Physicochemical characterization of drug nanocarriers

Manaia, Eloísa Berbel; Abuçafy, Marina Paiva; Chiari-Andréo, Bruna Galdorfini; Silva, Bruna Lallo; Oshiro, João Augusto

doi:10.2147/ijn.s133832

Cited by 139 publications

(44 citation statements)

References 103 publications

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“…Based on diameter, small (<50 nm), large (50-500 nm) and giant (>500 nm) liposomes can be distinguished (Banerjee, 2001;Morton et al, 2012). Alternatively, a classification can be made on the basis of whether a liposome possesses uni-, oligo-, or multi-lamellar bilayers (Morton et al, 2012;Manaia et al, 2017). Liposomes can consist of naturallyoccurring lipids or synthetically-made lipids (sometimes called "artificial" liposomes).…”

Section: Summary Of Different Types Of Liposomesmentioning

confidence: 99%

“…Within the range of DOPA concentrations applied, zeta potentials remained below the critical limit of FIGURE 4 | Zeta potentials of liposomes. Liposome suspensions are considered to be unstable when their zeta potential is between −30 and +30 mV (Manaia et al, 2017). Zeta potentials between −10 and +10 mV are considered to represent uncharged liposomes.…”

Section: Cationic Liposomesmentioning

confidence: 99%

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Lipid-Based Antimicrobial Delivery-Systems for the Treatment of Bacterial Infections

et al. 2020

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Many nanotechnology-based antimicrobials and antimicrobial-delivery-systems have been developed over the past decades with the aim to provide alternatives to antibiotic treatment of infectious-biofilms across the human body. Antimicrobials can be loaded into nanocarriers to protect them against de-activation, and to reduce their toxicity and potential, harmful side-effects. Moreover, antimicrobial nanocarriers such as micelles, can be equipped with stealth and pH-responsive features that allow self-targeting and accumulation in infectious-biofilms at high concentrations. Micellar and liposomal nanocarriers differ in hydrophilicity of their outer-surface and inner-core. Micelles are self-assembled, spherical core-shell structures composed of single layers of surfactants, with hydrophilic head-groups and hydrophobic tail-groups pointing to the micellar core. Liposomes are composed of lipids, self-assembled into bilayers. The hydrophilic head of the lipids determines the surface properties of liposomes, while the hydrophobic tail, internal to the bilayer, determines the fluidity of liposomal-membranes. Therefore, whereas micelles can only be loaded with hydrophobic antimicrobials, hydrophilic antimicrobials can be encapsulated in the hydrophilic, aqueous core of liposomes and hydrophobic or amphiphilic antimicrobials can be inserted in the phospholipid bilayer. Nanotechnology-derived liposomes can be prepared with diameters <100-200 nm, required to prevent reticulo-endothelial rejection and allow penetration into infectious-biofilms. However, surface-functionalization of liposomes is considerably more difficult than of micelles, which explains while self-targeting, pH-responsive liposomes that find their way through the blood circulation toward infectious-biofilms are still challenging to prepare. Equally, development of liposomes that penetrate over the entire thickness of biofilms to provide deep killing of biofilm inhabitants still provides a challenge. The liposomal phospholipid bilayer easily fuses with bacterial cell membranes to release high antimicrobial-doses directly inside bacteria. Arguably, protection against de-activation of antibiotics in liposomal nanocarriers and their fusogenicity constitute the biggest advantage of liposomal antimicrobial carriers over antimicrobials free in solution. Many Gram-negative and Gram-positive bacterial strains, resistant to specific antibiotics, Wang et al. Lipid-Based Antimicrobial Delivery-Systems have been demonstrated to be susceptible to these antibiotics when encapsulated in liposomal nanocarriers. Recently, also progress has been made concerning large-scale production and long-term storage of liposomes. Therewith, the remaining challenges to develop self-targeting liposomes that penetrate, accumulate and kill deeply in infectious-biofilms remain worthwhile to pursue.

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Section: Summary Of Different Types Of Liposomesmentioning

confidence: 99%

Section: Cationic Liposomesmentioning

confidence: 99%

Lipid-Based Antimicrobial Delivery-Systems for the Treatment of Bacterial Infections

et al. 2020

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“…This behavior increases their solubility in the medium and decreases their surface-to-volume ratio. Liposomes can be prepared through reverse-phase evaporation, the injection of phospholipids dissolved in an organic phase into a drug-containing aqueous phase, detergent dialysis, microfluidic hydrodynamic focus, supercritical reverse-phase evaporation, electroformation, microfluid liposome formation, and thin lipid film formation [126].…”

