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Nanomedicine holds great potential for drug delivery to achieve more effective and safer cancer treatment. Earlier, we reported that the alcoholic extract of Withania somnifera leaves (i-Extract) has selective cancer cell killing activity. Herein, we developed a folate receptor-targeting i-Extract nanocomplex (FRi-ExNC) that suspends well in water and possesses enhanced selective anticancer activity in both in vitro and in vivo assays. Comparative analyses of folate receptor (FR)-positive and -negative cells revealed that FRi-ExNC caused a stronger decrease in Cyclin D/Cdk4 and anti-apoptotic protein Bcl-2, as well as a higher increase in the growth arrest regulating protein p21 WAF1 and pro-apoptotic protein PARP-1, in FR-enriched cancer cells. Our results demonstrate that FRi-ExNC could be a natural source-based nanomedicine for targeted cancer therapy.

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Physicochemical characterization of pH-responsive and fusogenic self-assembled non-phospholipid vesicles for a potential multiple targeting therapy

Cited by 24 publications

References 86 publications

Modulating Lipoprotein Transcellular Transport and Atherosclerotic Plaque Formation in ApoE^–/– Mice via Nanoformulated Lipid–Methotrexate Conjugates

Modulating Lipoprotein Transcellular Transport and Atherosclerotic Plaque Formation in ApoE^–/– Mice via Nanoformulated Lipid–Methotrexate Conjugates

Application of Light Scattering Techniques to Nanoparticle Characterization and Development

Folic Acid Receptor-Mediated Targeting Enhances the Cytotoxicity, Efficacy, and Selectivity of Withania somnifera Leaf Extract: In vitro and in vivo Evidence

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Physicochemical characterization of pH-responsive and fusogenic self-assembled non-phospholipid vesicles for a potential multiple targeting therapy

Cited by 24 publications

References 86 publications

Modulating Lipoprotein Transcellular Transport and Atherosclerotic Plaque Formation in ApoE–/– Mice via Nanoformulated Lipid–Methotrexate Conjugates

Modulating Lipoprotein Transcellular Transport and Atherosclerotic Plaque Formation in ApoE–/– Mice via Nanoformulated Lipid–Methotrexate Conjugates

Application of Light Scattering Techniques to Nanoparticle Characterization and Development

Folic Acid Receptor-Mediated Targeting Enhances the Cytotoxicity, Efficacy, and Selectivity of Withania somnifera Leaf Extract: In vitro and in vivo Evidence

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Product

Resources

About

Modulating Lipoprotein Transcellular Transport and Atherosclerotic Plaque Formation in ApoE^–/– Mice via Nanoformulated Lipid–Methotrexate Conjugates

Modulating Lipoprotein Transcellular Transport and Atherosclerotic Plaque Formation in ApoE^–/– Mice via Nanoformulated Lipid–Methotrexate Conjugates