Physicochemical characterization and oxidative potential of size fractionated Particulate Matter: Uptake, genotoxicity and mutagenicity in V-79 cells

Dubey, Kavita; Maurya, Ranjana; Mourya, Durgesh; Pandey, Alok Kumar

doi:10.1016/j.ecoenv.2022.114205

Cited by 6 publications

(2 citation statements)

References 55 publications

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“…The alkaline single-cell gel electrophoresis (SCGE) assay, commonly known as the comet assay, was employed to evaluate DNA strand breaks following a methodology adapted from Dubey et al 58 . HaCaT cells were cultured in a 6-well plate at a density of 2 × 10 5 cells/ml.…”

Section: Methodsmentioning

confidence: 99%

Sunset Yellow protects against oxidative damage and exhibits chemoprevention in chemically induced skin cancer model

Singh,

Yadav,

Cavallo

et al. 2024

npj Syst Biol Appl

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Skin cancer and other skin-related inflammatory pathologies are rising due to heightened exposure to environmental pollutants and carcinogens. In this context, natural products and repurposed compounds hold promise as novel therapeutic and preventive agents. Strengthening the skin’s antioxidant defense mechanisms is pivotal in neutralizing reactive oxygen species (ROS) and mitigating oxidative stress. Sunset Yellow (SY) exhibits immunomodulatory characteristics, evidenced by its capacity to partially inhibit the secretion of proinflammatory cytokines, regulate immune cell populations, and modulate the activation of lymphocytes. This study aimed to investigate the antioxidant and anti-genotoxic properties of SY using in-silico, in vitro, and physiochemical test systems, and to further explore its potential role in 7,12-dimethylbenz(a) anthracene (DMBA)/ 12-o-tetradecanoylphorbol-13-acetate (TPA)-induced two-stage skin carcinogenesis. In vitro experiments showed that pre-treatment of SY significantly enhanced the cell viability of HaCaT cells when exposed to tertiary-Butyl Hydrogen Peroxide (tBHP). This increase was accompanied by reduced ROS levels, restoration of mitochondrial membrane potential, and notable reduction in DNA damage in (SY + tBHP) treated cells. Mechanistic investigations using DPPH chemical antioxidant activity test and potentiometric titrations confirmed SY’s antioxidant properties, with a standard reduction potential ($${E}^{o}$$ E o ) of 0.211 V. Remarkably, evaluating the effect of topical application of SY in DMBA/TPA-induced two-step skin carcinogenesis model revealed dose-dependent decreases in tumor latency, incidence, yield, and burden over 21-weeks. Furthermore, computational analysis and experimental validations identified GSK3β, KEAP1 and EGFR as putative molecular targets of SY. Collectively, our findings reveal that SY enhances cellular antioxidant defenses, exhibits anti-genotoxic effects, and functions as a promising chemopreventive agent.

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Section: Methodsmentioning

confidence: 99%

Sunset Yellow protects against oxidative damage and exhibits chemoprevention in chemically induced skin cancer model

Singh,

Yadav,

Cavallo

et al. 2024

npj Syst Biol Appl

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Section: The Alkaline Single-cell Gel Electrophoresis Assay (Cometmentioning

confidence: 99%

Sunset Yellow protects against oxidative damage in human keratinocyte-derived HaCaT cells and exhibits chemopreventive efficacy in DMBA/TPA-induced skin carcinogenesis

Gupta

Singh

Yadav

et al. 2023

Preprint

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Background: Skin cancer and other pathophysiological conditions of the skin are caused by inflammation. Sunset Yellow (SY) exhibits immunomodulatory characteristics, evidenced by its capacity to partially inhibit the secretion of proinflammatory cytokines, regulate immune cell populations, and modulate the activation of lymphocytes. The aim of our study was to investigate the potential anti-inflammatory properties of SY through in-silico, in vitro & physiochemical test systems, and to investigate further its implications in DMBA/TPA-induced two-step skin carcinogenesis model. Result: In vitro experiments showed that pre-treatment of SY significantly enhanced the cell viability of HaCaT cells when exposed to tertiary-Butyl Hydrogen Peroxide (tBHP). The increase in cell viability was accompanied by decreased levels of ROS, restoration of deregulated mitochondrial membrane potential, and significantly reduced DNA damage in (SY+ tBHP) treated cells. Further mechanistic investigation revealed that SY exhibited antioxidant properties. Through potentiometric titrations, the standard reduction potential (Eº) of SY was observed to be 0.211V. Additionally, this result was also confirmed using DPPH chemical antioxidant activity test. Next, we tested the implications of these findings in DMBA/TPA-induced two-step skin carcinogenesis animal model. The mice received topical application of SY (0.025%, 0.05%, and 0.1%) in conjunction with DMBA/TPA treatment for 21 weeks. The tumor incidence and body weight were evaluated at regular time intervals. SY remarkably reduced the tumor average latency period, tumor incidence, tumor yield and tumor burden in a dose-dependent manner. Finally, several in silico targets of SY were also identified, which could shed some light on the molecular mechanism triggered by SY as an antioxidant and chemo-preventive agent. Conclusions: Conclusively, we repurposed SY for the antioxidant and anti-genotoxic properties along with its chemoprotective effect on skin cancer.

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Assessment of coarse, fine, and ultrafine particulate matter at different microenvironments of fire stations

et al. 2023

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Physicochemical characterization and oxidative potential of size fractionated Particulate Matter: Uptake, genotoxicity and mutagenicity in V-79 cells

Cited by 6 publications

References 55 publications

Sunset Yellow protects against oxidative damage and exhibits chemoprevention in chemically induced skin cancer model

Sunset Yellow protects against oxidative damage and exhibits chemoprevention in chemically induced skin cancer model

Sunset Yellow protects against oxidative damage in human keratinocyte-derived HaCaT cells and exhibits chemopreventive efficacy in DMBA/TPA-induced skin carcinogenesis

Assessment of coarse, fine, and ultrafine particulate matter at different microenvironments of fire stations

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