Physicochemical Characterization of Fucoidans from Sargassum henslowianum C.Agardh and Their Antithrombotic Activity In Vitro

Lin, Pei‐Chun; Chen, Suhua; Lü, Min; Wang, Weimin

doi:10.3390/md20050300

Cited by 9 publications

(4 citation statements)

References 43 publications

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“…In comparison to the fractions F1 and DF1 (1250 and 1254 cm −1 ), the absorption peak of S=O of F2 and DF2 (1248 and 1243 cm −1 ) was significantly enhanced, consistent with the somewhat higher sulfate group contents ( Table 1 ). The absorption peak at 1033–1049 cm −1 is attributed to the stretching vibration of C-O-C and the angular vibration of C-O in the sugar ring C-O-C, while the characteristic absorption peak at about 900 cm −1 is caused by β-glycosidic bonds [ 24 ]. The absorption at 812–846 cm −1 indicates the mode of sulfation.…”

Section: Resultsmentioning

confidence: 99%

Physicochemical Characterization and Antitumor Activity of Fucoidan and Its Degraded Products from Sargassum hemiphyllum (Turner) C. Agardh

et al. 2023

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Fucoidan has many biological functions, including anti-tumor activity. Additionally, it has been suggested that low-molecular-weight fucoidans have greater bioactivities. This study aimed to examine the degradation, purification, physicochemical characterization and in vitro antitumor activity of fucoidan from Sargassum hemiphyllum (Turner) C. Agardh. Fucoidan was isolated using DEAE-cellulose-52 (F1, F2), Vc-H2O2 degration, and Sepharose CL-6B gel (DF1, DF2) from crude Sargassum fucoidans. Physicochemical characteristics of four isolated fucoidans were examined using chemical and monosaccharide composition, average molecular weight (Mw), and FTIR. Furthermore, the anti-proliferative effects of purified fucoidans on human hepatocellular carcinoma cells (HepG2), human Burkitt Lymphoma cells (MCF-7), human uterine carcinoma cells (Hela) and human lung cancer cells (A549) were analyzed by MTT method. The apoptosis of HepG2 cells was detected by flow cytometry. Our data suggest that the contents of polysaccharide, L-fucose and sulfate of DF2 were the highest, which were 73.93%, 23.02% and 29.88%, respectively. DF1 has the smallest molecular weight (14,893 Da) followed by DF2 (21,292 Da). The four fractions are mainly composed of fucose, mannose and rhamnose, and the infrared spectra are similar, all of which contain polysaccharide and sulfate characteristic absorption peaks. The results of MTT assay showed that the four fractions had inhibitory effects on HepG2 and A549 in the range of 0.5–8 mg/mL, and the four fractions had strong cytotoxic effects on HepG2 cells. DF2 had the best inhibitory effect on HepG2 (IC50 = 2.2 mg/mL). In general, the antitumor activity of Sargassum fucoidans is related to the content of L-fucose, sulfate and molecular weight, and Sargassum fucoidan has the best inhibitory effect on HepG2 hepatocellular carcinoma cells. Furthermore, when compared to MCF-7, Hela, and A549 cells, Sargassum fucoidans had the best capacity to reduce the viability of human hepatocellular carcinoma cells (HepG2) and to induce cell apoptosis, proving itself to have a good potential in anti-liver cancer therapy.

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Section: Resultsmentioning

confidence: 99%

Physicochemical Characterization and Antitumor Activity of Fucoidan and Its Degraded Products from Sargassum hemiphyllum (Turner) C. Agardh

et al. 2023

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“…According to our previous method, crude fucoidans from Sargassum spp. were extracted [ 19 ]. According to the ratio of material to liquid at 1:30, dried sargassum was added to distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…According to Wang et al [ 32 ] and our previous method [ 19 ], size-exclusion chromatography was used to detect Mw. In short, fucoidan (ZF1, ZF2, and ZF3) and 0.5 mol/L NaCl were mixed into 10 mg/mL solution and filtered through 0.22 μm membrane and Sephacryl Smur300 HR gel column.…”

Section: Methodsmentioning

confidence: 99%

Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans

Lin

Chen

Zhong

2022

Foods

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Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities. In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified. The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated. The results showed that the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF) was as high as13.63%. Its ability of binding bile acid was better than other five crude fucoidans. Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals. The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively. Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose. Three fucoidans can bind to bile acid, and ZF2 showed the best binding capability. In vitro experiments showed that ZF1, ZF2, and ZF3 could reduce intracellular total cholesterol (TC) content in HepG2 cells without affecting their viability. ZF2 showed the best ability to reduce TC.

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“…Sargassumfucoidan is a kind of sulfatedheteropolysaccharide with a variety of biological activities. The aim of this study was to investigate the extraction, purification, physicochemical characterization and in vitro antithrombotic activity of fucoidan from Sargassumhenslowianum (Lin et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Pharmacological potential of Sargassum sp. of west coast of Maharashtra Kunkeshwar, India

Abdi

Karande²,

Mohammed

et al. 2022

Front. Mar. Sci.

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A promising plant group that is important in medicine is the seaweed. In this investigation, we have focused on the pharmacological prospects of brown algae (Sargassum sp). Nutraceuticals refer to products that may combine pharmacological and nutrition. We have collected the plant material close to Kunkeeshwar in Sindhudurg, Maharashtra’s western coast. We focused on the Physicochemical Attributes of Sargassum sp., and the study found that the ash value (41 ± 0.67), moisture content (1.33 ± 0.038), alcohol-soluble extractive value (1.5 ± 0.030), moisture content (1.33 ± 0.038), and total cash value (41 ± 0.67) are all stated in (%).The GCMS findings, which demonstrate that a total of 25 chemicals were discovered in the aqueous extract of Sargassum sp., also represent their nutritional profile. All of these substances have chromatograms that can be observed.1-Hexyl-2-nitrocyclohexane is the substance with the second-largest retention duration and relative areas, followed by n-hexadanoic acid. The presence of ocladecanoic acid was later determined. The explanation for how the activity is concentration-dependent is pharmacological activity, particularly antioxidant activity as shown by the DPPH assay.2μg/mL is -1545.4 ± 0.05. as a minimum. and at (32 g/mL is 7.67 ± 0.05) is the highest. The antifungal activity of Candida albicans (12.8 mm) and Aspergillus niger (14.4 mm) both exhibit zone inhibition when tested against two distinct pathogen however, the antibacterial activity of three different bacteria demonstrates that Staphylococcus aureus (12 mm), Bacillus subtilis (10 mm), Proteus mirabilis (12.8 mm), and Escherichia. coli are all zone inhibited (13mm). Brown algae offer the greatest potential for human health nutrition, while pharmacological analysis is helpful for therapeutic application, according to the overall analysis.

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Physicochemical Characterization of Fucoidans from Sargassum henslowianum C.Agardh and Their Antithrombotic Activity In Vitro

Cited by 9 publications

References 43 publications

Physicochemical Characterization and Antitumor Activity of Fucoidan and Its Degraded Products from Sargassum hemiphyllum (Turner) C. Agardh

Physicochemical Characterization and Antitumor Activity of Fucoidan and Its Degraded Products from Sargassum hemiphyllum (Turner) C. Agardh

Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans

Pharmacological potential of Sargassum sp. of west coast of Maharashtra Kunkeshwar, India

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