Section: Polymersomes and Liposomesmentioning

confidence: 99%

Polymers and Polymer Nanocomposites for Cancer Therapy

Feldman

2019

Applied Sciences

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Synthetic polymers, biopolymers, and their nanocomposites are being studied, and some of them are already used in different medical areas. Among the synthetic ones that can be mentioned are polyolefins, fluorinated polymers, polyesters, silicones, and others. Biopolymers such as polysaccharides (chitosan, hyaluronic acid, starch, cellulose, alginates) and proteins (silk, fibroin) have also become widely used and investigated for applications in medicine. Besides synthetic polymers and biopolymers, their nanocomposites, which are hybrids formed by a macromolecular matrix and a nanofiller (mineral or organic), have attracted great attention in the last decades in medicine and in other fields due to their outstanding properties. This review covers studies done recently using the polymers, biopolymers, nanocomposites, polymer micelles, nanomicelles, polymer hydrogels, nanogels, polymersomes, and liposomes used in medicine as drugs or drug carriers for cancer therapy and underlines their responses to internal and external stimuli able to make them more active and efficient. They are able to replace conventional cancer drug carriers, with better results. Appl. Sci. 2019, 9, 3899 2 of 24 nanocomposites offer numerous opportunities in diverse applications, including nanocarriers, tissue engineering, antimicrobials, sensors, etc. These new groups of materials have gained significant research interest due to their novel properties, which are gained through the addition of nanofillers. Polymer nanocomposites have significant potential in disease theranostics, and nanotechnology brings great promise in cancer drug carriers [4].Chemotherapy implies the use of drugs and, besides the drugs, carriers. Common products used as carriers include synthetic polymers, biopolymers, and polymer nanocomposites.Some of the most useful drugs for chemotherapy are toxic chemicals able to inhibit the proliferation of cancer cells. The use of chemotherapeutic drugs has been in part hindered by their poor solubility in water, their short biological half-life, their lack of targeting ability, and the development of multidrug resistance [5]. In the last decades, nanoparticles have shown significant promise as an oncology treatment modality. Responsive polymers represent a promising class of nanoparticles that can trigger delivery through the exploitation of a specific stimuli. Response to a stimulus is one of the most basic processes found in living systems. A tutorial review highlighted the recent developments in polymer-based approaches to internally responsive nanoparticles for oncology [6].One important goal of medicine is to develop carriers that can selectively deliver anticancer drugs to tumor cells with no or minimal side effects in healthy cells [7,8].Polymers and their nanocomposites in different forms, such as micelles, hydrogels, polymersomes, and liposomes, have important potential in cancer diagnosis and treatment.Nanocomposites are popular in many areas due to properties such as their unique design capacity, eco-friendly nature, ...

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“…Representative graphs based on an independent measurement of the zeta potential and particle size distribution are shown in Figure 1a,b. Researchers have shown that TEM imaging is an effective and powerful tool for characterizing the morphology of nano-and micro-structured biomaterials and drug carriers as it uses more powerful beams to produce higher resolution images with more details and information [30,31]. The TEM micrographs showed minimally aggregated, dispersed RDS particles that appeared as darker areas relative to the background, with rounded outer morphologies (Figure 1c,d).…”

Section: Size Polydispersity Index and Zeta Potential Determinationmentioning

confidence: 99%

Development and Evaluation of a Reconstitutable Dry Suspension Containing Isoniazid for Flexible Pediatric Dosing

2020

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Tuberculosis (TB) is a major cause of childhood death. Despite the startling statistics, it is neglected globally as evidenced by treatment and clinical care schemes, mostly extrapolated from studies in adults. The objective of this study was to formulate and evaluate a reconstitutable dry suspension (RDS) containing isoniazid, a first-line anti-tubercular agent used in the treatment and prevention of TB infection in both children and adults. The RDS formulation was prepared by direct dispersion emulsification of an aqueous-lipid particulate interphase coupled with lyophilization and dry milling. The RDS appeared as a cream-white free-flowing powder with a semi-crystalline and microparticulate nature. Isoniazid release was characterized with an initial burst up to 5 minutes followed by a cumulative release of 67.88% ± 1.88% (pH 1.2), 60.18% ± 3.33% (pH 6.8), and 49.36% ± 2.83% (pH 7.4) over 2 h. An extended release at pH 7.4 and 100% drug liberation was achieved within 300 min. The generated release profile best fitted the zero order kinetics (R2 = 0.976). RDS was re-dispersible and remained stable in the dried and reconstituted states over 4 months and 11 days respectively, under common storage conditions.

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Physicochemical characterization of drug nanocarriers

Cited by 139 publications

References 103 publications

Lipid-Based Antimicrobial Delivery-Systems for the Treatment of Bacterial Infections

Lipid-Based Antimicrobial Delivery-Systems for the Treatment of Bacterial Infections

Polymers and Polymer Nanocomposites for Cancer Therapy

Development and Evaluation of a Reconstitutable Dry Suspension Containing Isoniazid for Flexible Pediatric Dosing

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