Physicochemical characterization of a PEGylated liposomal drug formulation using capillary electrophoresis

Franzen, Ulrik; Vermehren, Charlotte; Jensen, Henrik; Østergaard, Jesper

doi:10.1002/elps.201000552

Cited by 47 publications

(37 citation statements)

References 58 publications

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“…Therefore, the potential therapeutic application of a given liposome is highly influenced by its average size and, consequently, by the possible liposome-cell interactions at the target sites of action. Table 2 lists some techniques used to physically characterize the liposomes, in terms of the size distribution and zeta field-flow fractionation [68 -70] zeta potential LDE capillary electrophoresis [68,71] rsif.royalsocietypublishing.org J. R. Soc. Interface 11: 20140459…”

Section: Liposome Characterization Techniquesmentioning

confidence: 99%

Liposomes in tissue engineering and regenerative medicine

Monteiro

Martins

Reis

et al. 2014

J. R. Soc. Interface.

301

217

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Liposomes are vesicular structures made of lipids that are formed in aqueous solutions. Structurally, they resemble the lipid membrane of living cells. Therefore, they have been widely investigated, since the 1960s, as models to study the cell membrane, and as carriers for protection and/or delivery of bioactive agents. They have been used in different areas of research including vaccines, imaging, applications in cosmetics and tissue engineering. Tissue engineering is defined as a strategy for promoting the regeneration of tissues for the human body. This strategy may involve the coordinated application of defined cell types with structured biomaterial scaffolds to produce living structures. To create a new tissue, based on this strategy, a controlled stimulation of cultured cells is needed, through a systematic combination of bioactive agents and mechanical signals. In this review, we highlight the potential role of liposomes as a platform for the sustained and local delivery of bioactive agents for tissue engineering and regenerative medicine approaches.

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Section: Liposome Characterization Techniquesmentioning

confidence: 99%

Liposomes in tissue engineering and regenerative medicine

Monteiro

Martins

Reis

et al. 2014

J. R. Soc. Interface.

301

217

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“…The ease of use of DLS and its wide availability made it a popular technique for particle sizing, however the presence of large aggregates or dust, which scatter more than the small particles, tends to bias the measured average hydrodynamic radius toward larger size, mainly at low scattering angles (Cottet et al, 2007;Fischer and Schmidt, 2016;Franzen et al, 2011). Another widely used technique, particularly in biological applications, is the fluorescence correlation spectroscopy (FCS).…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic size characterization of a self-emulsifying lipid pharmaceutical excipient by Taylor dispersion analysis with fluorescent detection

Chamieh

Jannin²,

Demarne³

et al. 2016

International Journal of Pharmaceutics

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“…The increased viscosity decreases the mobility of the charged analytes. As a result, the mobility of Apep10 was corrected according to Franzen et al [26]. The electropherograms of Apep10 and acetone in the running buffer with various concentrations of the E .…”

Section: Resultsmentioning

confidence: 99%

Interaction between Antibacterial Peptide Apep10 and Escherichia coli Membrane Lipids Evaluated Using Liposome as Pseudo-Stationary Phase

Tang¹,

Pu²,

Li³

2017

PLoS ONE

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Liposomes constructed from Escherichia coli membrane lipids were used as a pseudo-stationary phase in capillary electrophoresis and immobilised liposome chromatography to evaluate the interaction between antibacterial peptide (ABP) Apep10 and bacterial membrane lipids. The peptide mobility decreased as the concentration of liposomes increased, providing evidence for the existence of this interaction. The binding constant between Apep10 and the Escherichia coli membranes lipid liposome was higher than that of Apep10 with a mixed phospholipids liposome at the same temperature. The capillary electrophoresis results indicate that the binding ability of Apep10 with a liposome was dependent on the liposome’s lipid compositions. Thermodynamic analysis by immobilised liposome chromatography indicated that hydrophobic and electrostatic effects contributed to the partitioning of Apep10 in the membrane lipids. The liposomes constructed from bacterial membrane lipid were more suitable as the model membranes used to study dynamic ABP/membrane interactions than those constructed from specific ratios of particular phospholipids, with its more biomimetic phospholipid composition and contents. This study provides an appropriate model for the evaluation of ABP-membrane interactions.

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Physicochemical characterization of a PEGylated liposomal drug formulation using capillary electrophoresis

Cited by 47 publications

References 58 publications

Liposomes in tissue engineering and regenerative medicine

Liposomes in tissue engineering and regenerative medicine

Hydrodynamic size characterization of a self-emulsifying lipid pharmaceutical excipient by Taylor dispersion analysis with fluorescent detection

Interaction between Antibacterial Peptide Apep10 and Escherichia coli Membrane Lipids Evaluated Using Liposome as Pseudo-Stationary Phase

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